Basic Vector Information
- Vector Name:
- pmET32b
- Antibiotic Resistance:
- Gentamicin
- Length:
- 4993 bp
- Type:
- Expression vector
- Replication origin:
- ori
- Source/Author:
- Ebrahimi Nik H, Bassami MR, Rad M, Mohri M, Hosseini Shahidi R.
- Promoter:
- Pc
pmET32b vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pmET32b vector Sequence
LOCUS 40924_30665 4993 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pmET32b, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4993)
AUTHORS Ebrahimi Nik H, Bassami MR, Rad M, Mohri M, Hosseini Shahidi R.
TITLE Nucleotide sequence of bacterial ghost creator vector 2
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4993)
AUTHORS Ebrahimi Nik H, Bassami MR, Rad M, Mohri M, Hosseini Shahidi R.
TITLE Direct Submission
JOURNAL Submitted (20-AUG-2013) Pathobiology, Biotechnology, Ferdowsi
Pardis, Azadi Sq., Mashhad, Khorasan Razavi 9177948974, Iran
REFERENCE 3 (bases 1 to 4993)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4993)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(20-AUG-2013) Pathobiology, Biotechnology, Ferdowsi Pardis, Azadi
Sq., Mashhad, Khorasan Razavi 9177948974, Iran"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4993
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 254..275
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS 1050..1238
/label=rop
/note="Rop protein, which maintains plasmids at low copy
number"
misc_feature 1343..1485
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(1671..2259)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 2347..2375
/label=Pc promoter
/note="class 1 integron promoter"
CDS 2564..3094
/label=GmR
/note="gentamycin acetyltransferase"
rep_origin complement(3256..3711)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
terminator complement(3748..3795)
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
CDS complement(3862..3879)
/label=6xHis
/note="6xHis affinity tag"
CDS complement(3892..4602)
/label=lambda repressor (ts)
/note="temperature-sensitive variant of the phage lambda
repressor"
regulatory 4650..4677
/label=lambda PR
/note="lambda PR"
/regulatory_class="promoter"
RBS 4691..4699
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
CDS 4706..4978
/gene="E"
/label=E
/note="Lysis protein E from Enterobacteria phage phiX174.
Accession#: P03639"
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