pMB1.3iYRS vector (V004823)

LOCUS       V004823                 5320 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004823
VERSION     V004823
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Tack DS, Ellefson JW, Thyer R, Wang B, Gollihar J, Forster MT,
            Ellington AD.
  TITLE     Addicting diverse bacteria to a non-canonical amino acid
  JOURNAL   Nat. Chem. Biol. (2016) In press
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Tack DS, Ellefson JW, Thyer R, Wang B, Gollihar J, Forster MT,
            Ellington AD.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2015) Molecular Biosciences, University of Texas,
            2500 Speedway, Austin, TX 78660, USA
REFERENCE   3  (bases 1 to 5320)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5320)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem.
            Biol. (2016) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-NOV-2015) Molecular Biosciences, University of Texas, 2500
            Speedway, Austin, TX 78660, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1665..2195)
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     promoter        complement(2527..2629)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(3155..3700)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     regulatory      3838..3985
                     /gene="tyrS"
                     /regulatory_class="promoter"
     CDS             3999..4916
                     /gene="tyrS"
                     /label="Tyrosine--tRNA ligase"
                     /note="Tyrosine--tRNA ligase from Methanocaldococcus
                     jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
                     10045 / NBRC 100440). Accession#: Q57834"
     regulatory      5096..5144
                     /gene="lpp"
                     /regulatory_class="promoter"
     gene            5096..5144
                     /gene="lpp"
                     /label="lpp"
     tRNA            5162..5238
                     /gene="nap3"
                     /product="tRNA-Tyr"
                     /label="nap3"
                     /note="3-iodo-tyrosine non-canonical amino acid"
     gene            5162..5238
                     /gene="nap3"
                     /label="nap3"
     regulatory      5263..5292
                     /gene="rrnB"
                     /regulatory_class="terminator"
     gene            5263..5292
                     /gene="rrnB"
                     /label="rrnB"