LOCUS       40924_29886        4296 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Moss transformation vector pMBL5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Knight CD, Cove DJ, Cuming AC, Quatrano RS.
  TITLE     Moss Gene Technology
  JOURNAL   (in) Gilmartin,P.M. and Bowler,C. (Eds.); MOLECULAR PLANT BIOLOGY: 
            285-301; Oxford University Press, Oxford, NY, USA (2002)
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Machuka J, Knight CD, Cove DJ, Cuming AC.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2005) Centre for Plant Sciences, Leeds University,
            Leeds, West Yorkshire LS2 9JT, UK
REFERENCE   3  (bases 1 to 4296)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4296)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "(in) 
            Gilmartin,P.M. and Bowler,C. (Eds.)"; volume: " MOLECULAR PLANT 
            BIOLOGY"; pages: " 285-301; Oxford University Press, Oxford, NY, USA
            (2002"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-SEP-2005) Centre for Plant Sciences, Leeds University, Leeds, 
            West Yorkshire LS2 9JT, UK"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4296
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..26)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(34..50)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(58..88)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(103..124)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(412..1000)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1174..2031)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2032..2136)
                     /label=AmpR promoter
     misc_feature    2181..2288
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        2338..2683
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             2713..3504
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3576..3752
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
ORIGIN
        1 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
       61 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      121 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      181 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
      241 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
      301 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
      361 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      421 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      481 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      541 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      601 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      661 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
      721 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
      781 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
      841 ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
      901 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
      961 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     1021 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     1081 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     1141 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     1201 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     1261 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     1321 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     1381 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     1441 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     1501 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     1561 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     1621 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     1681 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     1741 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     1801 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     1861 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     1921 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     1981 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     2041 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     2101 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     2161 tgccagtgcc aagctaattc gagctccacc gcggtggcgg ccgctctaga actagtggat
     2221 cccccgggct gcaggaattc gatatcaagc ttatcgatac cgtcgacctc gagggggggc
     2281 ccggtacccc tactccaaaa atgtcaaaga tacagtctca gaagaccaaa gggctattga
     2341 gacttttcaa caaagggtaa tttcgggaaa cctcctcgga ttccattgcc cagctatctg
     2401 tcacttcatc gaaaggacag tagaaaagga aggtggctcc tacaaatgcc atcattgcga
     2461 taaaggaaag gctatcattc aagatgcctc tgccgacagt ggtcccaaag atggaccccc
     2521 acccncgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
     2581 ttgatgtgac atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga
     2641 cccttcctct atataaggaa gttcatttca tttggagagg acagcccaag catggagaac
     2701 cgacctgcag gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
     2761 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
     2821 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
     2881 ctgaatgaac tccaagacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
     2941 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
     3001 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
     3061 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
     3121 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
     3181 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
     3241 cggatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
     3301 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
     3361 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
     3421 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
     3481 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcggacgg tacgctgaaa
     3541 tcaccagtct cttctacaaa tctatctctc tctattttct ccataaataa tgtgtgagta
     3601 gtttcccgat aagggaaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa
     3661 gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc
     3721 taaaaccaaa atccagtact aaaatccaga tctcctaaag tccctataga tctttgtcgt
     3781 gaatataaac cagacacgag acgactaaac ctggagccca gacgccgttc gaagctagaa
     3841 gtaccgctta ggcaggaggc cgttagggaa aagatgctaa ggcagggttg gttacgttga
     3901 ctcccccgta ggtttggttt aaatatgatg aagtggacgg aaggaaggag gaagacaagg
     3961 aaggataagg ttgcaggccc tgtgcaaggt aagaagatgg aaatttgata gaggtacgct
     4021 actatactta tactatacgc taagggaatg cttgtattta taccctatac cccctaataa
     4081 ccccttatca atttaagaaa taatccgcat aagcccccgc ttaaaaattg gtatcagagc
     4141 catgaatagg tctatgacca aaactcaaga ggataaaacc tcaccaaaat acgaaagagt
     4201 tcttaactct aaagataaaa gatctttcaa gatcaaaact agttccctca caccggtgac
     4261 ggggatcgca tgcgatatct cgagatctag cttggc
//
