pME-h2afv-EosFPtd vector (V004792)

LOCUS       V004792                 4410 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004792
VERSION     V004792
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Hall TE, Currie PD.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2011) Australian Regenerative Medicine Institute,
            Monash University, Building 75, Melbourne, VIC 3800, Australia
REFERENCE   2  (bases 1 to 4410)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4410)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (27-SEP-2011) Australian Regenerative Medicine Institute, Monash
            University, Building 75, Melbourne, VIC 3800, Australia"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    569..667
                     /label="attL1"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             674..1057
                     /gene="H2AZ2"
                     /label="Histone H2A.V"
                     /note="Histone H2A.V from Bos taurus. Accession#: Q32LA7"
     CDS             1067..1090
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             1130..1804
                     /label="EosFP"
                     /note="green-to-red photoswitchable fluorescent protein
                     (Wiedenmann et al., 2004)"
     CDS             1847..2524
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein
                     (Wiedenmann et al., 2004)"
                     /label="EosFP"
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     protein_bind    complement(2535..2634)
                     /label="attL2"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2652..2670)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2675..2691)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2804..3610
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3760..4348
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"