Cart 2

pMC1-EPS-RtGFP vector (V004815)

Basic Vector Information

Vector Name:
pMC1-EPS-RtGFP
Length:
3751 bp
Type:
Cloning vector
Replication origin:
ori
Source/Author:
Madhaiyan M, Peng N, Ngoh ST, Cheng H, Cai L, Fu L, Reddy C, Hong Y, Ji L.
Promoter:
T5

pMC1-EPS-RtGFP vector Vector Map

pMC1-EPS-RtGFP3751 bp60012001800240030003600T5 promotereps promoterEGFPorilox66SV40 poly(A) signalcmlARBSlac operator

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMC1-EPS-RtGFP vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_29911        3751 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMC1-EPS-RtGFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Madhaiyan M, Peng N, Ngoh ST, Cheng H, Cai L, Fu L, Reddy C, Hong Y,
            Ji L.
  TITLE     Synthetic Vector pMC1-EPS-RtGFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Madhaiyan M, Peng N, Ngoh ST, Cheng H, Cai L, Fu L, Reddy C, Hong Y,
            Ji L.
  TITLE     Direct Submission
  JOURNAL   
REFERENCE   3  (bases 1 to 3751)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3751)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-APR-2013) Biomaterials "
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(6..50)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     regulatory      58..753
                     /label=eps promoter
                     /note="eps promoter"
                     /regulatory_class="promoter"
     CDS             754..1470
                     /label=EGFP
                     /note="enhanced GFP"
     rep_origin      1889..2477
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2800..2833
                     /label=lox66
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    2926..3047
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3058..3696)
                     /gene="cmlA"
                     /label=cmlA
                     /note="Chloramphenicol acetyltransferase 2 from Escherichia
                     coli. Accession#: P22615"
     RBS             3703..3714
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    complement(3733..3749)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."

This page is informational only.