pME-lama4-NS vector (V004787)

LOCUS       40924_30495        8158 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pME-lama4-NS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Sztal TE, Sonntag C, Hall TE, Currie PD.
  TITLE     Epistatic dissection of laminin-receptor interactions in dystrophic 
            zebrafish muscle
  JOURNAL   Hum. Mol. Genet. 21 (21), 4718-4731 (2012)
  PUBMED    22859503
REFERENCE   2  (bases 1 to 8158)
  AUTHORS   Hall TE, Sztal TE, Currie PD.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2012) Institute for Molecular Bioscience, 
            University of Queensland, 306 Carmody Road, St Lucia, QLD 4067, 
            Australia
REFERENCE   3  (bases 1 to 8158)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8158)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum. Mol. 
            Genet."; date: "2012"; volume: "21"; issue: "21"; pages: "4718-4731"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-JUN-2012) Institute for Molecular Bioscience, University of 
            Queensland, 306 Carmody Road, St Lucia, QLD 4067, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    669..6281
                     /label=similar to laminin alpha4 chain
                     /note="similar to laminin alpha4 chain"
     protein_bind    complement(6283..6382)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(6400..6418)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6423..6439)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6552..7358
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      7508..8096
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"