Basic Vector Information
- Vector Name:
- pMB1.MjYRS
- Antibiotic Resistance:
- Streptomycin
- Length:
- 5655 bp
- Type:
- Cloning vector
- Replication origin:
- p15A ori
- Source/Author:
- Tack DS, Ellefson JW, Thyer R, Wang B, Gollihar J, Forster MT, Ellington AD.
pMB1.MjYRS vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMB1.MjYRS vector Sequence
LOCUS 40924_29871 5655 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pMB1.MjYRS, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5655)
AUTHORS Tack DS, Ellefson JW, Thyer R, Wang B, Gollihar J, Forster MT,
Ellington AD.
TITLE Addicting diverse bacteria to a non-canonical amino acid
JOURNAL Nat. Chem. Biol. (2016) In press
REFERENCE 2 (bases 1 to 5655)
AUTHORS Tack DS, Ellefson JW, Thyer R, Wang B, Gollihar J, Forster MT,
Ellington AD.
TITLE Direct Submission
JOURNAL Submitted (06-NOV-2015) Molecular Biosciences, University of Texas,
2500 Speedway, Austin, TX 78660, USA
REFERENCE 3 (bases 1 to 5655)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5655)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem.
Biol. (2016) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(06-NOV-2015) Molecular Biosciences, University of Texas, 2500
Speedway, Austin, TX 78660, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..5655
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 1440..2228
/label=SmR
/note="aminoglycoside adenylyltransferase (Murphy, 1985)"
promoter complement(2342..2444)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
rep_origin complement(2970..3515)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
regulatory 3653..3800
/gene="tyrS"
/regulatory_class="promoter"
CDS 3814..4731
/gene="tyrS"
/label=tyrS
/note="Tyrosine--tRNA ligase from Methanocaldococcus
jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
10045 / NBRC 100440). Accession#: Q57834"
regulatory 4911..4959
/gene="lpp"
/regulatory_class="promoter"
gene 4911..4959
/gene="lpp"
/label=lpp
tRNA 4977..5053
/product="tRNA-Tyr"
/label=3-iodo-tyrosine non-canonical amino acid
/note="3-iodo-tyrosine non-canonical amino acid"
regulatory 5078..5107
/gene="rrnB"
/regulatory_class="terminator"
gene 5078..5107
/gene="rrnB"
/label=rrnB
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