Basic Vector Information
- Vector Name:
- pMB1.3iYRS
- Antibiotic Resistance:
- Gentamicin
- Length:
- 5320 bp
- Type:
- Cloning vector
- Replication origin:
- p15A ori
- Source/Author:
- Tack DS, Ellefson JW, Thyer R, Wang B, Gollihar J, Forster MT, Ellington AD.
pMB1.3iYRS vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMB1.3iYRS vector Sequence
LOCUS 40924_29866 5320 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pMB1.3iYRS, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5320)
AUTHORS Tack DS, Ellefson JW, Thyer R, Wang B, Gollihar J, Forster MT,
Ellington AD.
TITLE Addicting diverse bacteria to a non-canonical amino acid
JOURNAL Nat. Chem. Biol. (2016) In press
REFERENCE 2 (bases 1 to 5320)
AUTHORS Tack DS, Ellefson JW, Thyer R, Wang B, Gollihar J, Forster MT,
Ellington AD.
TITLE Direct Submission
JOURNAL Submitted (06-NOV-2015) Molecular Biosciences, University of Texas,
2500 Speedway, Austin, TX 78660, USA
REFERENCE 3 (bases 1 to 5320)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5320)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem.
Biol. (2016) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(06-NOV-2015) Molecular Biosciences, University of Texas, 2500
Speedway, Austin, TX 78660, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Sequencing Technology :: Sanger dideoxy sequencing
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..5320
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(1665..2195)
/label=GmR
/note="gentamycin acetyltransferase"
promoter complement(2527..2629)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
rep_origin complement(3155..3700)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin."
regulatory 3838..3985
/gene="tyrS"
/regulatory_class="promoter"
CDS 3999..4916
/gene="tyrS"
/label=tyrS
/note="Tyrosine--tRNA ligase from Methanocaldococcus
jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
10045 / NBRC 100440). Accession#: Q57834"
regulatory 5096..5144
/gene="lpp"
/regulatory_class="promoter"
gene 5096..5144
/gene="lpp"
/label=lpp
tRNA 5162..5238
/gene="nap3"
/product="tRNA-Tyr"
/label=nap3
/note="3-iodo-tyrosine non-canonical amino acid"
gene 5162..5238
/gene="nap3"
/label=nap3
regulatory 5263..5292
/gene="rrnB"
/regulatory_class="terminator"
gene 5263..5292
/gene="rrnB"
/label=rrnB
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