Basic Vector Information
- Vector Name:
- pMBL5
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4296 bp
- Type:
- Moss transformation vector
- Replication origin:
- ori
- Host:
- Plants
- Source/Author:
- Knight CD, Cove DJ, Cuming AC, Quatrano RS.
- Promoter:
- CaMV 35S
pMBL5 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMBL5 vector Sequence
LOCUS 40924_29886 4296 bp DNA circular SYN 18-DEC-2018 DEFINITION Moss transformation vector pMBL5, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4296) AUTHORS Knight CD, Cove DJ, Cuming AC, Quatrano RS. TITLE Moss Gene Technology JOURNAL (in) Gilmartin,P.M. and Bowler,C. (Eds.); MOLECULAR PLANT BIOLOGY: 285-301; Oxford University Press, Oxford, NY, USA (2002) REFERENCE 2 (bases 1 to 4296) AUTHORS Machuka J, Knight CD, Cove DJ, Cuming AC. TITLE Direct Submission JOURNAL Submitted (30-SEP-2005) Centre for Plant Sciences, Leeds University, Leeds, West Yorkshire LS2 9JT, UK REFERENCE 3 (bases 1 to 4296) TITLE Direct Submission REFERENCE 4 (bases 1 to 4296) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "(in) Gilmartin,P.M. and Bowler,C. (Eds.)"; volume: " MOLECULAR PLANT BIOLOGY"; pages: " 285-301; Oxford University Press, Oxford, NY, USA (2002" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (30-SEP-2005) Centre for Plant Sciences, Leeds University, Leeds, West Yorkshire LS2 9JT, UK" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4296 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind complement(10..26) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(34..50) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(58..88) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(103..124) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(412..1000) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1174..2031) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(2032..2136) /label=AmpR promoter misc_feature 2181..2288 /label=MCS /note="pBluescript multiple cloning site" promoter 2338..2683 /label=CaMV 35S promoter /note="strong constitutive promoter from cauliflower mosaic virus" CDS 2713..3504 /codon_start=1 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3576..3752 /label=CaMV poly(A) signal /note="cauliflower mosaic virus polyadenylation signal"
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