LOCUS       40924_30660        5159 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pmET32a, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Lagzian M, Bassami MR, Dehghani H.
  TITLE     In vitro responses of chicken macrophage-like monocytes following 
            exposure to pathogenic and non-pathogenic E. coli ghosts loaded with
            a rational design of conserved genetic materials of influenza and 
            Newcastle disease viruses
  JOURNAL   Vet. Immunol. Immunopathol. 176, 5-17 (2016)
  PUBMED    27288852
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Lagzian M, Bassami MR, Dehghani H.
  TITLE     Nucleotide sequence of bacterial ghost creator vector
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 5159)
  AUTHORS   Lagzian M, Bassami MR, Dehghani H.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-AUG-2012) Biotechnology, Ferdowsi University of 
            Mashhad, Azadi sq., Mashhad, Khorasan-Razavi 91779-48974, Iran
REFERENCE   4  (bases 1 to 5159)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5159)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Vet. 
            Immunol. Immunopathol. 176, 5-17 (2016)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (23-AUG-2012) Biotechnology, Ferdowsi University of Mashhad, Azadi 
            sq., Mashhad, Khorasan-Razavi 91779-48974, Iran"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5159
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    254..275
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             1050..1238
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    1343..1485
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1671..2259)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2433..3290)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3291..3395)
                     /label=AmpR promoter
     rep_origin      complement(3422..3877)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     terminator      complement(3914..3961)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(4028..4045)
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             complement(4058..4768)
                     /label=lambda repressor (ts)
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
     regulatory      4816..4843
                     /label=lambda PR
                     /note="lambda PR"
                     /regulatory_class="promoter"
     RBS             4857..4865
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             4872..5144
                     /gene="E"
                     /label=E
                     /note="Lysis protein E from Enterobacteria phage phiX174. 
                     Accession#: P03639"
ORIGIN
        1 ggccgcaagc ttgtcaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt
       61 ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt
      121 ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc
      181 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      241 agcgcaacgc aattaatgta agttagctca ctcattaggc accgggatct cgaccgatgc
      301 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
      361 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
      421 gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
      481 cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
      541 gtttcggcga gaagcaggcc attatcgccg gcatggcggc cccacgggtg cgcatgatcg
      601 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg
      661 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag
      721 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag
      781 cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa
      841 cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc
      901 gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat
      961 catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga
     1021 acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca
     1081 tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg
     1141 cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca
     1201 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     1261 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     1321 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     1381 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg
     1441 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     1501 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1561 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1621 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1681 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1741 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1801 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1861 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1921 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1981 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2041 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2101 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2161 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2221 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2281 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     2341 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     2401 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     2461 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     2521 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     2581 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     2641 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     2701 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg
     2761 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     2821 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     2881 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     2941 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     3001 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     3061 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     3121 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     3181 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     3241 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     3301 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     3361 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     3421 gaaattgtaa acgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca
     3481 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag
     3541 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc
     3601 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc
     3661 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc
     3721 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa
     3781 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc
     3841 acacccgccg cgcttaatgc gccgctacag ggcgcgtccc attcgccaat ccggatatag
     3901 ttcctccttt cagcaaaaaa cccctcaaga cccgtttaga ggccccaagg ggttatgcta
     3961 gttattgctc agcggtggca gcagccaact cagcttcctt tcgggctttg ttagcagccg
     4021 gatctcagtg gtggtggtgg tggtgctcga gcggtcagcc aaacgtctct tcaggccact
     4081 gactagcgat aactttcccc acaacggaac aactctcatt gcatgggatc attgggtact
     4141 gtgggtttag tggttgtaaa aacacctgac cgctatccct gatcagtttc ttgaaggtaa
     4201 actcatcacc cccaagtctg gctatgcaga aatcacctgg ctcaacagcc tgctcagggt
     4261 caacgagaat taacattccg tcaggaaagc ttggcttgga gcctgttggt gcggtcatgg
     4321 aattaccttc aacctcaagc cagaatgcag aatcactggc ttttttggtt gtgcttaccc
     4381 atctctccgc atcacctttg gtaaaggttc taagcttagg tgagaacatc cctgcctgaa
     4441 catgagaaaa aacagggtac tcatactcac ttctaagtga cggctgcata ctaaccgctt
     4501 catacatctc gtagatttct ctggcgattg aagggctaaa ttcttcaacg ctaactttga
     4561 gaatttttgt aagcaatgcg gcgttataag catttaatgc attgatgcca ttaaataaag
     4621 caccaacgcc tgactgcccc atccccatct tgtctgcgac agattcctgg gataagccaa
     4681 gttcattttt ctttttttca taaattgctt taaggcgacg tgcgtcctca agctgctctt
     4741 gtgttaatgg tttctttttt gtgctcatac gttaaatcta tcaccgcaag ggataaatat
     4801 ctaacaccgt gcgtgttgac tattttacct ctggcggtga taatggttgc atgtactaag
     4861 gaggtaaaca tatggtacgc tggactttgt gggataccct cgctttcctg ctcctgttga
     4921 gtttattgct gccgtcattg cttattatgt tcatcccgtc aacattcaaa cggcctgtct
     4981 catcatggaa ggcgctgaat ttacggaaaa cattattaat ggcgtcgagc gtccggttaa
     5041 agccgctgaa ttgttcgcgc ttaccttgcg tgtacgcgca ggaaacactg acgttcttgc
     5101 tgacgcagaa gaaaacgtgc gtcaaaaatt acgtgcagaa ggagtgaaag gaaaaaagc
//
