pMDS20 vector (V004796)

LOCUS       V004796                 9983 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004796
VERSION     V004796
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9983)
  AUTHORS   Holmes M, Pfeifer F, Dyall-Smith M.
  TITLE     Improved shuttle vectors for Haloferax volcanii including a
            dual-resistance plasmid
  JOURNAL   Gene 146 (1), 117-121 (1994)
   PUBMED   8063095
REFERENCE   2  (bases 1 to 9983)
  AUTHORS   Dyall-Smith ML.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-1994) Microbiology, University of Melbourne,
            Grattan Street, Parkville, Victoria, Australia
REFERENCE   3  (bases 1 to 9983)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9983)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene";
            date: "1994"; volume: "146"; issue: "1"; pages: "117-121"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-MAR-1994) Microbiology, University of Melbourne, Grattan Street,
            Parkville, Victoria, Australia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          894..5001
                     /mol_type="genomic DNA"
                     /note="Haloferax Aa2.2 replicon region; includes origin of
                     replication"
                     /db_xref="taxon:56961"
                     /organism="Shuttle vector pMDS20"
     rep_origin      complement(237..692)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     837..853
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        860..878
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5333..7249
                     /gene="gyrB"
                     /label="DNA gyrase subunit B"
                     /note="DNA gyrase subunit B from Haloferax lucentense
                     (strain DSM 14919 / JCM 9276 / NCIMB 13854 / Aa 2.2).
                     Accession#: P21558"
     promoter        complement(7723..7741)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7762..7778)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    7786..7802
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7810..7840)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7855..7876)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8164..8752)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8926..9783)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9784..9888)
                     /label="AmpR promoter"