LOCUS       V004800                 4229 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004800
VERSION     V004800
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Dharmasena MN, Stark CEC., Stibitz S, Kopecko DJ.
  TITLE     Acid-resistant, attenuated microbial vector for improved oral
            delivery of multiple targeted antigens
  JOURNAL   Patent: US WO/2015/021147-B 12-FEB-2015; FDA-Center for Biologics
            Evaluation and Research; NIH campus Bldg. 29, 8800 Rockville Pike;
            Laboratory of Enteric and Sexually Transmitted Diseases; Bethesda,
            MD; USA;
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Dharmasena MN, Stark CEC., Stibitz S, Kopecko DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2014) Laboratory of Enteric and Sexually
            Transmitted Diseases, FDA-Center for Biologics Evaluation and
            Research, NIH Campus Bldg. 29, 8800 Rockville Pike, Bethesda, MD
            20892, USA
REFERENCE   3  (bases 1 to 4229)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4229)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Patent: US
            WO/2015/021147-B 12-FEB-2015; FDA-Center for Biologics Evaluation
            and Research; NIH campus Bldg. 29, 8800 Rockville Pike; Laboratory
            of Enteric and Sexually Transmitted Diseases; Bethesda, MD; USA;"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-MAY-2014) Laboratory of Enteric and Sexually Transmitted
            Diseases, FDA-Center for Biologics Evaluation and Research, NIH
            Campus Bldg. 29, 8800 Rockville Pike, Bethesda, MD 20892, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4229
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             411..1202
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     protein_bind    complement(1396..1429)
                     /label="FRT (minimal)"
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     CDS             1457..2521
                     /gene="vexA"
                     /label="Vi polysaccharide export protein VexA/TviF"
                     /note="Vi polysaccharide export protein VexA/TviF from
                     Salmonella typhi. Accession#: Q04976"
     protein_bind    complement(2562..2609)
                     /label="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(2803..3171)
                     /label="traJ"
                     /note="oriT-recognizing protein"
     oriT            complement(3204..3313)
                     /direction=LEFT
                     /label="oriT"
                     /note="incP origin of transfer"
     rep_origin      3473..3861
                     /label="R6K gamma ori"
                     /note="gamma replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     terminator      3997..4083
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      4175..4202
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 agattgcagc attacacgtc ttgagcgatt gtgtaggctg gagctgcttc aagatcccct
       61 cacgctgccg caagcactca gggcgcaagg gctgctaaag gaagcggaac acgtagaaag
      121 ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct atctggacaa
      181 gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca tggcgatagc
      241 tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg gcgccctctg
      301 gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca aggatctgat
      361 ggcgcagggg atcaagatct gatcaagaga caggatgagg atcgtttcgc atgattgaac
      421 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact
      481 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc
      541 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg
      601 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg
      661 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt
      721 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc
      781 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag
      841 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg
      901 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc
      961 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt
     1021 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg
     1081 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt
     1141 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct
     1201 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg
     1261 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     1321 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccag
     1381 cttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggaac
     1441 taaggaggat attcatatga aaaaaatcat catattacta acgacatttt tcctgctttc
     1501 gggatgcact attcccaggg cggtatttaa atccagcctt attaatcagg acgatcctcg
     1561 ttataatctg gtcgaagtca cgccgacatt aaaactaagc gctcccgata ctgtgccgaa
     1621 aactattgtc gatccggttt ttgccgcaaa taactggcac tggacatctt tggctaaagg
     1681 cgatgtgctg catatcacta ttttatcctc gggcggggct ggatatttat ccaataacgc
     1741 gagcggcgac cgtgcggatt ttgaaaatat tcttgtgact gacagtaata ccgttcaggt
     1801 gccttatgcc gggacaatcc cggtttctgg attggatgtg acgcaactgg ctgatgagat
     1861 caaaaagcga ctttcgcgcg ttgtcctgaa tcctcaggtg attgtgacac ttaccgcccg
     1921 caccggagcc atggtgacgg tcgagggcag cgggaaaacg ggtcgatacc ctctcgaaca
     1981 gagtatgaat cgtctgagtc atcttttggc aacggcagtg gcagtagaaa ataccagcac
     2041 agatatgatg gaagttcacg tgacgcgcca gcagcattat ttcactgcgc gactgtcaga
     2101 tatttatcag tatcccggat tggatattgc cttacagccg gatgaccgta tcactctgcg
     2161 tcaagtgacc gagtatgtta atgtgctcgg cgcagcaggt gttcagggga aacatgctct
     2221 ggttcagcgt cattccagcg tcgtggatgc tttggcgctc gcgaaaggat taaatgacaa
     2281 tcttgccgat ccgcaagcga tttttctgta caagcataac gaagcggaac aggcgaaaca
     2341 gcagatgcgt aagctgaata tctatcatgt cgatatgagc caacctaact cggtgttttt
     2401 agcgcaggcc atacgagtgg acaacggcga tgttatctat atctcgaacg cctctttgac
     2461 tgatttcgct aaggtgaaag ccgcattcga tagctttttg acccgcggca ctaattcttt
     2521 ctaaggatcc actctcgagg ggcgcgccgg taccgaattc tgaagttcct atactttcta
     2581 gagaatagga acttcggaat aggaacttcg agctcgtcga cctaattcat gcccccgaac
     2641 tgcaggagtg gggaggcacg atggccgctt tggtcggtct tgccttgctc gtcggtgatg
     2701 tacttcacca gctccgcgaa gtcgctcttc ttgatggagc gcatggggac gtgcttggca
     2761 atcacgcgca ccccccggcc gttttagcgg ctaaaaaagt catggctctg ccctcgggcg
     2821 gaccacgccc atcatgacct tgccaagctc gtcctgcttc tcttcgatct tcgccagcag
     2881 ggcgaggatc gtggcatcac cgaaccgcgc cgtgcgcggg tcgtcggtga gccagagttt
     2941 cagcaggccg cccaggcggc ccaggtcgcc attgatgcgg gccagctcgc ggacgtgctc
     3001 atagtccacg acgcccgtga ttttgtagcc ctggccgacg gccagcaggt aggccgacag
     3061 gctcatgccg gccgccgccg ccttttcctc aatcgctctt cgttcgtctg gaaggcagta
     3121 caccttgata ggtgggctgc ccttcctggt tggcttggtt tcatcagcca tccgcttgcc
     3181 ctcatctgtt acgccggcgg tagccggcca gcctcgcaga gcaggattcc cgttgagcac
     3241 cgccaggtgc gaataaggga cagtgaagaa ggaacacccg ctcgcgggtg ggcctacttc
     3301 acctatcctg cccggctgac gccgttggat acaccaagga aagtctacac gaaccctttg
     3361 gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc gaaaaaatcg ctataatgac
     3421 cccgaagcag ggttatgcag cggaaaacgg ccacgatgcg tccggcgtag aggatctgaa
     3481 gatcagcagt tcaacctgtt gatagtacgt actaagctct catgtttcac gtactaagct
     3541 ctcatgttta acgtactaag ctctcatgtt taacgaacta aaccctcatg gctaacgtac
     3601 taagctctca tggctaacgt actaagctct catgtttcac gtactaagct ctcatgtttg
     3661 aacaataaaa ttaatataaa tcagcaactt aaatagcctc taaggtttta agttttataa
     3721 gaaaaaaaag aatatataag gcttttaaag cttttaaggt ttaacggttg tggacaacaa
     3781 gccagggatg taacgcactg agaagccctt agagcctctc aaagcaattt tgagtgacac
     3841 aggaacactt aacggctgac atggcggccg caaaaattaa aaatgaagtt ttaaatcaat
     3901 ctaaagtata tatgagtaaa cttggtctga cagatcgatg gccccccgat ggtagtgtgg
     3961 ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa ggctcagtcg
     4021 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct gagtaggaca
     4081 aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg gcgggcagga
     4141 cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac ggatggcctt
     4201 tttgcgtggc cagtgccaag cttgcatgc
//
