Basic Vector Information
- Vector Name:
- pMD.SV
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4229 bp
- Type:
- Plasmid vector
- Replication origin:
- R6K γ ori
- Source/Author:
- Dharmasena MN, Stark CEC., Stibitz S, Kopecko DJ.
pMD.SV vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMD.SV vector Sequence
LOCUS 40924_30416 4229 bp DNA circular SYN 18-DEC-2018
DEFINITION Plasmid vector pMD.SV, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4229)
AUTHORS Dharmasena MN, Stark CEC., Stibitz S, Kopecko DJ.
TITLE Acid-resistant, attenuated microbial vector for improved oral
delivery of multiple targeted antigens
JOURNAL Patent: US WO/2015/021147-B 12-FEB-2015; FDA-Center for Biologics
Evaluation and Research; NIH campus Bldg. 29, 8800 Rockville Pike;
Laboratory of Enteric and Sexually Transmitted Diseases; Bethesda,
MD; USA;
REFERENCE 2 (bases 1 to 4229)
AUTHORS Dharmasena MN, Stark CEC., Stibitz S, Kopecko DJ.
TITLE Direct Submission
JOURNAL Submitted (16-MAY-2014) Laboratory of Enteric and Sexually
Transmitted Diseases, FDA-Center for Biologics Evaluation and
Research, NIH Campus Bldg. 29, 8800 Rockville Pike, Bethesda, MD
20892, USA
REFERENCE 3 (bases 1 to 4229)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4229)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Patent: US
WO/2015/021147-B 12-FEB-2015; FDA-Center for Biologics Evaluation
and Research; NIH campus Bldg. 29, 8800 Rockville Pike; Laboratory
of Enteric and Sexually Transmitted Diseases; Bethesda, MD; USA;"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(16-MAY-2014) Laboratory of Enteric and Sexually Transmitted
Diseases, FDA-Center for Biologics Evaluation and Research, NIH
Campus Bldg. 29, 8800 Rockville Pike, Bethesda, MD 20892, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..4229
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 411..1202
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
protein_bind complement(1396..1429)
/label=FRT (minimal)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
CDS 1457..2521
/gene="vexA"
/label=vexA
/note="Vi polysaccharide export protein VexA/TviF from
Salmonella typhi. Accession#: Q04976"
protein_bind complement(2562..2609)
/label=FRT
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
CDS complement(2803..3171)
/label=traJ
/note="oriT-recognizing protein"
oriT complement(3204..3313)
/direction=LEFT
/label=oriT
/note="incP origin of transfer"
rep_origin 3473..3861
/label=R6K gamma ori
/note="gamma replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication"
terminator 3997..4083
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 4175..4202
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
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