LOCUS       V004746                 4469 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004746
VERSION     V004746
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Emge P.
  TITLE     Quorum sensing resilience against fluid flow
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4469)
  AUTHORS   Emge P.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-APR-2015) Health Science and Technology, ETH Zurich,
            Vladimir-Prelog-Weg 1-5/10, Zurich 8093, Switzerland
REFERENCE   3  (bases 1 to 4469)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4469)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-APR-2015) Health Science and Technology, ETH Zurich,
            Vladimir-Prelog-Weg 1-5/10, Zurich 8093, Switzerland"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(70..786)
                     /gene="lasR"
                     /label="Transcriptional activator protein LasR"
                     /note="Transcriptional activator protein LasR from
                     Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP
                     104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
                     Accession#: P25084"
     RBS             793..804
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     RBS             985..996
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             1003..1605
                     /gene="lasI"
                     /label="Acyl-homoserine-lactone synthase"
                     /note="Acyl-homoserine-lactone synthase from Pseudomonas
                     aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 /
                     JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1). Accession#:
                     P33883"
     RBS             1713..1724
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             1731..2444
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     terminator      2535..2621
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     rep_origin      complement(2791..3336)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     terminator      complement(3450..3544)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(3578..4369)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
ORIGIN
        1 ccaaccttac cagagggcgc cccagctggc aattccgacg tcattgcgtg cacctgagag
       61 gcaagatcag agagtaataa gacccaaatt aacggccata atggccgcta cgcggcggga
      121 ggtcacaccg aacttccgcc gaatatttcc catatggaag ttcacattgg cttccgagca
      181 gttgcagata accgatatct cccaactggt cttgccgatg gcgcaccact gcaacacttc
      241 cttctcccgg ctggtcagaa ccaccggttt gctgaccgga tgttcgaagg ccagtccggc
      301 accgctctgc agtgcgtagt ccttgagcat ccacagggtc ggcaggaccg actccatgaa
      361 acggttggcc tcggcccggt tttccgcttc cacgctgagg ctcagcgcgc cgagttcgcc
      421 gcgagcacca tgcagcggca tggtcagccc atacaccagg ccggcggccg aggcttcctc
      481 gaagaactcg tgctgctttc gcgtctggta gatggacggt tcccagaaaa tcggcagtac
      541 gctctgggta cagtgactga ccgtcgggtc gacccgcgcg tagccagccc ggtcgtaatg
      601 ctcgcgccag gcggccgggt agttgccgac gatgaaggcg ttctcgtagt cctggctgtc
      661 cttaggcaac aggccgaaca ggatcttcga gaatccaagg tcgctcgcca tcttctgcag
      721 gatggcgctc cactccaatt ttccacttga gcgttccagc tcaagaaaac cgtcaaccaa
      781 ggccatgctt aatttctcct ctttaatgct ctcttccggg cgctatcatg ccataccgcg
      841 aaaggttttg caccattcga tggtgtcaac gtaatctaga gctagcacga caggtttccc
      901 gatcgcaact agcaaatgag atagatttcg gtgaacccgg acccttgcta ggctcgaaga
      961 aacgtactcg actctagagg atccaaagag gagaaattaa gcatgatcgt acaaattggt
     1021 cggcgcgaag agttcgataa aaaactgctg ggcgagatgc acaagttgcg tgctcaagtg
     1081 ttcaaggagc gcaaaggctg ggacgttagt gtcatcgacg agatggaaat cgatggttat
     1141 gacgcactca gtccttatta catgttgatc caggaagata ctcctgaagc ccaggttttc
     1201 ggttgctggc gaattctcga taccactggc ccctacatgc tgaagaacac cttcccggag
     1261 cttctgcacg gcaaggaagc gccttgctcg ccgcacatct gggaactcag ccgtttcgcc
     1321 atcaactctg gacagaaagg ctcgctgggc ttttccgact gtacgctgga ggcgatgcgc
     1381 gcgctggccc gctacagcct gcagaacgac atccagacgc tggtgacggt aaccaccgta
     1441 ggcgtggaga agatgatgat ccgtgccggc ctggacgtat cgcgcttcgg tccgcacctg
     1501 aagatcggca tcgagcgcgc ggtggccttg cgcatcgaac tcaatgccaa gacccagatc
     1561 gcgctttacg ggggagtgct ggtggaacag cgactggcgg tttcatgacg gggacctgtc
     1621 ggctcgcgcc ggcgcgttct ctgtcggaga gggagcgcct ggcgtcgatg ccgaatccgc
     1681 tgtaaggcaa cgaaaactag gtacctagaa ttaaagagga gaaattaagc atgcgtaaag
     1741 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
     1801 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
     1861 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
     1921 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt
     1981 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
     2041 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
     2101 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
     2161 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta
     2221 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
     2281 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
     2341 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
     2401 taacagctgc tgggattaca catggcatgg atgaactata caaaaggcct gcagcaaacg
     2461 acgaaaacta cgctttagta gcttaataag cttaattagc tgacctacta gtcggccgtc
     2521 tcgagtagag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     2581 tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccgccc tagacctagg
     2641 ggatatattc cgcttcctcg ctcactgact cgctacgctc ggtcgttcga ctgcggcgag
     2701 cggaaatggc ttacgaacgg ggcggagatt tcctggaaga tgccaggaag atacttaaca
     2761 gggaagtgag agggccgcgg caaagccgtt tttccatagg ctccgccccc ctgacaagca
     2821 tcacgaaatc tgacgctcaa atcagtggtg gcgaaacccg acaggactat aaagatacca
     2881 ggcgtttccc cctggcggct ccctcgtgcg ctctcctgtt cctgcctttc ggtttaccgg
     2941 tgtcattccg ctgttatggc cgcgtttgtc tcattccacg cctgacactc agttccgggt
     3001 aggcagttcg ctccaagctg gactgtatgc acgaaccccc cgttcagtcc gaccgctgcg
     3061 ccttatccgg taactatcgt cttgagtcca acccggaaag acatgcaaaa gcaccactgg
     3121 cagcagccac tggtaattga tttagaggag ttagtcttga agtcatgcgc cggttaaggc
     3181 taaactgaaa ggacaagttt tggtgactgc gctcctccaa gccagttacc tcggttcaaa
     3241 gagttggtag ctcagagaac cttcgaaaaa ccgccctgca aggcggtttt ttcgttttca
     3301 gagcaagaga ttacgcgcag accaaaacga tctcaagaag atcatcttat taatcagata
     3361 aaatattact agatttcagt gcaatttatc tcttcaaatg tagcacctga agtcagcccc
     3421 atacgatata agttgttact agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa
     3481 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg
     3541 agtccaagcg agctctcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat
     3601 agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag
     3661 cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc
     3721 ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca
     3781 tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc
     3841 gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat
     3901 catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg
     3961 cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag
     4021 ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca
     4081 cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc
     4141 aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca
     4201 gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga
     4261 acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct
     4321 ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc
     4381 ctcatcctgt ctcttgatca gatcttgatc ccctgcgcca tcagatcctt ggcggcaaga
     4441 aagccatcca gtttactttg cagggcttc
//
