LOCUS       40924_30620        4152 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Plant expression vector pMENCHU, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Ferrando A, Farras R, Jasik J, Schell J, Koncz C.
  TITLE     Intron-tagged epitope: a tool for facile detection and purification 
            of proteins expressed in Agrobacterium-transformed plant cells
  JOURNAL   Plant J. 22 (6), 553-560 (2000)
  PUBMED    10886775
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Berendzen KW, Koncz C.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-AUG-2004) Development, Max-Planck Institute for Plant 
            Breeding Research, Carl-von-Linne-Weg 10, Cologne D-50829, Germany
REFERENCE   3  (bases 1 to 4152)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4152)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2000"; volume: "22"; issue: "6"; pages: "553-560"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-AUG-2004) Development, Max-Planck Institute for Plant Breeding 
            Research, Carl-von-Linne-Weg 10, Cologne D-50829, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..18
                     /note="Tth111I, NotI, XhoI, KpnI; MCS promoter"
     promoter        409..754
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    759..793
                     /note="HindIII, NcoI, SmaI, XbaI, BglII; MCS upstream"
     misc_feature    794..805
                     /label=N-term HA epitope
                     /note="N-term HA epitope"
     intron          806..994
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     misc_feature    995..1009
                     /label=C-term HA epitope
                     /note="C-term HA epitope"
     misc_feature    1010..1036
                     /note="SalI, BamHI, PstI, EcoRI; MCS downstream"
     regulatory      1037..1289
                     /note="polyadenylation signal from nopaline synthase gene
                     of the Agrobacterium Ti plasmid; NOS terminator"
                     /regulatory_class="terminator"
     promoter        complement(1321..1339)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1369..1385)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1393..1409)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1417..1447)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1462..1483)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1771..2359)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2533..3390)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3391..3495)
                     /label=AmpR promoter
     rep_origin      3522..3977
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     4118..4134
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gcggccgctc gaggtacccc tactccaaaa atgtcaaaga tacagtctca ntaagnacca
       61 aagggctatt gagacttttc aacaaagggt aatttcggga aacctcctcg gattccattg
      121 cccagctatc tgtcacttca tcgaaaggac agtagaaaag gaaggtggct cctacaaatg
      181 ccatcattgc gataaaggaa aggctatcat tcaagatgcc tctgccgaca gtggtcccaa
      241 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      301 aaagcaagtg gattgatgtg acatctccac tgacgtaagg gatgacgcac aatcccaccc
      361 ctactccaaa aatgtcaaag atacagtctc agaagaccaa agggctattg agacttttca
      421 acaaagggta atttcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
      481 cgaaaggaca gtagaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa
      541 ggctatcatt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
      601 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
      661 catctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc
      721 tatataagga agttcatttc atttggagag gacagcccaa gcttccacca tggcgcccgg
      781 gtctagaaga tcttatccat acgatgtaag tttctgcttc tacctttgat atatatataa
      841 taattatcat taattagtag taatataata ttttaaatat ttttttcaaa ataaaagaat
      901 gtagtatata gcaattgctt ttctgtagtt tataagtgtg tatatttcaa tttataactt
      961 ttctaatata tgaccaaaat ttgttgatgt gcaggttcca gattatgctg gtcgacggat
     1021 ccactgcagt gaattctcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
     1081 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
     1141 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
     1201 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
     1261 gcgcggtgtc atctgtcgag ggggggcccg gtaccgagct cgcggccgct cgacgagctc
     1321 cctatagtga gtcgtattan aggccgactt ggccaaattc gtaatcatgt catagctgtt
     1381 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     1441 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     1501 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     1561 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     1621 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     1681 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     1741 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     1801 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     1861 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     1921 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     1981 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     2041 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     2101 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     2161 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     2221 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     2281 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     2341 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     2401 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     2461 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     2521 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     2581 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     2641 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     2701 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     2761 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     2821 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     2881 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     2941 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     3001 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     3061 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     3121 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     3181 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     3241 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     3301 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     3361 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     3421 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     3481 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc
     3541 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3601 agggcccggt cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3661 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3721 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     3781 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     3841 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     3901 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     3961 attaacgctt acaatttcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     4021 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     4081 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag
     4141 ctgacttggt ca
//
