LOCUS       V004822                 5655 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004822
VERSION     V004822
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Tack DS, Ellefson JW, Thyer R, Wang B, Gollihar J, Forster MT,
            Ellington AD.
  TITLE     Addicting diverse bacteria to a non-canonical amino acid
  JOURNAL   Nat. Chem. Biol. (2016) In press
REFERENCE   2  (bases 1 to 5655)
  AUTHORS   Tack DS, Ellefson JW, Thyer R, Wang B, Gollihar J, Forster MT,
            Ellington AD.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2015) Molecular Biosciences, University of Texas,
            2500 Speedway, Austin, TX 78660, USA
REFERENCE   3  (bases 1 to 5655)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5655)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem.
            Biol. (2016) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-NOV-2015) Molecular Biosciences, University of Texas, 2500
            Speedway, Austin, TX 78660, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1440..2228
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     promoter        complement(2342..2444)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(2970..3515)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     regulatory      3653..3800
                     /gene="tyrS"
                     /regulatory_class="promoter"
     CDS             3814..4731
                     /gene="tyrS"
                     /label="Tyrosine--tRNA ligase"
                     /note="Tyrosine--tRNA ligase from Methanocaldococcus
                     jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
                     10045 / NBRC 100440). Accession#: Q57834"
     regulatory      4911..4959
                     /gene="lpp"
                     /regulatory_class="promoter"
     gene            4911..4959
                     /gene="lpp"
                     /label="lpp"
     tRNA            4977..5053
                     /product="tRNA-Tyr"
                     /label="3-iodo-tyrosine non-canonical amino acid"
                     /note="3-iodo-tyrosine non-canonical amino acid"
     regulatory      5078..5107
                     /gene="rrnB"
                     /regulatory_class="terminator"
     gene            5078..5107
                     /gene="rrnB"
                     /label="rrnB"
ORIGIN
        1 attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc
       61 cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa
      121 gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga
      181 acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg ggcagcgttg
      241 ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc
      301 ggggttgcct tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact
      361 gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt
      421 cgtaaagtct ggaaacgcgg aagtccccta cgtgctgctg aagttgcccg caacagagag
      481 tggaaccaac cggtgatacc acgatactat gactgagagt caacgccatg agcggcctca
      541 tttcttattc tgagttacaa cagtccgcac cgctgccggt agctccttcc ggtgggcgcg
      601 gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca
      661 ggtgccggca gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
      721 acaagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct ggctaccctg
      781 tggaacacct acatctgtat taacgaagcg ctaaccgttt ttatcaggct ctgggaggca
      841 gaataaatga tcatatcgtc aattattacc tccacgggga gagcctgagc aaactggcct
      901 caggcatttg agaagcacac ggtcacactg cttccggtag tcaataaacc ggtaaaccag
      961 caatagacat aagcggctat ttaacgaccc tgcccggcag atccgtgcac agcaccttgc
     1021 cgtagaagaa cagcaaggcc gccaatgcct gacgatgcgt ggagaccgaa accttgcgct
     1081 cgttcgccag ccaggacaga aatgcctcga cttcgctgct gcccaaggtt gccgggtgac
     1141 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta
     1201 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca
     1261 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac
     1321 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt
     1381 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatca
     1441 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg
     1501 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg
     1561 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa
     1621 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg
     1681 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt
     1741 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta
     1801 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac
     1861 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg
     1921 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg
     1981 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca
     2041 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc
     2101 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct
     2161 cgcgcgcaga tcagttggaa gaatttgccc actacgtgaa aggcgagatc accaaggtag
     2221 tcggcaaata atgtctaaca attcgttcaa gccgacgccg cttcgcggcg cggcttaact
     2281 caagcgttag atgcactaag cacataattg ctcacagcca aactatcagg tcaagtctgc
     2341 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     2401 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc
     2461 aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga
     2521 agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact
     2581 tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc
     2641 tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat
     2701 cagcgctagc ggagtgtata ctggcttact atgttggcac tgatgagggt gtcagtgaag
     2761 tgcttcatgt ggcaggagaa aaaaggctgc accggtgcgt cagcagaata tgtgatacag
     2821 gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc
     2881 ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag
     2941 ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat
     3001 cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag
     3061 gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
     3121 gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta
     3181 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc
     3241 cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc
     3301 agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct
     3361 aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag
     3421 agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag
     3481 agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt aatcagataa
     3541 aatatttcta gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca
     3601 tacgatataa gttgtaattc tcatgtttga cagcattatc atcgataagc ttgatgcgtg
     3661 gaagattgat cgtcttgcac cctgaaaaga tgcaaaaatc ttgctttaat cgctggtact
     3721 cctgattctg gcactttatt ctatgtctct ttcgcatctg gcgaaaagtc gtgtaccggc
     3781 aaaggtgcag tcgttatata cttggagatt catatggacg agttcgagat gatcaaacgc
     3841 aacaccagcg aaatcatcag cgaagaagaa ctgcgcgaag tgctgaaaaa agacgagaag
     3901 agcgcctata ttggtttcga accgagcggc aaaattcatc tgggccacta cctgcagatc
     3961 aagaagatga tcgatctgca gaacgcgggc ttcgatatca tcattctgct ggccgatctg
     4021 catgcatacc tgaaccagaa aggcgaactg gacgaaatcc gcaaaatcgg cgactacaac
     4081 aagaaagtgt tcgaagcgat gggcctgaaa gcgaaatacg tctatggcag cgaatttcag
     4141 ctggacaaag actacaccct gaacgtgtat cgtctggcac tgaaaaccac cctgaaacgt
     4201 gctcgtcgtt ctatggaact gattgcccgc gaagatgaaa acccgaaagt ggcggaagtg
     4261 atctatccga tcatgcaggt gaacgacatc cactatttgg gcgtagatgt agcagttggt
     4321 ggcatggaac agcgcaaaat ccacatgctg gctcgtgaac tgctgccgaa aaaagtcgtg
     4381 tgcatccaca acccggtttt aacgggtctg gatggtgaag gcaaaatgag cagcagcaaa
     4441 ggcaacttta tcgccgtgga tgattctccg gaagaaatcc gcgcgaagat caaaaaagcg
     4501 tattgcccgg caggtgtagt tgaaggtaac ccgatcatgg agatcgcgaa atacttcctg
     4561 gaatacccgc tgaccattaa acgcccggaa aaattcggtg gtgatctgac cgtgaacagc
     4621 tacgaagaac tggagagcct gttcaagaac aaagaactgc acccgatgga tctgaaaaat
     4681 gccgttgcgg aagagctgat caaaatcctg gaaccgattc gcaaacgtct gtaagatctg
     4741 gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
     4801 cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
     4861 gcatgcggcg ccgcttcttt gagcgaacga tcaaaaataa gtggcgcccc atcaaaaaaa
     4921 tattctcaac ataaaaaact ttgtgtaata cttgtaacgc tgccatcaga cgcattccgg
     4981 cggtagttca gcagggcaga acggcggact ctaaatccgc aggtcgctgg ttcaaatccg
     5041 gcccgccgga ccatttatca cagattggaa atttttgatc cttagcgaaa gctaaggatt
     5101 ttttttagtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg
     5161 cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga
     5221 caggtgccgg cagcgctctg ggtcattttc ggcgaggacc gctttcgctg gagcgcgacg
     5281 atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg ccctcgctca agccttcgtc
     5341 actggtcccg ccaccaaacg tttcggcgag aagcaggcca ttatcgccgg catggcggcc
     5401 gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag gctggatggc cttccccatt
     5461 atgattcttc tcgcttccgg cggcatcggg atgcccgcgt tgcaggccat gctgtccagg
     5521 caggtagatg acgaccatca nggacagctt caaggatcgc tcgcggctct taccagccta
     5581 acttcgatca ttggaccgct gatcgtcacg gcgatttatg ccgcctcggc gagcacatgg
     5641 aacgggttgg catgg
//
