LOCUS       40924_30665        4993 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pmET32b, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Ebrahimi Nik H, Bassami MR, Rad M, Mohri M, Hosseini Shahidi R.
  TITLE     Nucleotide sequence of bacterial ghost creator vector 2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4993)
  AUTHORS   Ebrahimi Nik H, Bassami MR, Rad M, Mohri M, Hosseini Shahidi R.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-AUG-2013) Pathobiology, Biotechnology, Ferdowsi 
            Pardis, Azadi Sq., Mashhad, Khorasan Razavi 9177948974, Iran
REFERENCE   3  (bases 1 to 4993)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4993)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-AUG-2013) Pathobiology, Biotechnology, Ferdowsi Pardis, Azadi 
            Sq., Mashhad, Khorasan Razavi 9177948974, Iran"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4993
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    254..275
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             1050..1238
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    1343..1485
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1671..2259)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2347..2375
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             2564..3094
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     rep_origin      complement(3256..3711)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     terminator      complement(3748..3795)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(3862..3879)
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             complement(3892..4602)
                     /label=lambda repressor (ts)
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
     regulatory      4650..4677
                     /label=lambda PR
                     /note="lambda PR"
                     /regulatory_class="promoter"
     RBS             4691..4699
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4706..4978
                     /gene="E"
                     /label=E
                     /note="Lysis protein E from Enterobacteria phage phiX174. 
                     Accession#: P03639"
ORIGIN
        1 ggccgcaagc ttgtcaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt
       61 ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt
      121 ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc
      181 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      241 agcgcaacgc aattaatgta agttagctca ctcattaggc accgggatct cgaccgatgc
      301 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
      361 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
      421 gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
      481 cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
      541 gtttcggcga gaagcaggcc attatcgccg gcatggcggc cccacgggtg cgcatgatcg
      601 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg
      661 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag
      721 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag
      781 cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa
      841 cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc
      901 gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat
      961 catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga
     1021 acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca
     1081 tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg
     1141 cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca
     1201 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     1261 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     1321 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     1381 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg
     1441 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     1501 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1561 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1621 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1681 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1741 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1801 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1861 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1921 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1981 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2041 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2101 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2161 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2221 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2281 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgactaga
     2341 ggcgaattga cataagcctg ttcggttcgt aaactgtaat gcaagtagcg tatgcgctca
     2401 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     2461 atggcttgtt atgactgttt ttttgtacag tctatgcctc gggcatccaa gcagcaagcg
     2521 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagcaa
     2581 cgatgttacg cagcagggca gtcgccctaa aacaaagtta ggtggctcaa gtatgggcat
     2641 cattcgcaca tgtaggctcg gccctgacca agtcaaatcc atgcgggctg ctcttgatct
     2701 tttcggtcgt gagttcggag acgtagccac ctactcccaa catcagccgg actccgatta
     2761 cctcgggaac ttgctccgta gtaagacatt catcgcgctt gctgccttcg accaagaagc
     2821 ggttgttggc gctctcgcgg cttacgttct gcccaggttt gagcagccgc gtagtgagat
     2881 ctatatctat gatctcgcag tctccggcga gcaccggagg cagggcattg ccaccgcgct
     2941 catcaatctc ctcaagcatg aggccaacgc gcttggtgct tatgtgatct acgtgcaagc
     3001 agattacggt gacgatcccg cagtggctct ctatacaaag ttgggcatac gggaagaagt
     3061 gatgcacttt gatatcgacc caagtaccgc cacctaacaa ttcgttcaag ccgagatcgg
     3121 cttcccggcc gcaaactatt aactggcgaa ctacttactc tagcttccat ctcatgagcg
     3181 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     3241 gaaaagtgcc acctgaaatt gtaaacgtta atattttgtt aaaattcgcg ttaaattttt
     3301 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     3361 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     3421 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     3481 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     3541 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     3601 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     3661 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
     3721 caatccggat atagttcctc ctttcagcaa aaaacccctc aagacccgtt tagaggcccc
     3781 aaggggttat gctagttatt gctcagcggt ggcagcagcc aactcagctt cctttcgggc
     3841 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagcggtc agccaaacgt
     3901 ctcttcaggc cactgactag cgataacttt ccccacaacg gaacaactct cattgcatgg
     3961 gatcattggg tactgtgggt ttagtggttg taaaaacacc tgaccgctat ccctgatcag
     4021 tttcttgaag gtaaactcat cacccccaag tctggctatg cagaaatcac ctggctcaac
     4081 agcctgctca gggtcaacga gaattaacat tccgtcagga aagcttggct tggagcctgt
     4141 tggtgcggtc atggaattac cttcaacctc aagccagaat gcagaatcac tggctttttt
     4201 ggttgtgctt acccatctct ccgcatcacc tttggtaaag gttctaagct taggtgagaa
     4261 catccctgcc tgaacatgag aaaaaacagg gtactcatac tcacttctaa gtgacggctg
     4321 catactaacc gcttcataca tctcgtagat ttctctggcg attgaagggc taaattcttc
     4381 aacgctaact ttgagaattt ttgtaagcaa tgcggcgtta taagcattta atgcattgat
     4441 gccattaaat aaagcaccaa cgcctgactg ccccatcccc atcttgtctg cgacagattc
     4501 ctgggataag ccaagttcat ttttcttttt ttcataaatt gctttaaggc gacgtgcgtc
     4561 ctcaagctgc tcttgtgtta atggtttctt ttttgtgctc atacgttaaa tctatcaccg
     4621 caagggataa atatctaaca ccgtgcgtgt tgactatttt acctctggcg gtgataatgg
     4681 ttgcatgtac taaggaggta aacatatggt acgctggact ttgtgggata ccctcgcttt
     4741 cctgctcctg ttgagtttat tgctgccgtc attgcttatt atgttcatcc cgtcaacatt
     4801 caaacggcct gtctcatcat ggaaggcgct gaatttacgg aaaacattat taatggcgtc
     4861 gagcgtccgg ttaaagccgc tgaattgttc gcgcttacct tgcgtgtacg cgcaggaaac
     4921 actgacgttc ttgctgacgc agaagaaaac gtgcgtcaaa aattacgtgc agaaggagtg
     4981 aaaggaaaaa agc
//
