pME9 vector (V004771)

LOCUS       40924_30580        7485 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pME9, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Audtho M, Ratlertkarn M, Promdonkoy B.
  TITLE     pME9, a new vector for sporulation stage expression in Bacillus spp.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7485)
  AUTHORS   Audtho M, Ratlertkarn M, Promdonkoy B.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2013) National Center for Genetic Engineering and 
            Biotechnology, National Science and Technology Development Agency, 
            113 Phaholyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120,
            Thailand
REFERENCE   3  (bases 1 to 7485)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7485)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-FEB-2013) National Center for Genetic Engineering and 
            Biotechnology, National Science and Technology Development Agency, 
            113 Phaholyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120,
            Thailand"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    13..18
                     /label=CTGCAG recognition site for PstI
                     /note="CTGCAG recognition site for PstI"
     regulatory      18..270
                     /label=from Bacillus thuringiensis cyt2Aa gene
                     /note="from Bacillus thuringiensis cyt2Aa gene"
                     /regulatory_class="promoter"
     misc_feature    269..355
                     /note="contains cloning sites EcoRI, EcoRV, HindIII, BglII,
                     SalI, NotI, SacI, SacII, PstI, XbaI, XhoI, ApaI, KpnI"
     promoter        complement(368..386)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(407..423)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(431..447)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(455..485)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(500..521)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(809..1397)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1571..2428)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2429..2533)
                     /label=AmpR promoter
     rep_origin      2560..3015
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3141..3153
                     /label=M13 forward primer
                     /note="M13 forward primer"
     primer_bind     3156..3172
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3179..3197
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_difference 3539
                     /replace="g"
                     /note="G > C to delete EcoRI site of DNA fragment from
                     pBC16"
     CDS             4057..5058
                     /label=repB
                     /note="RepB replication protein"
     CDS             5261..6634
                     /label=TcR
                     /note="tetracycline efflux protein"
     misc_difference 6695
                     /replace="g"
                     /note="G > C to delete EcoRI site in DNA fragment from
                     pBC16"
     rep_origin      6754..7030
                     /label=ori L
                     /note="ori L"
     misc_feature    7201..7485
                     /label=from mob gene
                     /note="from mob gene"