LOCUS       V004792                 4410 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004792
VERSION     V004792
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Hall TE, Currie PD.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2011) Australian Regenerative Medicine Institute,
            Monash University, Building 75, Melbourne, VIC 3800, Australia
REFERENCE   2  (bases 1 to 4410)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4410)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (27-SEP-2011) Australian Regenerative Medicine Institute, Monash
            University, Building 75, Melbourne, VIC 3800, Australia"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(268..295)
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(387..473)
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     537..553
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    569..667
                     /label="attL1"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             674..1057
                     /gene="H2AZ2"
                     /label="Histone H2A.V"
                     /note="Histone H2A.V from Bos taurus. Accession#: Q32LA7"
     CDS             1067..1090
                     /label="FLAG"
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             1130..1804
                     /label="EosFP"
                     /note="green-to-red photoswitchable fluorescent protein
                     (Wiedenmann et al., 2004)"
     CDS             1847..2524
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein
                     (Wiedenmann et al., 2004)"
                     /label="EosFP"
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     protein_bind    complement(2535..2634)
                     /label="attL2"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2652..2670)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2675..2691)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2804..3610
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3760..4348
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcgcc accatggcag gtggaaaagc aggtaaagac agtggcaaag ccaaggcgaa
      721 agcagtgtct cgctcgcaaa gagctggtct tcagtttcca gtgggacgaa tccacaggca
      781 cttgaagaca cgcactacaa gccatggtcg agtaggagca acagcagccg tttacagcgc
      841 agccattctt gaatatctca cagctgaagt tttggagttg gcgggaaatg cttcaaaaga
      901 cctgaaggtg aagcgcatca ctcctcgaca tttacagctg gccattcgag gagatgagga
      961 gctcgattcc cttatcaagg ccactattgc tggaggaggt gtgattccac acatccacaa
     1021 gtctctgatt ggtaagaagg gccagcagaa aaccgcaggg ggcatggact acaaagacga
     1081 tgacgataaa gaattctgca gatatccatc acactggcgg ccgctcgaga gtgcgattaa
     1141 gccagacatg aagatcaacc tccgtatgga aggcaacgta aacgggcacc actttgtgat
     1201 cgacggagat ggtacaggca agccttttga gggaaaacag agtatggatc ttgaagtcaa
     1261 agagggcgga cctctgcctt ttgcctttga tatcctgacc actgcattcc attacggcaa
     1321 cagggtattc gccgaatatc cagaccacat acaagactat tttaagcagt cgtttcctaa
     1381 ggggtattcg tgggaacgaa gcttgacttt cgaagacggg ggcatttgca ttgccagaaa
     1441 cgacataaca atggaagggg acactttcta taataaagtt cgatttcacg gtgtaaactt
     1501 tcccgccaat ggtccagtta tgcagaagaa gacgctgaaa tgggagccct ccactgagaa
     1561 aatgtatgtg cgtgatggag tgctgaccgg tgatattcgc atggctttgt tgcttgaagg
     1621 aaatgcccat taccgatgtg acttcagaac tacttacaaa gctaaggaga agggtgtcaa
     1681 gttaccaggc taccactttg tggaccactg cattgagatt ttaagccatg acaaagatta
     1741 caacaaggtt aagctgtatg agcatgctgt tgctcattct ggattgcctg acaatgccag
     1801 acgatctaga ggtcacggta ctggttctac tggttctggt tcttctagtg cgattaagcc
     1861 agacatgaag atcaacctcc gtatggaagg caacgtaaac gggcaccact ttgtgatcga
     1921 cggagatggt acaggcaagc cttttgaggg aaaacagagt atggatcttg aagtcaaaga
     1981 gggcggacct ctgccttttg cctttgatat cctgaccact gcattccatt acggcaacag
     2041 ggtattcgcc gaatatccag accacataca agactatttt aagcagtcgt ttcctaaggg
     2101 gtattcgtgg gaacgaagct tgactttcga agacgggggc atttgcattg ccagaaacga
     2161 cataacaatg gaaggggaca ctttctataa taaagttcga tttcacggtg taaactttcc
     2221 cgccaatggt ccagttatgc agaagaagac gctgaaatgg gagccctcca ctgagaaaat
     2281 gtatgtgcgt gatggagtgc tgaccggtga tattcgcatg gctttgttgc ttgaaggaaa
     2341 tgcccattac cgatgtgact tcagaactac ttacaaagct aaggagaagg gtgtcaagtt
     2401 accaggctac cactttgtgg accactgcat tgagatttta agccatgaca aagattacaa
     2461 caaggttaag ctgtatgagc atgctgttgc tcattctgga ttgcctgaca atgccagacg
     2521 ataagggccc tattacccag ctttcttgta caaagttggc attataagaa agcattgctt
     2581 atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc
     2641 agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg
     2701 gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaataatat catcatgaac
     2761 aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg
     2821 ggaaacgtcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
     2881 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga
     2941 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
     3001 gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
     3061 ccgtactcct gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca
     3121 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
     3181 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg
     3241 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga
     3301 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt
     3361 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     3421 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     3481 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     3541 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga
     3601 tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac
     3661 ttgacgggac ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca
     3721 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     3781 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     3841 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     3901 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     3961 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     4021 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     4081 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     4141 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     4201 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     4261 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     4321 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     4381 ggccttttgc tggccttttg ctcacatgtt
//
