pMF272 vector (V004757)

LOCUS       40924_30715        8479 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMF272, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Freitag M, Hickey PC, Raju NB, Selker EU, Read ND.
  TITLE     GFP as a tool to analyze the organization, dynamics and function of 
            nuclei and microtubules in Neurospora crassa
  JOURNAL   Fungal Genet. Biol. 41 (10), 897-910 (2004)
  PUBMED    15341912
REFERENCE   2  (bases 1 to 8479)
  AUTHORS   Freitag M, Selker EU.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2004) Inst. of Mol. Biology, University of Oregon,
            OR, USA
REFERENCE   3  (bases 1 to 8479)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8479)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet. Biol."; date: "2004"; volume: "41"; issue: "10"; pages: 
            "897-910"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-APR-2004) Inst. of Mol. Biology, University of Oregon, OR, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        363..467
                     /label=AmpR promoter
     CDS             468..1325
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2638..2649
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     primer_bind     4981..4997
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5013..5729
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5753..5769)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(5799..5817)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(join(8478..8479,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"