pME9 vector (V004771)

MSDS

Basic Vector Information

Vector Name:: pME9

Antibiotic Resistance:: Ampicillin

Length:: 7485 bp

Type:: Expression vector

Replication origin:: ori

Source/Author:: Audtho M, Ratlertkarn M, Promdonkoy B.

Promoter:: T3

pME9 vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pME9 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_30580        7485 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pME9, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Audtho M, Ratlertkarn M, Promdonkoy B.
  TITLE     pME9, a new vector for sporulation stage expression in Bacillus spp.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7485)
  AUTHORS   Audtho M, Ratlertkarn M, Promdonkoy B.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2013) National Center for Genetic Engineering and 
            Biotechnology, National Science and Technology Development Agency, 
            113 Phaholyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120,
            Thailand
REFERENCE   3  (bases 1 to 7485)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7485)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-FEB-2013) National Center for Genetic Engineering and 
            Biotechnology, National Science and Technology Development Agency, 
            113 Phaholyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120,
            Thailand"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    13..18
                     /label=CTGCAG recognition site for PstI
                     /note="CTGCAG recognition site for PstI"
     regulatory      18..270
                     /label=from Bacillus thuringiensis cyt2Aa gene
                     /note="from Bacillus thuringiensis cyt2Aa gene"
                     /regulatory_class="promoter"
     misc_feature    269..355
                     /note="contains cloning sites EcoRI, EcoRV, HindIII, BglII,
                     SalI, NotI, SacI, SacII, PstI, XbaI, XhoI, ApaI, KpnI"
     promoter        complement(368..386)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(407..423)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(431..447)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(455..485)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(500..521)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(809..1397)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1571..2428)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2429..2533)
                     /label=AmpR promoter
     rep_origin      2560..3015
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3141..3153
                     /label=M13 forward primer
                     /note="M13 forward primer"
     primer_bind     3156..3172
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3179..3197
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_difference 3539
                     /replace="g"
                     /note="G > C to delete EcoRI site of DNA fragment from
                     pBC16"
     CDS             4057..5058
                     /label=repB
                     /note="RepB replication protein"
     CDS             5261..6634
                     /label=TcR
                     /note="tetracycline efflux protein"
     misc_difference 6695
                     /replace="g"
                     /note="G > C to delete EcoRI site in DNA fragment from
                     pBC16"
     rep_origin      6754..7030
                     /label=ori L
                     /note="ori L"
     misc_feature    7201..7485
                     /label=from mob gene
                     /note="from mob gene"

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