LOCUS       40924_30515        7850 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pME10, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7850)
  AUTHORS   Audtho M, Maureen K, Ratlertkarn M, Rattanalapho C.
  TITLE     pME13, an improved expression vector for vegetative stage of 
            Bacillus spp.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7850)
  AUTHORS   Audtho M, Maureen K, Ratlertkarn M, Rattanalapho C.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-JUL-2015) National Center for Genetic Engineering and 
            Biotechnology, National Science and Technology Development Agency, 
            113 Phaholyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120,
            Thailand
REFERENCE   3  (bases 1 to 7850)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7850)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-JUL-2015) National Center for Genetic Engineering and 
            Biotechnology, National Science and Technology Development Agency, 
            113 Phaholyothin Road, Klong Nueng, Klong Luang, Pathum Thani 12120,
            Thailand"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..7850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      25..652
                     /label=from Bacillus thuringiensis subsp. tenebrionis
                     /note="from Bacillus thuringiensis subsp. tenebrionis"
                     /regulatory_class="promoter"
     misc_feature    652..719
                     /note="multiple cloning site; 
                     BglII-SalI-NotI-SacI-SacII-PstI-XbaI-XhoI-ApaI-KpnI"
     promoter        complement(733..751)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(772..788)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(796..812)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(820..850)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(865..886)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1174..1762)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1936..2793)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2794..2898)
                     /label=AmpR promoter
     rep_origin      2925..3380
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     3521..3537
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3544..3562
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    3571..4198
                     /label=mob fragment
                     /note="mob fragment"
     CDS             4422..5423
                     /label=repB
                     /note="RepB replication protein"
     CDS             5626..6999
                     /label=TcR
                     /note="tetracycline efflux protein"
     rep_origin      7119..7395
                     /label=ori L
                     /note="ori L"
     misc_feature    7566..7850
                     /label=mob fragment
                     /note="mob fragment"
ORIGIN
        1 cgatcccccg ggctgcagga attcgaaacg taagatgaaa ccttagataa aagtgctttt
       61 tttgttgcaa ttgaagaatt attaatgtta agcttaatta aagataatat ctttgaattg
      121 taacgcccct caaaagtaag aactacaaaa aaagaatacg ttatatagaa atatgtttga
      181 accttcttca gattacaaat atattcggac ggactctacc tcaaatgctt atctaactat
      241 agaatgacat acaagcacaa ccttgaaaat ttgaaaatat aactaccaat gaacttgttc
      301 atgtgaatta tcgctgtatt taattttctc aattcaatat ataatatgcc aatacattgt
      361 tacaagtaga aattaagaca cccttgatag ccttactata cctaacatga tgtagtatta
      421 aatgaatatg taaatatatt tatgataaga agcgacttat ttataatcat tacatatttt
      481 tctattggaa tgattaagat tccaatagaa tagtgtataa attatttatc ttgaaaggag
      541 ggatgcctaa aaacgaagaa cattaaaaac atatatttgc accgtctaat ggatttatga
      601 aaaatcattt tatcagtttg aaaattatgt attatgataa gaaagggagg aagatctacc
      661 gtcgacgcgg ccgcgagctc ccgcggctgc agtctagacc tcgagggggg gcccggtacc
      721 cagcttttgt tccctttagt gagggttaat ttcgagcttg gcgtaatcat ggtcatagct
      781 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      841 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      901 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
      961 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     1021 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     1081 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     1141 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     1201 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     1261 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     1321 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     1381 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     1441 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     1501 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     1561 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     1621 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     1681 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     1741 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     1801 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     1861 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1921 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     1981 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     2041 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     2101 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     2161 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     2221 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     2281 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     2341 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     2401 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     2461 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     2521 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     2581 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     2641 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     2701 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     2761 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     2821 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     2881 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg
     2941 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     3001 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     3061 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     3121 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     3181 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     3241 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     3301 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     3361 aatattaacg cttacaattt ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     3421 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     3481 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     3541 ttgtaatacg actcactata gggcgactag tgaatggact gcttatagcg ataaagttaa
     3601 atcagattta gaagtaccag cgaaacgaca catgaaaagt gttgaagtgc caacgggtga
     3661 aaagtccatg tttggtttgg gaaaagaaat aatgaaaaca gaaaagaaac caaccaaaaa
     3721 tgttgttata tcggagcgtg attataaaaa cttagtgact gctgcgagag ataacgatag
     3781 gttaaaacag catgttagaa atctcatgag tactgatatg gcgagagaat ataaaaaatt
     3841 aagtaaagaa catgggcaag ttaaagaaaa atatagtggt cttgtagagc gatttaatga
     3901 aaatgtaaat gattataatg agttgcttga agaaaacaag tctttaaagt ctaaaataag
     3961 cgatttaaag cgtgatgtga gtttaatcta tgaaagcact aagcaattcc ttaaggaacg
     4021 tacagacggc ttaaaagcct ttaaaaacgt ttttaagggg tttgtagaca aggtaaagga
     4081 taaaacagca caattccaag aaaaacacga tttagaacct aaaaagaacg aatttgaact
     4141 aactcataac cgagaggtaa aaaaagaacg aagtcgagat cagggaatga gtttataaaa
     4201 taaaaaaagc acctgaaaag gtgtcttttt ttgatggttt tgaacttgtt ctttcttatc
     4261 ttgatacata tagaaataac gtcattttta ttttagttgc tgaaaggtgc gttgaagtgt
     4321 tggtatgtat gtgttttaaa gtattgaaaa cccttaaaat tggttgcaca gaaaaacccc
     4381 atctgttaaa gttataagtg accaaacaaa taactaaata gatgggggtt tcttttaata
     4441 ttatgtgtcc taatagtagc atttattcag atgaaaaatc aagggtttta gtggacaaga
     4501 caaaaagtgg aaaagtgaga ccatggagag aaaagaaaat cgctaatgtt gattactttg
     4561 aacttctgca tattcttgaa tttaaaaagg ctgaaagagt aaaagattgt gctgaaatat
     4621 tagagtataa acaaaatcgt gaaacaggcg aaagaaagtt gtatcgagtg tggttttgta
     4681 aatccaggct ttgtccaatg tgcaactgga ggagagcaat gaaacatggc attcagtcac
     4741 aaaaggttgt tgctgaagtt attaaacaaa agccaacagt tcgttggttg tttctcacat
     4801 taacagttaa aaatgtttat gatggcgaag aattaaataa gagtttgtca gatatggctc
     4861 aaggatttcg ccgaatgatg caatataaaa aaattaataa aaatcttgtt ggttttatgc
     4921 gtgcaacgga agtgacaata aataataaag ataattctta taatcagcac atgcatgtat
     4981 tggtatgtgt ggaaccaact tattttaaga atacagaaaa ctacgtgaat caaaaacaat
     5041 ggattcaatt ttggaaaaag gcaatgaaat tagactatga tccaaatgta aaagttcaaa
     5101 tgattcgacc gaaaaataaa tataaatcgg atatacaatc ggcaattgac gaaactgcaa
     5161 aatatcctgt aaaggatacg gattttatga ccgatgatga agaaaagaat ttgaaacgtt
     5221 tgtctgattt ggaggaaggt ttacaccgta aaaggttaat ctcctatggt ggtttgttaa
     5281 aagaaataca taaaaaatta aaccttgatg acacagaaga aggcgatttg attcatacag
     5341 atgatgacga aaaagccgat gaagatggat tttctattat tgcaatgtgg aattgggaac
     5401 ggaaaaatta ttttattaaa gagtagttca acaaacgggc catattgttg tataagtgat
     5461 gaaatactga atttaaaact tagtttatat gtggtaaaat gttttaatca agtttaggag
     5521 gaattaatta tgaagtgtaa tgaatgtaac agggttcaat taaaagaggg aagcgtatca
     5581 ttaaccctat aaactacgtc tgccctcatt attggagggt gacatatgaa tacatcctat
     5641 tcacaatcga atttacgaca caaccaaatt ttaatttggc tttgcatttt atcttttttt
     5701 agcgtattaa atgaaatggt tttgaacgtc tcattacctg atattgcaaa tgattttaat
     5761 aaaccacctg cgagtacaaa ctgggtgaac acagccttta tgttaacctt ttccattgga
     5821 acagctgtat atggaaagct atctgatcaa ttaggcatca aaaggttact cctatttgga
     5881 attataataa attgtttcgg gtcggtaatt gggtttgttg gccattcttt cttttcctta
     5941 cttattatgg ctcgttttat tcaaggggct ggtgcagctg catttccagc actcgtaatg
     6001 gttgtagttg cgcgctatat tccaaaggaa aataggggta aagcatttgg tcttattgga
     6061 tcgatagtag ccatgggaga aggagtcggt ccagcgattg gtggaatgat agcccattat
     6121 attcattggt cctatcttct actcattcct atgataacaa ttatcactgt tccgtttctt
     6181 atgaaattat taaagaaaga agtaaggata aaaggtcatt ttgatatcaa aggaattata
     6241 ctaatgtctg taggcattgt attttttatg ttgtttacaa catcatatag catttctttt
     6301 cttatcgtta gcgtgctgtc attcctgata tttgtaaaac atatcaggaa agtaacagat
     6361 ccttttgttg atcccggatt agggaaaaat atacctttta tgattggagt tctttgtggg
     6421 ggaattatat ttggaacagt agcagggttt gtctctatgg ttccttatat gatgaaagat
     6481 gttcaccagc taagtactgc cgaaatcgga agtgtaatta ttttccctgg aacaatgagt
     6541 gtcattattt tcggctacat tggtgggata cttgttgata gaagaggtcc tttatacgtg
     6601 ttaaacatcg gagttacatt tctttctgtt agctttttaa ctgcttcctt tcttttagaa
     6661 acaacatcat ggttcatgac aattataatc gtatttgttt taggtgggct ttcgttcacc
     6721 aaaacagtta tatcaacaat tgtttcaagt agcttgaaac agcaggaagc tggtgctgga
     6781 atgagtttgc ttaactttac cagcttttta tcagagggaa caggtattgc aattgtaggt
     6841 ggtttattat ccataccctt acttgatcaa aggttgttac ctatggaagt tgatcagtca
     6901 acttatctgt atagtaattt gttattactt ttttcaggaa tcattgtcat tagttggctg
     6961 gttaccttga atgtatataa acattctcaa agggatttct aaatcgttaa gggatcaact
     7021 ttgggagaga gttcaaaatt gatccttttt ttataacagc aattcaaatc tttttgttcc
     7081 attaaagggc gcgattgctg aataaaagat acgagagacc tctcttgtat cttttttatt
     7141 ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt tattcttgct
     7201 gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca agggtttaaa
     7261 ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc tgatttttaa
     7321 acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt tttttgtttc
     7381 ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc ggcactgccg
     7441 acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata ctttacttgg
     7501 aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt
     7561 tacatatgag ttatgcagtt tgtagaatgc aaaaagtgaa atcagctgga ctaaaaggca
     7621 tgcaatttca taatcaaaga gagcgaaaaa gtagaacgaa tgatgatatt gaccatgagc
     7681 gaacacgtga aaattatgat ttgaaaaatg ataaaaatat tgattacaac gaacgtgtca
     7741 aagaaattat tgaatcacaa aaaacaggta caagaaaaac gaggaaagat gctgttcttg
     7801 taaatgagtt gctagtaaca tctgaccgag atttttttga gcaactggat
//
