LOCUS       40924_30715        8479 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pMF272, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8479)
  AUTHORS   Freitag M, Hickey PC, Raju NB, Selker EU, Read ND.
  TITLE     GFP as a tool to analyze the organization, dynamics and function of 
            nuclei and microtubules in Neurospora crassa
  JOURNAL   Fungal Genet. Biol. 41 (10), 897-910 (2004)
  PUBMED    15341912
REFERENCE   2  (bases 1 to 8479)
  AUTHORS   Freitag M, Selker EU.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2004) Inst. of Mol. Biology, University of Oregon,
            OR, USA
REFERENCE   3  (bases 1 to 8479)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8479)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet. Biol."; date: "2004"; volume: "41"; issue: "10"; pages: 
            "897-910"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-APR-2004) Inst. of Mol. Biology, University of Oregon, OR, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8479
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        363..467
                     /label=AmpR promoter
     CDS             468..1325
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1499..2087
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2345..2363
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2638..2649
                     /codon_start=1
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
                     /translation="IEGR"
     primer_bind     4981..4997
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             5013..5729
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5753..5769)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(5799..5817)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(join(8478..8479,1..17))
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc
       61 gttctaacga caatatgtcc atatggtgca ctctcagtac aatctgctct gatgccgcat
      121 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
      181 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
      241 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
      301 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
      361 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
      421 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
      481 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
      541 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
      601 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
      661 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
      721 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
      781 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
      841 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
      901 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
      961 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1021 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1081 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     1141 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     1201 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     1261 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     1321 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     1381 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     1441 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     1501 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1561 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1621 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     1681 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1741 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1801 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1861 acaccgaact gagataccta cagcgtgagc attgagaaag cgccacgctt cccgaaggga
     1921 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1981 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2041 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     2101 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     2161 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     2221 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     2281 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg caggttaacc tggcttatcg
     2341 aaattaatac gactcactat agggagaccg gcagatccaa tgcggatgga ttcgctcact
     2401 cggtgccgcg gcggtgcgaa gttcctgccg tggattgggg gattgcacag acggtggagt
     2461 gcttgctaca gctaggtacc cagttgcaca ccaaacggtg cccctgtgcc cctgcgtcta
     2521 ggtgtgtcct gtgagcaact gcgcccgcgc gcggcaccgg gaagctgcgg gaacctcaat
     2581 ggatgattgg cgccagatcc ccggcatcac ctcagcacat gggcagcccg agaaggaatc
     2641 gagggtagaa gatattccag ggtttccgct agcacaactt ccaagcgcct ctaccgctac
     2701 tccacacacc cacgctgaat ggcacaacca ctgtaggcct agcggtccca gctccccgta
     2761 accacgcagc cccagcgacc gaggtcatca tgacactatg acgacaatct ggcgggagac
     2821 ttgggaaaat cgatgacggg cacctacctt gggaacaata gagcaagtcc agtcaacgcc
     2881 ctagggaagg gggaaaaaaa aaaagagccc attctcacaa ggagcaacac tgtgctgcta
     2941 tcatagtaca ctacaagcaa agtcgggatg ttgctagaat ccgtcggcta cgtcggtaga
     3001 tacctcccat aggcagtaag tctaggtatc caatttatag ttccgcgtat ttcttcgtcg
     3061 aacggggcac agcaaagcct aacagccgca gtgcctcgca accaacacca agcagtaatc
     3121 tcatccatcc atacagatcc tcccacaata tctttacatg cgcacttgcg ctgtgttagc
     3181 agtttaggtt ccactaccaa ggctgacacg agcagcactg acagcaaact gctgcttggg
     3241 gccaagaaat tggcagtttg ctgagcacct acatatcagt acacatcaac cctggtttaa
     3301 ttgttagcct ggtactgtta accgtcatga acgaggacgt ggaggaagaa ccacgctaac
     3361 aattgaacca gggttgatgt atacctctct taccatattt gcttgcttcc acgtccctat
     3421 cctcaagcga ttttgttctt gactttttgt atttcaacgc aaaggccatt caggtttctc
     3481 atgttcaacc ctttggatgg cgatgccgtt cgtcccagga cctcgtgacc tttgaaagac
     3541 gagaaatata tcccaaagca acttctgctt atggacaagc aactgcatgg cggcccacaa
     3601 ctcatatctc gcgtaatcat ccaaacgcta ggcccgagta ctctactgtt ctcgagacac
     3661 ctaccataat atgtacccaa tcagacatga caacagtcaa ccaaacatac cctgatccgg
     3721 ccaaaccctg acatccctag atgatggtta ggcacacaaa tggaaaacgg gactcgttgg
     3781 ttaggtggga acgcttgtat tgcagccagc caaccgactc tcctcgtccc tctcgagtcc
     3841 cgttattgcc gtttgaccca gccgcccagc cgccaaacag ctgagggagc caatggtcta
     3901 atacaccgtc caacggcccc agtgatgttt caaacttgat tgacagcgaa cgaaacccct
     3961 gaaacccgtt atagatatgt atagatttga cggggtagct tggcccttaa tacgactcac
     4021 tatagggcga attggagctc caccgcggtg gcggccgcta gaaggagcag tccatctgcg
     4081 tgaatcacga gagaatcagc tactttgaat cgatggatgc agctaccaga agtcactcag
     4141 ttcgttcaaa gccacatcac tgggcacttc cattgggaca ggcattgatc ggacgagacc
     4201 gacttctggc cgctttcaac agccacatta tatccatgtc acggctacgc gtcggccttc
     4261 ggtaacagaa aagcacacag acagcgattg tgacatggat tcgggcaaag cattggtggt
     4321 cgcaccaggt catcctgagt gtgcagtggc tgctattcag atttcatcta actgcgggag
     4381 aggggttcaa aggggcgtga cgtcacagac aacgggtgaa ggacgaagat tgcctcactt
     4441 ctttgctagc aatttgcctg caaagaaggc gcacatgaca agcaaacaac tgggaaacca
     4501 ctattgaata cccacaatgc aaagctcgga aggtacgtct tgattgcagt gtgtcgagtg
     4561 tcaaaaaaga agcaagtgtt catgcaagcc aaaattggca cctcctccac ttctccgagt
     4621 gccccacccg aacctccagg cgagatggcc ggaacatacc atccgcgttg ggattatgac
     4681 gtatctcctt cttcttcaca tgattccatc ccgttgttgc ttgtttgcga gctgtgacgg
     4741 gagatcgtag atgccacttc gggccaggca ggcagtgcag gcagccagga acacaagctt
     4801 ccaacttggt catctcgatt gccgattaag ggaaccaaat gcctatataa gactgtcctc
     4861 ccacctcccc aataccattc ttttcttctt ccatcatcag ccaacaaagc aatcacatct
     4921 tcactacttc aaatcaacac aacactcaaa ccactttcac aacccctcac atcaaccaaa
     4981 tctagaacta gtggatcccc gggttaatta acatggtgag caagggcgag gagctgttca
     5041 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     5101 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     5161 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcacc tacggcgtgc
     5221 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     5281 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     5341 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     5401 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     5461 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     5521 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     5581 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     5641 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     5701 tcactctcgg catggacgag ctgtacaagt aagaattcga tatcaagctt atcgataccg
     5761 tcgacctcga gggggggccc ggtacccagc ttttgttccc tttagtgagg gttaattagg
     5821 gcgtggacgg ctaatggggt ctgaatgcta aaagacacca tttcccacac tccctctgat
     5881 tttttttatg gttttttttt atcttcattt ttcgccgtct tttattcggt tgtttgggag
     5941 gttcacatgc aacgtgcaag actgcaagtt ttttgagatg ggcgcttcat gcaatgcagc
     6001 atcactgcag ggacattgtg acatccacgt ttcgatcgtc tgtgcttccc ccgttcatgt
     6061 acgcatgatc tgaaaaaaaa aaaaaaaaaa aaggttgatg taataatgaa aataaacacc
     6121 cagggacgag cacctcatct gctggaatag ggagaaaaac tcaagggccg tttgggtctc
     6181 cacacttacc aacagattca tgtactgatc ttcagcctgt acagaacagt acaatgtcca
     6241 aaactcgtta tcattactct atgtgcccaa aatgacccag atatacatca acccactaca
     6301 tccttttgtt cttgtttttt tttttttttt ttgctacttc gaaaagaatt tccgtttagt
     6361 tgctcttgct catgtgctca agacggatgc tgaccgccct tctgtgagcc tcgagctcct
     6421 caaccttagc caactgcatg acagcctggc cgacgttttt gagaccctcg gcagtcaagt
     6481 tggagctggt aatgtgcttg acgaacgagg cgaaattgac gccagagtac tgcttgccaa
     6541 agccataggt agctatgtgt cttgacgtta gcagaagaat ctgtgaagcg gggctattta
     6601 tgatatgtaa cttacgcagc gagtggttaa caccagcaga gtaatcgcca acggactcag
     6661 gagtccaggc gccaatgaag acactaccgg cgttcatgac aagatcgaca gccttctcgg
     6721 cctccttgat ctggaggatc aagtgctcag gagcgtactt gttgctgagc tccatggcct
     6781 cctcgacggt cttgacctgc acggtgatcg agtgggcgat ggagccacgg acaatctgga
     6841 cgcgaggaag ctccgtagcc tgacggtgaa cctcgtcctc aatagcctga agatgctcct
     6901 cgtcgaggtc aatagcgatc aggatgacct gactgtcaac gccgtgctca gcctgggaca
     6961 ggagatccga ggcaacgaac gcggggttgg cgtccttgtc agcgatgacc agcacctcgg
     7021 acgggccagc gggcatgtca ataccaacgg cagcgttggt gtcgttgctg acgaacatct
     7081 tggcagcagt gacgaactgg ttaccggggc cgagaatctt gtcgaccttg gtgatgctct
     7141 cggtgccgta ggccatggca gctacggcct gggcaccgcc ggcaagcacg atggactcgg
     7201 ccccaacctt gtgagcgacg tggacaatct cgggagtgat ggttccgtcg gcgcggggag
     7261 gagaggcgaa cacaatcttg ttgcagccgg cgaccatggc gggaacaccc agcataaggg
     7321 cagtgctggg gagaacggcg gtaccgccgg ggatgtagca gccgacggcc tcgatgggac
     7381 gagagaaacg gctgcagaca acaccgggca tggtctcgac ctggaggggc ttctcctcct
     7441 tctgggcggc gtggaacttg cggatgttct cgaaggacac gtcgatggca gcaatggtct
     7501 cctcagggag ctgcataagc tccttgggga agggcgcctt caggacgggg ctagtaagag
     7561 aggtagcctt ctcgaacttg tgagtgtacg acagaacagc cttgtcgccg ttcttgcgga
     7621 cgtcctcgat gatggggaca atgatcttgt agatggcatc ggacgacttt tgcgcaggac
     7681 gcttgagagc agcatcgagc tcctcggtag agaccttgga ggcgtcgaaa cgtctcatgg
     7741 tgatcttctc aggggtggtc tcctgggcgg cctccttggt gacaggggcc gaagtggcag
     7801 cgagagcgga cgccgcaggc ttgatgccct ccttctcagc ccacttaccc ttagcatctc
     7861 cagtcctgcg cttgaccttc cagctcttgg cgtcaaggct cctttcgata tcggcaagag
     7921 taacgccggc agcaacggcc ctggtaagag caaagtagaa gagatcggca gcctcaaagg
     7981 cgatttcctg gggggtctga gcggtgcaga gctcctcagc ctcctccatg atcttggccc
     8041 ggactagctt ctcatcggag aagagacggg cagtgtagga gccctcgggg gcagactgtt
     8101 tcctcgaaat caaagtctgc tcgagcttgg gaaggccttt gagctgacca aagcagccgg
     8161 actgatcgag gtggcagaaa cgacccttct gcttcacgac aaacttgaga gcatcgttat
     8221 cgcagtcaag cgagatgcgg acgagctcct gagtgtctcc ggaagtagca cccttgtacc
     8281 agagaccgcg cttccggctc tgatagacac cagtctgtgt cctgagggcc tcgttcacac
     8341 tctcggcact gctgtagacc agacccagag cagtgtcgtg ctcatcaacg acaacggtgg
     8401 ggagaagacc atcgggacgg tcagacttcc agacgctcga gaggatggtg gagatggcaa
     8461 gcttcagctg ctcgagttc
//
