LOCUS       40924_30580        7485 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pME9, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7485)
  AUTHORS   Audtho M, Ratlertkarn M, Promdonkoy B.
  TITLE     pME9, a new vector for sporulation stage expression in Bacillus spp.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7485)
  AUTHORS   Audtho M, Ratlertkarn M, Promdonkoy B.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2013) National Center for Genetic Engineering and 
            Biotechnology, National Science and Technology Development Agency, 
            113 Phaholyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120,
            Thailand
REFERENCE   3  (bases 1 to 7485)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7485)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-FEB-2013) National Center for Genetic Engineering and 
            Biotechnology, National Science and Technology Development Agency, 
            113 Phaholyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120,
            Thailand"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7485
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    13..18
                     /label=CTGCAG recognition site for PstI
                     /note="CTGCAG recognition site for PstI"
     regulatory      18..270
                     /label=from Bacillus thuringiensis cyt2Aa gene
                     /note="from Bacillus thuringiensis cyt2Aa gene"
                     /regulatory_class="promoter"
     misc_feature    269..355
                     /note="contains cloning sites EcoRI, EcoRV, HindIII, BglII,
                     SalI, NotI, SacI, SacII, PstI, XbaI, XhoI, ApaI, KpnI"
     promoter        complement(368..386)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(407..423)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(431..447)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(455..485)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(500..521)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(809..1397)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1571..2428)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2429..2533)
                     /label=AmpR promoter
     rep_origin      2560..3015
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3141..3153
                     /label=M13 forward primer
                     /note="M13 forward primer"
     primer_bind     3156..3172
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3179..3197
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_difference 3539
                     /replace="g"
                     /note="G > C to delete EcoRI site of DNA fragment from
                     pBC16"
     CDS             4057..5058
                     /label=repB
                     /note="RepB replication protein"
     CDS             5261..6634
                     /label=TcR
                     /note="tetracycline efflux protein"
     misc_difference 6695
                     /replace="g"
                     /note="G > C to delete EcoRI site in DNA fragment from
                     pBC16"
     rep_origin      6754..7030
                     /label=ori L
                     /note="ori L"
     misc_feature    7201..7485
                     /label=from mob gene
                     /note="from mob gene"
ORIGIN
        1 cgatcccccg ggctgcaggt aactaagttt aaggaacttt gcaacaaaac caataatcat
       61 atcaggatgt aacaaaaaag attcatacaa tcctcttatt tttatatgga gtacatattt
      121 tatcaccgcc tgaatactag agaataagaa agtctatttt ggaatgagga ataattataa
      181 ttcaataaaa atatgtataa tgttgtaagt taaaaaaatg tagaattgta ataaatctta
      241 aattttaata aatggtaatt aaaaggagga attcgatatc aagcttagat ctaccgtcga
      301 cgcggccgcg agctcccgcg gctgcagtct agacctcgag ggggggcccg gtacccagct
      361 tttgttccct ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc
      421 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
      481 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      541 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
      601 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
      661 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      721 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      781 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      841 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      901 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      961 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1021 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1081 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1141 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1201 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     1261 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1321 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1381 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1441 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1501 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1561 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     1621 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     1681 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     1741 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     1801 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     1861 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     1921 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     1981 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2041 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2101 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     2161 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     2221 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     2281 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     2341 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     2401 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     2461 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     2521 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt agcggcgcat
     2581 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     2641 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     2701 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     2761 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     2821 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     2881 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     2941 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     3001 taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     3061 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     3121 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattgta
     3181 atacgactca ctatagggcg actagtgaat ggactgctta tagcgataaa gttaaatcag
     3241 atttagaagt accagcgaaa cgacacatga aaagtgttga agtgccaacg ggtgaaaagt
     3301 ccatgtttgg tttgggaaaa gaaataatga aaacagaaaa gaaaccaacc aaaaatgttg
     3361 ttatatcgga gcgtgattat aaaaacttag tgactgctgc gagagataac gataggttaa
     3421 aacagcatgt tagaaatctc atgagtactg atatggcgag agaatataaa aaattaagta
     3481 aagaacatgg gcaagttaaa gaaaaatata gtggtcttgt agagcgattt aatgaaaatg
     3541 taaatgatta taatgagttg cttgaagaaa acaagtcttt aaagtctaaa ataagcgatt
     3601 taaagcgtga tgtgagttta atctatgaaa gcactaagca attccttaag gaacgtacag
     3661 acggcttaaa agcctttaaa aacgttttta aggggtttgt agacaaggta aaggataaaa
     3721 cagcacaatt ccaagaaaaa cacgatttag aacctaaaaa gaacgaattt gaactaactc
     3781 ataaccgaga ggtaaaaaaa gaacgaagtc gagatcaggg aatgagttta taaaataaaa
     3841 aaagcacctg aaaaggtgtc tttttttgat ggttttgaac ttgttctttc ttatcttgat
     3901 acatatagaa ataacgtcat ttttatttta gttgctgaaa ggtgcgttga agtgttggta
     3961 tgtatgtgtt ttaaagtatt gaaaaccctt aaaattggtt gcacagaaaa accccatctg
     4021 ttaaagttat aagtgaccaa acaaataact aaatagatgg gggtttcttt taatattatg
     4081 tgtcctaata gtagcattta ttcagatgaa aaatcaaggg ttttagtgga caagacaaaa
     4141 agtggaaaag tgagaccatg gagagaaaag aaaatcgcta atgttgatta ctttgaactt
     4201 ctgcatattc ttgaatttaa aaaggctgaa agagtaaaag attgtgctga aatattagag
     4261 tataaacaaa atcgtgaaac aggcgaaaga aagttgtatc gagtgtggtt ttgtaaatcc
     4321 aggctttgtc caatgtgcaa ctggaggaga gcaatgaaac atggcattca gtcacaaaag
     4381 gttgttgctg aagttattaa acaaaagcca acagttcgtt ggttgtttct cacattaaca
     4441 gttaaaaatg tttatgatgg cgaagaatta aataagagtt tgtcagatat ggctcaagga
     4501 tttcgccgaa tgatgcaata taaaaaaatt aataaaaatc ttgttggttt tatgcgtgca
     4561 acggaagtga caataaataa taaagataat tcttataatc agcacatgca tgtattggta
     4621 tgtgtggaac caacttattt taagaataca gaaaactacg tgaatcaaaa acaatggatt
     4681 caattttgga aaaaggcaat gaaattagac tatgatccaa atgtaaaagt tcaaatgatt
     4741 cgaccgaaaa ataaatataa atcggatata caatcggcaa ttgacgaaac tgcaaaatat
     4801 cctgtaaagg atacggattt tatgaccgat gatgaagaaa agaatttgaa acgtttgtct
     4861 gatttggagg aaggtttaca ccgtaaaagg ttaatctcct atggtggttt gttaaaagaa
     4921 atacataaaa aattaaacct tgatgacaca gaagaaggcg atttgattca tacagatgat
     4981 gacgaaaaag ccgatgaaga tggattttct attattgcaa tgtggaattg ggaacggaaa
     5041 aattatttta ttaaagagta gttcaacaaa cgggccatat tgttgtataa gtgatgaaat
     5101 actgaattta aaacttagtt tatatgtggt aaaatgtttt aatcaagttt aggaggaatt
     5161 aattatgaag tgtaatgaat gtaacagggt tcaattaaaa gagggaagcg tatcattaac
     5221 cctataaact acgtctgccc tcattattgg agggtgacat atgaatacat cctattcaca
     5281 atcgaattta cgacacaacc aaattttaat ttggctttgc attttatctt tttttagcgt
     5341 attaaatgaa atggttttga acgtctcatt acctgatatt gcaaatgatt ttaataaacc
     5401 acctgcgagt acaaactggg tgaacacagc ctttatgtta accttttcca ttggaacagc
     5461 tgtatatgga aagctatctg atcaattagg catcaaaagg ttactcctat ttggaattat
     5521 aataaattgt ttcgggtcgg taattgggtt tgttggccat tctttctttt ccttacttat
     5581 tatggctcgt tttattcaag gggctggtgc agctgcattt ccagcactcg taatggttgt
     5641 agttgcgcgc tatattccaa aggaaaatag gggtaaagca tttggtctta ttggatcgat
     5701 agtagccatg ggagaaggag tcggtccagc gattggtgga atgatagccc attatattca
     5761 ttggtcctat cttctactca ttcctatgat aacaattatc actgttccgt ttcttatgaa
     5821 attattaaag aaagaagtaa ggataaaagg tcattttgat atcaaaggaa ttatactaat
     5881 gtctgtaggc attgtatttt ttatgttgtt tacaacatca tatagcattt cttttcttat
     5941 cgttagcgtg ctgtcattcc tgatatttgt aaaacatatc aggaaagtaa cagatccttt
     6001 tgttgatccc ggattaggga aaaatatacc ttttatgatt ggagttcttt gtgggggaat
     6061 tatatttgga acagtagcag ggtttgtctc tatggttcct tatatgatga aagatgttca
     6121 ccagctaagt actgccgaaa tcggaagtgt aattattttc cctggaacaa tgagtgtcat
     6181 tattttcggc tacattggtg ggatacttgt tgatagaaga ggtcctttat acgtgttaaa
     6241 catcggagtt acatttcttt ctgttagctt tttaactgct tcctttcttt tagaaacaac
     6301 atcatggttc atgacaatta taatcgtatt tgttttaggt gggctttcgt tcaccaaaac
     6361 agttatatca acaattgttt caagtagctt gaaacagcag gaagctggtg ctggaatgag
     6421 tttgcttaac tttaccagct ttttatcaga gggaacaggt attgcaattg taggtggttt
     6481 attatccata cccttacttg atcaaaggtt gttacctatg gaagttgatc agtcaactta
     6541 tctgtatagt aatttgttat tacttttttc aggaatcatt gtcattagtt ggctggttac
     6601 cttgaatgta tataaacatt ctcaaaggga tttctaaatc gttaagggat caactttggg
     6661 agagagttca aaattgatcc tttttttata acagcaattc aaatcttttt gttccattaa
     6721 agggcgcgat tgctgaataa aagatacgag agacctctct tgtatctttt ttattttgag
     6781 tggttttgtc cgttacacta gaaaaccgaa agacaataaa aattttattc ttgctgagtc
     6841 tggctttcgg taagctagac aaaacggaca aaataaaaat tggcaagggt ttaaaggtgg
     6901 agattttttg agtgatcttc tcaaaaaata ctacctgtcc cttgctgatt tttaaacgag
     6961 cacgagagca aaacccccct ttgctgaggt ggcagagggc aggttttttt gtttcttttt
     7021 tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt tggtcggcac tgccgacagc
     7081 ctcgcagagc acacacttta tgaatataaa gtatagtgtg ttatacttta cttggaagtg
     7141 gttgccggaa agagcgaaaa tgcctcacat ttgtgccacc taaaaaggag cgatttacat
     7201 atgagttatg cagtttgtag aatgcaaaaa gtgaaatcag ctggactaaa aggcatgcaa
     7261 tttcataatc aaagagagcg aaaaagtaga acgaatgatg atattgacca tgagcgaaca
     7321 cgtgaaaatt atgatttgaa aaatgataaa aatattgatt acaacgaacg tgtcaaagaa
     7381 attattgaat cacaaaaaac aggtacaaga aaaacgagga aagatgctgt tcttgtaaat
     7441 gagttgctag taacatctga ccgagatttt tttgagcaac tggat
//
