pmET32a vector (V004762)

LOCUS       V004762                 5159 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004762
VERSION     V004762
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Lagzian M, Bassami MR, Dehghani H.
  TITLE     In vitro responses of chicken macrophage-like monocytes following
            exposure to pathogenic and non-pathogenic E. coli ghosts loaded with
            a rational design of conserved genetic materials of influenza and
            Newcastle disease viruses
  JOURNAL   Vet. Immunol. Immunopathol. 176, 5-17 (2016)
   PUBMED   27288852
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Lagzian M, Bassami MR, Dehghani H.
  TITLE     Nucleotide sequence of bacterial ghost creator vector
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5159)
  AUTHORS   Lagzian M, Bassami MR, Dehghani H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2012) Biotechnology, Ferdowsi University of
            Mashhad, Azadi sq., Mashhad, Khorasan-Razavi 91779-48974, Iran
REFERENCE   4  (bases 1 to 5159)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5159)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Vet.
            Immunol. Immunopathol. 176, 5-17 (2016)"
            SGRef: number: 2; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 3; type: "Journal Article"; journalName: "Submitted
            (23-AUG-2012) Biotechnology, Ferdowsi University of Mashhad, Azadi
            sq., Mashhad, Khorasan-Razavi 91779-48974, Iran"
            SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    254..275
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             1050..1238
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    1343..1485
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1671..2259)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2433..3290)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3291..3395)
                     /label="AmpR promoter"
     rep_origin      complement(3422..3877)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     terminator      complement(3914..3961)
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     CDS             complement(4028..4045)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(4058..4768)
                     /label="lambda repressor (ts)"
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
     regulatory      4816..4843
                     /label="lambda PR"
                     /note="lambda PR"
                     /regulatory_class="promoter"
     RBS             4857..4865
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4872..5144
                     /gene="E"
                     /label="Lysis protein E"
                     /note="Lysis protein E from Enterobacteria phage phiX174.
                     Accession#: P03639"