LOCUS       40924_30725        9651 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMF280, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9651)
  AUTHORS   Freitag M, Hickey PC, Raju NB, Selker EU, Read ND.
  TITLE     GFP as a tool to analyze the organization, dynamics and function of 
            nuclei and microtubules in Neurospora crassa
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9651)
  AUTHORS   Freitag M, Selker EU.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2004) Inst. of Mol. Biology, University of Oregon,
            OR, USA
REFERENCE   3  (bases 1 to 9651)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9651)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-APR-2004) Inst. of Mol. Biology, University of Oregon, OR, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9651
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        363..467
                     /label=AmpR promoter
     CDS             468..1325
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2638..2649
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     primer_bind     4981..4997
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6185..6901
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6925..6941)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6971..6989)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(join(9650..9651,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat
      121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1621 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1861 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg
     2341 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact
     2401 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt
     2461 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta
     2521 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat
     2581 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc
     2641 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac
     2701 tccacacacc cacgctgaat ggcacaacca ctgtaggcct agcggtccca gctccccgta
     2761 accacgcagc cccagcgacc gaggtcatca tgacactatg acgacaatct ggcgggagac
     2821 ttgggaaaat cgatgacggg cacctacctt gggaacaata gagcaagtcc agtcaacgcc
     2881 ctagggaagg gggaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta
     2941 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga
     3001 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg
     3061 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc
     3121 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc
     3181 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg
     3241 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa
     3301 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac
     3361 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat
     3421 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc
     3481 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac
     3541 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa
     3601 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac
     3661 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg
     3721 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg
     3781 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc
     3841 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta
     3901 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct
     3961 gaaacccgtt atagatatgt atagatttga cggggtagct tggcccttaa tacgactcac
     4021 tatagggcga attggagctc caccgcggtg gcggccgcta gaaggagcag tccatctgcg
     4081 tgaatcacga gagaatcagc tactttgaat cgatggatgc agctaccaga agtcactcag
     4141 ttcgttcaaa gccacatcac tgggcacttc cattgggaca ggcattgatc ggacgagacc
     4201 gacttctggc cgctttcaac agccacatta tatccatgtc acggctacgc gtcggccttc
     4261 ggtaacagaa aagcacacag acagcgattg tgacatggat tcgggcaaag cattggtggt
     4321 cgcaccaggt catcctgagt gtgcagtggc tgctattcag atttcatcta actgcgggag
     4381 aggggttcaa aggggcgtga cgtcacagac aacgggtgaa ggacgaagat tgcctcactt
     4441 ctttgctagc aatttgcctg caaagaaggc gcacatgaca agcaaacaac tgggaaacca
     4501 ctattgaata cccacaatgc aaagctcgga aggtacgtct tgattgcagt gtgtcgagtg
     4561 tcaaaaaaga agcaagtgtt catgcaagcc aaaattggca cctcctccac ttctccgagt
     4621 gccccacccg aacctccagg cgagatggcc ggaacatacc atccgcgttg ggattatgac
     4681 gtatctcctt cttcttcaca tgattccatc ccgttgttgc ttgtttgcga gctgtgacgg
     4741 gagatcgtag atgccacttc gggccaggca ggcagtgcag gcagccagga acacaagctt
     4801 ccaacttggt catctcgatt gccgattaag ggaaccaaat gcctatataa gactgtcctc
     4861 ccacctcccc aataccattc ttttcttctt ccatcatcag ccaacaaagc aatcacatct
     4921 tcactacttc aaatcaacac aacactcaaa ccactttcac aacccctcac atcaaccaaa
     4981 tctagaacta gtggatccac catgcctccc aagaagaccg agaccaaggc cgccgatgcc
     5041 tctgccgccg ccgctcctgc tcccgccgct gctcccacct ctgctcccaa gaccaagagc
     5101 cccagcaccc atgcctctta ccttgtaagc tttacttaat ccccaagaga aacgcatctt
     5161 ttgctaacat tcaaacagga catgatcacg gacgctattg ttgcggtatt taaaccccct
     5221 cattatcttt gttgtggcgt gttttggttg tgggcgcttc tctggtcggt gagcaaaatg
     5281 agacaattgc tctcatgatg cacgcaacca agagtcaccc acaccactca ccaccctttt
     5341 actgtccctc agtcgtgctg attccttctt tttgtcatgc tacagctcaa ggaccgcgct
     5401 ggatctaggt atgtgccatt tttctgctta cttttctcat tttttcacca gggctttgcc
     5461 aagccacacc gcaaaccttg cgggcccttc ctacggtcgc accgatgacc actttcattc
     5521 ttatttccat gacgcgatga aggatcacaa catgttgcta acgcacacca cagccgtcaa
     5581 gctctcaaga agtatgtccg tgccaacaac acgctcggca acgtcaccga caacatgttc
     5641 gactcgctct tcaacaaggc cttgaagaac ggtgtcgaca agggtgtctt tgagcagccc
     5701 aagggtatgt caccacccac atgacgcatc atctccttgt attactcttt gcatgctcac
     5761 acacgatata ggtccttccg gtggcaccaa gcttgccaag aaggtcgcta agcctgctcc
     5821 caagaaggct gctcccaaga aggagaccaa ggagaagaag cccgctgccg ctaagaagga
     5881 gggtgccgcc aagaaggaga ccaaggagaa gaaggctcct gctgccaaga aggcggctgc
     5941 ccccaagaag gctgctgctc ccaagaagga ggtcaaggag aagaaggcgg ctgcccccaa
     6001 gaagaaggcc gctgctcctg ccgttgccga caaggagacc gtcctcacca agaccaagtc
     6061 tggccgtgtc gccaagagca ccgctaagcc tgctgccgcc aagaaggccg ctgctcccaa
     6121 gaaggctgcc gccagcaaga aggccgagaa ggccgagccc gctgccgaga aggcattaat
     6181 taacatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     6241 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     6301 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     6361 caccctcgtg accaccttca cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     6421 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     6481 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     6541 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     6601 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     6661 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     6721 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     6781 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     6841 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     6901 gtaagaattc gatatcaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccca
     6961 gcttttgttc cctttagtga gggttaatta gggcgtggac ggctaatggg gtctgaatgc
     7021 taaaagacac catttcccac actccctctg atttttttta tggttttttt ttatcttcat
     7081 ttttcgccgt cttttattcg gttgtttggg aggttcacat gcaacgtgca agactgcaag
     7141 ttttttgaga tgggcgcttc atgcaatgca gcatcactgc agggacattg tgacatccac
     7201 gtttcgatcg tctgtgcttc ccccgttcat gtacgcatga tctgaaaaaa aaaaaaaaaa
     7261 aaaaggttga tgtaataatg aaaataaaca cccagggacg agcacctcat ctgctggaat
     7321 agggagaaaa actcaagggc cgtttgggtc tccacactta ccaacagatt catgtactga
     7381 tcttcagcct gtacagaaca gtacaatgtc caaaactcgt tatcattact ctatgtgccc
     7441 aaaatgaccc agatatacat caacccacta catccttttg ttcttgtttt tttttttttt
     7501 ttttgctact tcgaaaagaa tttccgttta gttgctcttg ctcatgtgct caagacggat
     7561 gctgaccgcc cttctgtgag cctcgagctc ctcaacctta gccaactgca tgacagcctg
     7621 gccgacgttt ttgagaccct cggcagtcaa gttggagctg gtaatgtgct tgacgaacga
     7681 ggcgaaattg acgccagagt actgcttgcc aaagccatag gtagctatgt gtcttgacgt
     7741 tagcagaaga atctgtgaag cggggctatt tatgatatgt aacttacgca gcgagtggtt
     7801 aacaccagca gagtaatcgc caacggactc aggagtccag gcgccaatga agacactacc
     7861 ggcgttcatg acaagatcga cagccttctc ggcctccttg atctggagga tcaagtgctc
     7921 aggagcgtac ttgttgctga gctccatggc ctcctcgacg gtcttgacct gcacggtgat
     7981 cgagtgggcg atggagccac ggacaatctg gacgcgagga agctccgtag cctgacggtg
     8041 aacctcgtcc tcaatagcct gaagatgctc ctcgtcgagg tcaatagcga tcaggatgac
     8101 ctgactgtca acgccgtgct cagcctggga caggagatcc gaggcaacga acgcggggtt
     8161 ggcgtccttg tcagcgatga ccagcacctc ggacgggcca gcgggcatgt caataccaac
     8221 ggcagcgttg gtgtcgttgc tgacgaacat cttggcagca gtgacgaact ggttaccggg
     8281 gccgagaatc ttgtcgacct tggtgatgct ctcggtgccg taggccatgg cagctacggc
     8341 ctgggcaccg ccggcaagca cgatggactc ggccccaacc ttgtgagcga cgtggacaat
     8401 ctcgggagtg atggttccgt cggcgcgggg aggagaggcg aacacaatct tgttgcagcc
     8461 ggcgaccatg gcgggaacac ccagcataag ggcagtgctg gggagaacgg cggtaccgcc
     8521 ggggatgtag cagccgacgg cctcgatggg acgagagaaa cggctgcaga caacaccggg
     8581 catggtctcg acctggaggg gcttctcctc cttctgggcg gcgtggaact tgcggatgtt
     8641 ctcgaaggac acgtcgatgg cagcaatggt ctcctcaggg agctgcataa gctccttggg
     8701 gaagggcgcc ttcaggacgg ggctagtaag agaggtagcc ttctcgaact tgtgagtgta
     8761 cgacagaaca gccttgtcgc cgttcttgcg gacgtcctcg atgatgggga caatgatctt
     8821 gtagatggca tcggacgact tttgcgcagg acgcttgaga gcagcatcga gctcctcggt
     8881 agagaccttg gaggcgtcga aacgtctcat ggtgatcttc tcaggggtgg tctcctgggc
     8941 ggcctccttg gtgacagggg ccgaagtggc agcgagagcg gacgccgcag gcttgatgcc
     9001 ctccttctca gcccacttac ccttagcatc tccagtcctg cgcttgacct tccagctctt
     9061 ggcgtcaagg ctcctttcga tatcggcaag agtaacgccg gcagcaacgg ccctggtaag
     9121 agcaaagtag aagagatcgg cagcctcaaa ggcgatttcc tggggggtct gagcggtgca
     9181 gagctcctca gcctcctcca tgatcttggc ccggactagc ttctcatcgg agaagagacg
     9241 ggcagtgtag gagccctcgg gggcagactg tttcctcgaa atcaaagtct gctcgagctt
     9301 gggaaggcct ttgagctgac caaagcagcc ggactgatcg aggtggcaga aacgaccctt
     9361 ctgcttcacg acaaacttga gagcatcgtt atcgcagtca agcgagatgc ggacgagctc
     9421 ctgagtgtct ccggaagtag cacccttgta ccagagaccg cgcttccggc tctgatagac
     9481 accagtctgt gtcctgaggg cctcgttcac actctcggca ctgctgtaga ccagacccag
     9541 agcagtgtcg tgctcatcaa cgacaacggt ggggagaaga ccatcgggac ggtcagactt
     9601 ccagacgctc gagaggatgg tggagatggc aagcttcagc tgctcgagtt c
//
