Basic Vector Information
- Vector Name:
- pMENCHU
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4152 bp
- Type:
- Plant expression vector
- Replication origin:
- ori
- Host:
- Plants
- Source/Author:
- Ferrando A, Farras R, Jasik J, Schell J, Koncz C.
- Promoter:
- CaMV 35S
pMENCHU vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMENCHU vector Sequence
LOCUS 40924_30620 4152 bp DNA circular SYN 18-DEC-2018
DEFINITION Plant expression vector pMENCHU, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4152)
AUTHORS Ferrando A, Farras R, Jasik J, Schell J, Koncz C.
TITLE Intron-tagged epitope: a tool for facile detection and purification
of proteins expressed in Agrobacterium-transformed plant cells
JOURNAL Plant J. 22 (6), 553-560 (2000)
PUBMED 10886775
REFERENCE 2 (bases 1 to 4152)
AUTHORS Berendzen KW, Koncz C.
TITLE Direct Submission
JOURNAL Submitted (12-AUG-2004) Development, Max-Planck Institute for Plant
Breeding Research, Carl-von-Linne-Weg 10, Cologne D-50829, Germany
REFERENCE 3 (bases 1 to 4152)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4152)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plant J.";
date: "2000"; volume: "22"; issue: "6"; pages: "553-560"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(12-AUG-2004) Development, Max-Planck Institute for Plant Breeding
Research, Carl-von-Linne-Weg 10, Cologne D-50829, Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4152
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 1..18
/note="Tth111I, NotI, XhoI, KpnI; MCS promoter"
promoter 409..754
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
misc_feature 759..793
/note="HindIII, NcoI, SmaI, XbaI, BglII; MCS upstream"
misc_feature 794..805
/label=N-term HA epitope
/note="N-term HA epitope"
intron 806..994
/label=IV2 intron
/note="modified second intron of the potato ST-LS1 gene
(Vancanneyt et al., 1990)"
misc_feature 995..1009
/label=C-term HA epitope
/note="C-term HA epitope"
misc_feature 1010..1036
/note="SalI, BamHI, PstI, EcoRI; MCS downstream"
regulatory 1037..1289
/note="polyadenylation signal from nopaline synthase gene
of the Agrobacterium Ti plasmid; NOS terminator"
/regulatory_class="terminator"
promoter complement(1321..1339)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1369..1385)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(1393..1409)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1417..1447)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(1462..1483)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(1771..2359)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2533..3390)
/label=AmpR
/note="beta-lactamase"
promoter complement(3391..3495)
/label=AmpR promoter
rep_origin 3522..3977
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 4118..4134
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
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