LOCUS       V004815                 3751 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004815
VERSION     V004815
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3751)
  AUTHORS   Madhaiyan M, Peng N, Ngoh ST, Cheng H, Cai L, Fu L, Reddy C, Hong Y,
            Ji L.
  TITLE     Synthetic Vector pMC1-EPS-RtGFP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Madhaiyan M, Peng N, Ngoh ST, Cheng H, Cai L, Fu L, Reddy C, Hong Y,
            Ji L.
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3751)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3751)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (30-APR-2013) Biomaterials "
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(6..50)
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     regulatory      58..753
                     /label="eps promoter"
                     /note="eps promoter"
                     /regulatory_class="promoter"
     CDS             754..1470
                     /label="EGFP"
                     /note="enhanced GFP"
     rep_origin      1889..2477
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2800..2833
                     /label="lox66"
                     /note="Right element (RE) mutant of loxP (Araki et al.,
                     2010). Cre-mediated recombination occurs in the 8-bp core
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    2926..3047
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(3058..3696)
                     /gene="cmlA"
                     /label="Chloramphenicol acetyltransferase 2"
                     /note="Chloramphenicol acetyltransferase 2 from Escherichia
                     coli. Accession#: P22615"
     RBS             3703..3714
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     protein_bind    complement(3733..3749)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gaatctatta taattgttat ccgctcacaa agcaaataaa ttttttatga tttctcgact
       61 agttcgttaa cgtaacacct tatgtgatat gacaattgtc acaatgcgat catggataaa
      121 gctgagataa attgcaatgt cagcctgcga tttatcttat tttaggaggt tatttaaaac
      181 gctttaaatg ttattccctt attgtttctg ggattaacta tcactattta aatatggaaa
      241 ataagaaaag tcgcatgggt aataccatgt cagttttttg tcaatatgcg gtaaatccta
      301 aaaagctaaa attttggtct taatattatt cttaaaaaga tgtttatctc tgaatgtcgc
      361 cttgcctgct attcgcttag ctgcaacaat tcctccagca tcgttcaaat ataccagcgt
      421 ctttattttc agcattgtta atcagtgcga aagccggtac ctgccgtatt ttatctggtt
      481 ggcaaatagc gccaccagaa agaggatgta atggatgact atcaatgact tagcgtcaat
      541 cagtgaattg gtaactgaaa gccaggggcg gtagcgtgcc tgcgggtatg tcaatcagcc
      601 tggattattc tgtaatcgca tgtggttgaa taaacgcttt ttaggactga tgccagttat
      661 atttctcaag aattaactgc attctgaatc tgtgtacagc ttgagcgctt atttaaatcg
      721 cacaggataa ttactctgcc aggaggataa tccatggtct cgaagggcga ggagctcttc
      781 accggcgtcg tcccgatcct cgtcgagctc gacggcgacg tcaacggcca caagttctcg
      841 gtctcgggcg agggcgaggg cgacgccacc tacggcaagc tcaccctcaa gttcatctgc
      901 accaccggca agctcccggt cccgtggccg accctcgtca ccaccctcac ctacggcgtc
      961 cagtgcttct cgcgctaccc ggaccacatg aagcagcacg acttcttcaa gtcggccatg
     1021 ccggagggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     1081 cgcgccgagg tcaagttcga gggcgacacc ctcgtcaacc gcatcgagct caagggcatc
     1141 gacttcaagg aggacggcaa catcctcggc cacaagctcg agtacaacta caactcgcac
     1201 aacgtctaca tcatggccga caagcagaag aacggcatca aggtcaactt caagatccgc
     1261 cacaacatcg aggacggctc ggtccagctc gccgaccact accagcagaa caccccgatc
     1321 ggcgacggcc cggtcctcct cccggacaac cactacctct cgacccagtc ggccctctcg
     1381 aaggacccga acgagaagcg cgaccacatg gtcctcctcg agttcgtcac cgccgccggc
     1441 atcaccctcg gcatggacga gctctacaag tagatatctt tcatggtgat gcgtgcctgc
     1501 cgaacattat ggtggaaaat ggccgtttta gcggctttat tgattgcggc cgtctgggcg
     1561 tggcggatcg ttatcaggat attgcgctgg ccacccgtga tattgcggaa gaactgggcg
     1621 gcgaatgggc ggatcgtttt ctggtgctgt atggcattgc ggcaccggat agccagcgta
     1681 ttgcgtttta tcgtctgctg gatgaatttt tctaataact gtcagaccaa gtttactcat
     1741 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     1801 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     1861 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1921 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1981 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     2041 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2101 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2161 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2221 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2281 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2341 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2401 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2461 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2521 ggccttttgc tcattaggca ccccaggctt tacccgaacg accgagcgca gcgagtcagt
     2581 gagcgaggaa gcggagagcg cccaatacgc aaggaaacag ctatgaccat gttaatgcag
     2641 ctggcacgac aggtttcccg actggaaagc gggcagtgaa aggaaggccc atgaggccag
     2701 ttaattaaga ggtacctaga attctgggat ccaagcggcc gctactcgag ataagctttc
     2761 tagattaatt aaggccatga ttgcaggtgg gcgcgccggt accgttcgta taatgtatgc
     2821 tatacgaagt tatttaccac atttgtagag gttttacttg ctttaaaaaa cctcccacat
     2881 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     2941 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     3001 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttagat taatttattt
     3061 cagtatgtta tcacacatca gctgaagtgt attaataaac cgtgctgcat gaaagccatc
     3121 acatactgca tgatgaacct gtacagaaac aggtaataat acgcggtcac cttcctgctg
     3181 aaactttgcc atcgtaaaaa ccggggcaaa ataatcatca tttccggtga tgttcaggtt
     3241 aaatccgtca aaactcaccc acggtaatga tgatatattc aggtgattct ccggtaaatt
     3301 tccctgcgga aacaatctgg tatcatgctg atattctgcc gttaccgcat tataacctgc
     3361 cataaactca ctgagatccg gaaaataacg gcaggacagt gcagagaatg tttcggtttc
     3421 tttatgaaag acagtaaaga ccgggtctga ctggtcccag taaataagtt cattgtcttt
     3481 cagtgccatc cggaactccg gaaactgatt aacagcccgg gagatcaggt aaatcatcag
     3541 cggataaaac ttataacctg tctccgccag tgcggtacgc aaagcggtaa tatcgagttt
     3601 ggtggtcagg ctgaatccgc atttaatctg ctgacgataa agggcaaagt gttccctgcg
     3661 attccaggta ttcaggtcaa tccgggtaaa attcatgatt aatttctcct ctttaatgaa
     3721 ttctgtgtga aattgttatc cgctcacaat t
//
