LOCUS       40924_30495        8158 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pME-lama4-NS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Sztal TE, Sonntag C, Hall TE, Currie PD.
  TITLE     Epistatic dissection of laminin-receptor interactions in dystrophic 
            zebrafish muscle
  JOURNAL   Hum. Mol. Genet. 21 (21), 4718-4731 (2012)
  PUBMED    22859503
REFERENCE   2  (bases 1 to 8158)
  AUTHORS   Hall TE, Sztal TE, Currie PD.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUN-2012) Institute for Molecular Bioscience, 
            University of Queensland, 306 Carmody Road, St Lucia, QLD 4067, 
            Australia
REFERENCE   3  (bases 1 to 8158)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8158)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum. Mol. 
            Genet."; date: "2012"; volume: "21"; issue: "21"; pages: "4718-4731"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-JUN-2012) Institute for Molecular Bioscience, University of 
            Queensland, 306 Carmody Road, St Lucia, QLD 4067, Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8158
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /label=attL1
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    669..6281
                     /label=similar to laminin alpha4 chain
                     /note="similar to laminin alpha4 chain"
     protein_bind    complement(6283..6382)
                     /label=attL2
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(6400..6418)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6423..6439)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6552..7358
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      7508..8096
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctat ggcctctctg ctgtctggat ctgtctgctt tctgtatgtc ttgtcagcac
      721 tcgtgcccgc agattcaaca caacagttca acacagtcta cagggtgtgt gctcgagggt
      781 acttcttgag tgatcaggag acatgtctgc cctgtaactg taaaggccat gcggatagct
      841 gtgaggacat caccgggatc tgtattgatt gtcgggacca cactcttgga gatttctgtg
      901 agcagtgtga agacagattt ttgcccgtgt ctcttcctga tggcaccttt atgtgtagac
      961 cgtgtgcctg cccgctgtca ctggagtcta acaactttgc agctcactgt gataagaggg
     1021 gtgagtttct gagatgtgtc tgccaggacg ggtatgctgg gcactactgt gagagatgtg
     1081 ctccgggtta ttatggcaac cccatggagt tgggcaattc ttgcaaaaag tgtgattgta
     1141 acggcaactc tgaccccaac ctgatcttca acgagtgcca taatgtgaca ggaaagtgcc
     1201 tgaactgctg gggtgacact gcaggggaca actgtgaaag gtgtgcacct ggtttctatg
     1261 gcgacgccat cagtgctaaa gactgtcaag agtgccagtg caataagtgt ggtactgcat
     1321 catgtgatga caggtcaggt gtgtgccact gtaagcctgg ggtcacaggt cagctgtgtg
     1381 acaggtgtga ggacggtcat tctggatttc gcacctgcat gggttgccgt cgatgtgaat
     1441 gtgcttctgc tgccctgcgt gccacctgcc accctctaac acacagctgc cagtgccgcc
     1501 cgggggctgg aggtcgatac tgcgagcgct gcctgccggg attctgggat tacagcccat
     1561 ccggctgcag gaaatgcgac tgtccagaag agaactgtga cctgcacaca ggagagtgtc
     1621 tgcctgagct ctcgcaaatc agcgagtgta atatcagctg tgacgcgtgt atctggaatc
     1681 tgatcggaga cattcgccag tccaacaaaa ctctggacca ggtgaggaac agcgtgctga
     1741 acatttccac aggagctgca gctaatgaca gactgaagta ctacaactac actgcgcacc
     1801 ggctacaggc tcagtttagg ggctggagaa agaattatgc catgatgagt ggagatacag
     1861 atgtcctgga agaagaaact caaaatctgc tgaccgacat ggaccagctc gcagaagagg
     1921 agaatgaagt gcagaatttg ggaaaccaag tggatcaaga gacactgaac agctccacac
     1981 gagcaaagac cctcactgct aacctgtcat cgctcaatgt cctcattgaa gatatgatca
     2041 gggactggga attatacggt gtctatcagg agttggatcc tcaagtgaag ttgcagaaac
     2101 tggatgaagc tgaacggatc ttgggctgga tgagaaaact gaacctgtca cccaaagaac
     2161 cgcttgctac agatgagtcc tccgaggcgc atgagttgct cagacgggtg cgcagctttg
     2221 acagaaatct gatgactaca cagggccgtc tgccacctat gcaggaagtt ctgggtcggt
     2281 ttacttctaa actccttcaa gctcaggatc tccttagcag agctgaagac gcactgaaac
     2341 aggccatcca caaatacaag agcaaccagc tcaaactaca gaggagagag gcacagcagc
     2401 aacgtctgaa agacatccag gacggcgtcg atcaaacaat tgttgatgct acggcagttc
     2461 tctctgaggc aaatcacact gttcttgagg tggaagacgt gatcacgaat gtgagagaac
     2521 agcatgcaga aattgacgga gcccgtgtct tactggagag aaggacagag aagatatctc
     2581 agtcagacag agaactagtt ctgcgagccg aggatcatgc cgatcaactg cagagacacg
     2641 ctgaagagct gcagcataat ctgaaaggaa gcgatgcgaa cggacatgtg cagaaagccc
     2701 tgaacgcctt caacgtgtac gacaatattg tcaagagcgt agacgatgct aacatcacta
     2761 gcctgaccgc cctcaacatc tccagcagga ctcacggtgc ggtcgataag ctaagctctc
     2821 agctggacct catgcgctct cagagtgcaa aagtgttgaa tgaagcatcg tctctacact
     2881 ttcagcagca agacagtgaa tctatcgtac tcgacaataa gaaatacatt gaagagacca
     2941 atgaaatgat ggacggctcc tctaaaaaaa tagctaagat tatcacggag atcaatgcaa
     3001 ttcacacaga tcgtaccccg aagcgtctgc agttcactca ggaagtggct gagggcactc
     3061 tgaaccgctc cgctgaggtg ttggagaaag tgactcccat tctgaagaga gtggagcagt
     3121 gggacggcaa catgaagagt gaagcctatt ctgctgcagc gttcgaccgc actgttctgt
     3181 ctgctggcga ggcggtgaag gacctgtcag ccattgtgcc agagctgctc agcaagctgc
     3241 gtgtggttga ggagaaaaaa cctgtgaata acgtcaccac gaacatcatg aaaatcagag
     3301 agctcatcgc acaggcccgc agcgttgcaa agaaggtgca agtgtccatg aagtttgatg
     3361 gccagtctgc tgtagaagtt catccccaca ccaacctgga cgagctgaag acggtgacgt
     3421 ccattagttt ctacatccga gtagacccag ataaagaccc catagaggat cgcttcttgc
     3481 tttacctcgg agacaaacaa ggaaagaggg actacatggg actcgccata aagaacgata
     3541 atctggtgta cgtctacaaa ctgggtgaag aggaagtgga gattccactc agctccaagc
     3601 cagtcagctc ctggcctcct gtcttcaacc ttgttaaagt ggagaggctg ggacgccatg
     3661 gcaaagtgtt cctgacggtt cccagtcaag acaccacagc cgagcagaag ttcatccaga
     3721 aaggagagcc agacgggaca gactcgctgt ttgacctgga ccctaaagac atggtgttca
     3781 tagttggagg agtgcctcct ggtgtcacgg ttcctccgat tttgagcctg gctcctttcg
     3841 ttggctgcat tgagttagag accctgaata atgatgtcat cagtttgtac aactttaagc
     3901 aacttcacaa aatggatgtt aaggcatcgg tgccttgtcc caggcacaag ctggctttct
     3961 ctcagagtcg tgtcaccagt tatttgtttg acggcaccgg ctacgcttta gtcaacaaca
     4021 ttgagaggcg aggaaagatt ggcgtcgtca ctcgatttga tattgaagtt cgaactgttg
     4081 ctaacaacgg catcctcttc ctcatggtga atgagaccaa tttctttgtg ctggagctga
     4141 aaaatggctt tctccgtctg atgtacgact ttggttttgc taacggtcct gtcatcatag
     4201 agactaactt agccaaactg cagatcaatg acgctcgcta tcacgaggtg tctgtgatct
     4261 atcatcattc aaagaagatc attttgttgg tggacagaag ccatgtcaag tcctttgaaa
     4321 gtgaaaagaa acccctaccc ttttctgata tctatatagg aggagctcct tcgagaattc
     4381 tgcagtctag gccagagctg acatccttga ttggattgaa gggttgtgta aaaggcttcc
     4441 agttccagaa aaaggatttc aacctcttgg aggagcaagg aactattggt atcagcagcg
     4501 gctgtcctga ggaatcgttt atgtcccata aggcgtattt cactggcaat ggttacctga
     4561 gctcttcaga taaaatctct ccattccaat catttgaagg aggactgagc ttcaaaacac
     4621 tacaacccag cgggctcctg ttctaccaca gagatgggtc ggatgagttt tctctttctc
     4681 tggagaacgg tgctgtggta ctccaggcca aaggaactag agtgaaatcc cagaagaagc
     4741 attacaatga tggccgctct catttcttag tcgcctctgt caccaatcag aaatatgaaa
     4801 ttgtcattga tgacaaagac aagcaggata agaagaagcc gtcttccacg tctcagtcag
     4861 acaacactcc aaaggccttc tactatggag gctctacatc cagttccata ctcaatttca
     4921 ctgggtgcat cagttatgcc tacatcagca ggcaggacag agatatagag ccagaagatt
     4981 tccagcgcta cagtgagaat gtgcaggctt cgctacagga ctgcccgatt gaaagacccc
     5041 cgtctgctct gctcaaagac agcagagact cctctagaag aagtgcaggg cactcccgca
     5101 aggtgggcag ggatgagtcc ggcctgccac atggcctgaa gagcaatcct ctgaacattc
     5161 cagagaaaga ggaggcgtcc tgttacctgt cgtcccgccc acgagcaacc ccccatgcaa
     5221 atcactatgg tggatctgcc cacagcagac atgagtacac gggcatcgcc agtgccatca
     5281 aacacgaatc tcacttctct ctgtctctga aaacccgttc agcgtttggt ctgatcttct
     5341 acctcgccga cacgtctggg gagaacttaa tggctctgtt ccttactcat gcaagactgg
     5401 tattcacgtt cagctcgggc caaagccaag tcagaatccg atccaaggag aagtacaatg
     5461 atggacagtg gcatgatatt atctttatca gagctggaaa tatgggcaga atgctaatcg
     5521 atggtcttac ggtattagag gacagagctc caggcagaaa cacctcgctg cttgtccaag
     5581 accctctcta tgttggcgga gtcccacctc atcaggccat gaaaaacatt cagaggacat
     5641 ctgtgtcgag tttcactggc tgtgtgcgga gtctccagct gaatggccgc tcgctcacct
     5701 ctgcctccca cagttttggg gtcaccccct gctacgaggg cccctcggaa ccggggacgt
     5761 acttcgctga ggagggagga tatgtgctgc tggatgactc ctttcatttc ggctcaagct
     5821 ttaagatggc gatggaggtt cgcccccgtg tggcttcagg ggtgctactg catgtcttca
     5881 tggagcagaa ggaatatctc accgtgtaca tctaccagag ccaggtggtg gttgtggtga
     5941 ataatggtat cgcggagttc tccacccacg tctctcccac acaagaaatc tgtgatggaa
     6001 actggcacaa gatcacagtg atcagagatg gtagcgtgct tcagctggat gtggactcag
     6061 aggtgaatca cgttgtgggg tctgtcagtg acgcagccca gagcagcagt tcgccagtgt
     6121 ttatcggtgg agctccagac tccatgctgt cccacagtct gctcagcagg agaggcttca
     6181 cgggctgcat gaggagcatg tctgtaaacg agacccctgt gagcttcagc aaagctgcgc
     6241 tggtcagtgg atcagtcggg gtgggcgcct gtccttctgc tcacccagct ttcttgtaca
     6301 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca
     6361 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca
     6421 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     6481 cacaagataa aataatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     6541 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
     6601 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     6661 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     6721 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     6781 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     6841 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     6901 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     6961 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     7021 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     7081 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     7141 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     7201 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     7261 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     7321 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     7381 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat
     7441 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7501 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7561 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7621 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     7681 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7741 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7801 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7861 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7921 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7981 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     8041 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     8101 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
