pME10 vector (V004783)

LOCUS       40924_30515        7850 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pME10, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7850)
  AUTHORS   Audtho M, Maureen K, Ratlertkarn M, Rattanalapho C.
  TITLE     pME13, an improved expression vector for vegetative stage of 
            Bacillus spp.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7850)
  AUTHORS   Audtho M, Maureen K, Ratlertkarn M, Rattanalapho C.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2015) National Center for Genetic Engineering and 
            Biotechnology, National Science and Technology Development Agency, 
            113 Phaholyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120,
            Thailand
REFERENCE   3  (bases 1 to 7850)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7850)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-JUL-2015) National Center for Genetic Engineering and 
            Biotechnology, National Science and Technology Development Agency, 
            113 Phaholyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120,
            Thailand"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      25..652
                     /label=from Bacillus thuringiensis subsp. tenebrionis
                     /note="from Bacillus thuringiensis subsp. tenebrionis"
                     /regulatory_class="promoter"
     misc_feature    652..719
                     /note="multiple cloning site; 
                     BglII-SalI-NotI-SacI-SacII-PstI-XbaI-XhoI-ApaI-KpnI"
     promoter        complement(733..751)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(772..788)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(796..812)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(820..850)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(865..886)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1174..1762)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1936..2793)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2794..2898)
                     /label=AmpR promoter
     rep_origin      2925..3380
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3521..3537
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3544..3562
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3571..4198
                     /label=mob fragment
                     /note="mob fragment"
     CDS             4422..5423
                     /label=repB
                     /note="RepB replication protein"
     CDS             5626..6999
                     /label=TcR
                     /note="tetracycline efflux protein"
     rep_origin      7119..7395
                     /label=ori L
                     /note="ori L"
     misc_feature    7566..7850
                     /label=mob fragment
                     /note="mob fragment"