Basic Vector Information
- Vector Name:
- pMF280
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9651 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Freitag M, Hickey PC, Raju NB, Selker EU, Read ND.
pMF280 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMF280 vector Sequence
LOCUS 40924_30725 9651 bp DNA circular SYN 18-DEC-2018 DEFINITION Expression vector pMF280, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9651) AUTHORS Freitag M, Hickey PC, Raju NB, Selker EU, Read ND. TITLE GFP as a tool to analyze the organization, dynamics and function of nuclei and microtubules in Neurospora crassa JOURNAL Unpublished REFERENCE 2 (bases 1 to 9651) AUTHORS Freitag M, Selker EU. TITLE Direct Submission JOURNAL Submitted (13-APR-2004) Inst. of Mol. Biology, University of Oregon, OR, USA REFERENCE 3 (bases 1 to 9651) TITLE Direct Submission REFERENCE 4 (bases 1 to 9651) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (13-APR-2004) Inst. of Mol. Biology, University of Oregon, OR, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..9651 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 363..467 /label=AmpR promoter CDS 468..1325 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 1499..2087 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 2345..2363 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 2638..2649 /codon_start=1 /label=Factor Xa site /note="Factor Xa recognition and cleavage site" /translation="IEGR" primer_bind 4981..4997 /label=SK primer /note="common sequencing primer, one of multiple similar variants" CDS 6185..6901 /codon_start=1 /label=EGFP /note="enhanced GFP" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(6925..6941) /label=KS primer /note="common sequencing primer, one of multiple similar variants" promoter complement(6971..6989) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" promoter complement(join(9650..9651,1..17)) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase"
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