Basic Vector Information
- Vector Name:
- pMG401
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4469 bp
- Type:
- Cloning vector
- Replication origin:
- p15A ori
- Source/Author:
- Emge P.
pMG401 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMG401 vector Sequence
LOCUS 40924_30770 4469 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pMG401, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4469)
AUTHORS Emge P.
TITLE Quorum sensing resilience against fluid flow
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4469)
AUTHORS Emge P.
TITLE Direct Submission
JOURNAL Submitted (30-APR-2015) Health Science and Technology, ETH Zurich,
Vladimir-Prelog-Weg 1-5/10, Zurich 8093, Switzerland
REFERENCE 3 (bases 1 to 4469)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4469)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(30-APR-2015) Health Science and Technology, ETH Zurich,
Vladimir-Prelog-Weg 1-5/10, Zurich 8093, Switzerland"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4469
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(70..786)
/gene="lasR"
/label=lasR
/note="Transcriptional activator protein LasR from
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP
104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
Accession#: P25084"
RBS 793..804
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
RBS 985..996
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 1003..1605
/gene="lasI"
/label=lasI
/note="Acyl-homoserine-lactone synthase from Pseudomonas
aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 /
JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1). Accession#:
P33883"
RBS 1713..1724
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 1731..2444
/label=yeGFP
/note="yeast-enhanced green fluorescent protein"
terminator 2535..2621
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin complement(2791..3336)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
terminator complement(3450..3544)
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(3578..4369)
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
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