LOCUS       V004796                 9983 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004796
VERSION     V004796
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9983)
  AUTHORS   Holmes M, Pfeifer F, Dyall-Smith M.
  TITLE     Improved shuttle vectors for Haloferax volcanii including a
            dual-resistance plasmid
  JOURNAL   Gene 146 (1), 117-121 (1994)
   PUBMED   8063095
REFERENCE   2  (bases 1 to 9983)
  AUTHORS   Dyall-Smith ML.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAR-1994) Microbiology, University of Melbourne,
            Grattan Street, Parkville, Victoria, Australia
REFERENCE   3  (bases 1 to 9983)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9983)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene";
            date: "1994"; volume: "146"; issue: "1"; pages: "117-121"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-MAR-1994) Microbiology, University of Melbourne, Grattan Street,
            Parkville, Victoria, Australia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9983
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          894..5001
                     /mol_type="genomic DNA"
                     /note="Haloferax Aa2.2 replicon region; includes origin of
                     replication"
                     /db_xref="taxon:56961"
                     /organism="Shuttle vector pMDS20"
     rep_origin      complement(237..692)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     837..853
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        860..878
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5333..7249
                     /gene="gyrB"
                     /label="DNA gyrase subunit B"
                     /note="DNA gyrase subunit B from Haloferax lucentense
                     (strain DSM 14919 / JCM 9276 / NCIMB 13854 / Aa 2.2).
                     Accession#: P21558"
     promoter        complement(7723..7741)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7762..7778)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    7786..7802
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7810..7840)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7855..7876)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8164..8752)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8926..9783)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9784..9888)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggaaat
      241 tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
      301 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
      361 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
      421 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
      481 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
      541 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
      601 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
      661 cgccgcgctt aatgcgccgc tacagggcgc gtcgcgccat tcgccattca ggctgcgcaa
      721 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
      781 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
      841 aacgacggcc agtgaattgt aatacgactc actatagggc gaattcgagc tcggtaccgt
      901 attcgaacat cgccgtgttc gcgctaatca cgtcggacga gttgatgttt ccagccgcga
      961 agtcgtcgta cgtcgcattc acgaagttcg acgcctccgc ttggaggtcg tcgttcttcg
     1021 tctcgtaacg cgcccagcgg tcagcgaacg actggtgttc gatgaaagtc atcgaatcgt
     1081 agttcgagtt cggcgcggcg atttcccagc cgcggaagcc cagccgaagt ttccgtctat
     1141 ctgcaaccac tcgtgccggg ccggagtcac gtagtacgtc tcgtaggagc tgacgctgtt
     1201 gtaaccgtct ccgatagtgt agttgtaggc gagcacgtca gcagacgaat cgttgacgag
     1261 agtcacagag gacgtgttca ggccctcgta ggtcttcgag accgaccagc cactcccgct
     1321 atcgacttgt gcgaagtcgt ctttcgtgac gaatgtcgag ttgttaatcg gctccgactc
     1381 ggagcgttct tcgatactca cctcagcggc agagacggtc gcattccaac gggcaatgtc
     1441 gttcttctgc ttgacagcgt agtagtcagc aatcgcccgt ctcgcgtcga tacgggcctg
     1501 ctctttcgtc ttgccgtcct cgtacgcttc agcgacagct acctgcgctt tcatccacgc
     1561 gaccgactcg gtgtcgttca ggtagttgtc catcgccgta ttgtacgagt cgagcgacgt
     1621 cttttcagcc gacgccgccg agtagagctc taccttgtcc tgcgtgccgt cgaggtcgag
     1681 cgtcgagtcg gtcgggttaa accggcactc gtcgccgttc gggttaatca gactgttgat
     1741 gaacgccgaa atcgcggaat ccgagatgtc acagtcttgg aatccgaggg tcgcggaagc
     1801 cggaccggaa gcccctgccg tcacgagcag gacagcaaac aggacagcga gcctgcgtcg
     1861 agcgaagccg cgcatcagag acctccggtg atgatgaacg cagctgcacc gagacacgcg
     1921 gcgaggtagc cgagcgtcgc agtcgcttcc agtctggtga ggccaaaagg aagggtcatg
     1981 gtctggttac ctcacggcga ccgcccagcc gacgaccgta atcaggaacg cgacttgcgc
     2041 gcccagcggg tcgcccagtc cgagcaggag gtccttgatt tcggtcacgt actgccagcc
     2101 gaggatgaca gcaggcgacg cgacgatgaa cgccatctcc cagtactcgt agtgttcgag
     2161 cgatttcgtc tcgctcgaca tgaacgccag cgcgtacacg agaatgccgc cgagcgtcgc
     2221 gtgctcgctc gtcagcgtgt aggacgacag ccacgtgatg gtgatgtcgg agatgccgcc
     2281 gaaggcgtac aggccagtag cgacgaacac gaccgacaag agcgccggga tggtccgcag
     2341 catcgcgtag tccgacgcga ggccaccgag gccctgcgag tagcgcgacg ccattactcg
     2401 cccctcacgt cgaagacgtg gtactcacgc tcctcggtcg agccgtcgtc atgctcgatt
     2461 tcgtacgtcc gggtctcggc ggtcttcttg accgcgagcc aactgccgtt gccgagaccc
     2521 ttcttgtcga tagtccggcg aatctggccg ttcgaccaga acttcactcg gtcaccgagg
     2581 tcgccgccgc gggcgaggtg aatcacgtcg ttttcgttcg gtcccacgtc gcggtcgatg
     2641 tcgacgataa cgccgatgac cggctcgtcc acgtcgaact tgatttcctc tactccgtcg
     2701 tcgtagtcgt cctcgacttg cgtttcttcg aagtcgtcga acgacattgg ttcggatgcc
     2761 attacaacta gatattgtca atactaatct taaagttaag attaggttta gtgaaagtag
     2821 tccgaaacac attcactcaa cctctctcgc cgggtgtcgc atgcacttgc ggtctttgtc
     2881 gaaatagtcc tcaacgtgca gtacgtggtc ggcactctcc ggtcggtcga ggccgtgctg
     2941 ttcgactaag tgacgagcca tccactcagc ttggtagata ccgacgttac actcggaaca
     3001 gcgccacatc tcacctttct gtaacttata ctgaagtcgc gtttcttgca gcacccgctg
     3061 gacggggagt ttcagcggag ccatatcgac gccgccgcct ccgggcaggt gctcctcgcc
     3121 gtcgcggtcg ataatcgctt ccagtcgcca ctcagatgcg agcgatgcgc ggcggaacga
     3181 ttctggttcg gagtcgtcct caccgttcat cactgattcg acgaactcgg catgcttcgg
     3241 gtcaatcatg ttccggccga gcatcgacgc caccgaaggc gtctcgtcgc tgtacttcaa
     3301 ctccgtttcc acgcgcttcc gtatccgcgt ctttgtcggt gattcaccga cactcgccgc
     3361 tgctttggcg ctcggccagc gttcagccag tgagagttcc gaatccgagg cgtccaactc
     3421 gccaccatcc gcgagggctt cggcaaggtc gtcatcagat accggttcgt cgagccggtc
     3481 ctggtcgata gcccagccgg agttacagca cgcgcacact acgtcggggc cgcggccgtc
     3541 gttccagacg actgcttcgc cgtgtgcgtc ggtctggttc gactcgctgg actccgcgcg
     3601 ctgttcgcac gcgtccgctt tgatggcttc cgtgacgatt ttcgaccgcg aggttcggcg
     3661 tcgagcagcc gaccagtaga ttgcgcccca cgcgaggtat tcgaccggct tgtccagcag
     3721 ctcctctgtg tagccgccca tgtacgccgc gaggtacgaa cccatgttct cgacgccggc
     3781 gttcagcgag atgcacccat ccgagtcgtt cagatagtcc gtgtttctgt aatcgtgggc
     3841 gctaaacgag gcgtattcgc actcctcgac gtgtttgtct atcactcgtt cgaactctgg
     3901 gccgaccact tcgaggtcga ggtcggccgc gtcgaagtag acgcccacgt gcaggtgcga
     3961 gtagcacgcg ttcatcccgc tgccgtcgcc acccattccg tgcggttcgg cttggagcca
     4021 gtagccccac tcgtcggcgt cgaggccaga tggtactcca tcgtgttccg cagagtgtcg
     4081 cgcacgccgt cgtacgaaaa cgcgtcgtgg agcgcgtcgg tgtgttcgac cggcggccag
     4141 tcgctccccg ttcggaaccg acgaggcagt gaacgtgagc atcgcggtcg cgggcgagcc
     4201 ccacgccgcg agtgcttctc cgccggacgg tcgttcgccg ccgcccatct gtcgctggac
     4261 gccgagcgcg cgagcgtact gcttgcgcga gtacgtctcg ccccacgcgt cggtcagcgg
     4321 aagttcgaac tcgtcgccgt cgtcgttcgc aaagcgcgcg agcagtcctt tgtagtcgac
     4381 gtgtgaggtg agaaacgcgc tcaacgcgtc cgcccacgtc gctgcgcggc gtttctctaa
     4441 ctccgtcacg tagaacccat tttccatcgg actctctgct tcaacgtacg tatcgcgcca
     4501 gtccgcttcg gtcacgatgc gccggaggtt tcgaccgtgc gtttcgcaca ccggcgtttt
     4561 cggccagttc ggggtatttc tcggtgaact ccagtacgcg ttcgtggact cgttcgtcgt
     4621 agttgtcctc tgctggagaa ccggcggtag acggctttac agagttagtc tccttggaag
     4681 gccgagaggc cacaccgtcg gctggtgagt cgcgcccgcc ggatgcgggg gtgccctcgc
     4741 tactcaccgc tacccccgtc tgtgcggatg aaatcgctca gacggcactg ggccttaccc
     4801 ccgctcgttt tcggccggcg gccgccggcc tcatgcggac cggcttccgg gtcgacccgc
     4861 gaggggtgcc ttccggcagc tcctccctcc cgcgcctcgg ggcactcggc gcggcaggac
     4921 ggtcgtcgcc tctccggtcc ttgcggtggt ttcctcggcc agctggtgcg cccacaggag
     4981 cgaggtgaga cggtggtccc gggggatctc ccgctggccc ttcgccagct cctctgcgat
     5041 ccccggctcg tcaccgagtg tcgactggaa cgaggtcatt tcctgatgcg catctaccca
     5101 gtcccgcgtt aagtactcac cggcagcgga gtgaaagtga actcggggcg ttgacgcgct
     5161 tcgaggggcg ttcgcgccgg aacgccgaat catgatacac agagtcaccc acgaaacgcg
     5221 ggtcggtcgg tcggcgtctc cgatgtcgga aaccgctacg cgggactcca gtaagtagta
     5281 cgcgcccgcg cgtgcgagcc tttaagggac gggctatcct aggcctgttc agatgtctca
     5341 ggataacgag tacggggccg gacagattca ggtcctcgaa gggcttgaag ccgttcgaaa
     5401 gcgtccggcg atgtacatcg ggtccaccga ttctcgcgga ctccaccacc tcgtctacga
     5461 agtcgtcgac aactccatcg acgaggcgct cgcgggccac tgcgacgcca tcgaggtcgc
     5521 cctccacgag gacggctctg tcagcgtcac cgacaacggc cgcgggattc cggtaggtac
     5581 gcacgaacag tacgaccgac ccgcgctgga ggtcatcatg accgtcctcc acgccggcgg
     5641 gaagttcgac aacaagtcct accaagtctc cggcggcctc cacggcgtcg gcgtcaccgt
     5701 cgtcaacgcg ctgtcgagcg aactcgaagt cgaggtcaaa cacgacggcg cggtgtggac
     5761 acaccgcttc gaggtcggcg agccgcaggt cgaggagttc gagcgcgtgc gcgacctcga
     5821 acccggcgaa gacaccggaa cgaccatccg cttttggccg gacgacggta tcttcgagac
     5881 gaccgagttc gacttcaaga cgctcgaaaa ccgcctgcga gagctcgcgt tcctcaactc
     5941 cggggtcgag atttcgctct ccgacgagcg gaccgacgag tcgagcacgt tcctcttcga
     6001 gggcggcatc cgcgagttcg tcgagtacct caacgagacc aagacggcgc tccacgacga
     6061 cgtcatctac tacgacgacg agtcggaggg catcgaggtc gaaatcgcca tgcaggcgac
     6121 cgacgagctc caggggtcga ttcacgcctt cgccaacaac atcaacaccc gcgagggcgg
     6181 cacgcatctg accgggttca agacggcgct cacccgcgtc gtcaacgact acgcgaacag
     6241 ccacgacatg ctggacgacc tcgacggcga caacctccgc ggcgaggacg ttcgcgaggg
     6301 actcaccgcc gtcatctccg tcaagcaccc cgacccgcag ttcgagggcc agacgaagac
     6361 caagctcggg aattccgagg tccgcggcat cgtcgagagc gtcacccacc aacagctcgg
     6421 gacgttcttc gaggagaacc cggacacggc gacggccatc atctcgaagg ccgtcgaggc
     6481 cgcccgcgcc cgcaaggccg cgaagcaggc cgaggagctc acccgccgca agtccgcgct
     6541 cgaatctacc tcgctgccgg ggaagctcgc ggactgccag agccgcgacc cctcggagtc
     6601 cgaactgttc atcgtggagg gcgactccgc gggcgggtcg gccaagcagg gccgcgaccg
     6661 caagttccag gcgattttgc ccctcaaggg gaagattctg aacgtcgaga aacaccgcct
     6721 cgaccgcatt ctcgaaaacg acgagatacg ggcgctcatc accgccattg gcggcggcgt
     6781 cggcgacgag ttcgacatcg agaaggcgcg ctaccagcgg ctcatcctga tgaccgacgc
     6841 cgacgtcgac ggcgcgcaca tccggacgct gctgcttacg cttctgtacc ggcacatgcg
     6901 cccgctcatc gaggccggct acgtgtacgc ggctcaaccg ccgctgtacc gcgtccgcta
     6961 ccgcggcaac acctacgacg cgatggacga ggccgagcgg gaccgcatca tcgaggagga
     7021 atgcaacggc aaccccacgc aggtccagcg gttcaaggga ctcggtgaga tgaaccccga
     7081 ccagctgtgg gacacgacga tgaaccccga aaaccgcgtc ctcaagcgca tcaccgtcga
     7141 ggacgccgcc gccgccgacc ggatgttcaa catcctgatg ggcgacgccg tcgggccgcg
     7201 aaagcagttc atcaaggacc acgcgaacga cgccgaatgg gtagacatct aatatgagtt
     7261 ctgacgcacc agattcgttc gaaccgggcg cgggtatcgc ggccgaagtc aagaacgcgc
     7321 gcatcgaaga cgagatggag cagtcgtaca tcgactacgc gatgtcggtc atcgcggggc
     7381 gggcgctgcc cgacgtacgc gacggcctga agccggtcca ccggcgcatc ctgtatgcga
     7441 tgcataccga cgcgaaagcc gacgcggacg acgagtaacg gctccccgcg agactttttt
     7501 cgcggactcc cgccggagag ccgcggctcc gaccgtcgct ccccgaagac atttatctcg
     7561 gccggaggtc ctaccggtat gagcgtgcga ccgcggtcga gaatcgtcgg cgtctcggcg
     7621 ctcgcgttcg tcgtcgtcct cggcggcctg accgcgcttc gggcgtggaa cctcgaaccg
     7681 ggatcctcta gagtcgacct gcaggcatgc aagcttttgt tccctttagt gagggttaat
     7741 tccgagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     7801 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     7861 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     7921 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     7981 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8041 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8101 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8161 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8221 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8281 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8341 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8401 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8461 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8521 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8581 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     8641 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8701 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     8761 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     8821 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     8881 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     8941 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9001 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9061 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9121 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9181 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9241 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9301 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9361 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9421 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9481 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9541 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9601 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9661 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9721 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     9781 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     9841 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     9901 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
     9961 gcgtatcacg aggccctttc gtc
//
