LOCUS       V004823                 5320 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V004823
VERSION     V004823
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Tack DS, Ellefson JW, Thyer R, Wang B, Gollihar J, Forster MT,
            Ellington AD.
  TITLE     Addicting diverse bacteria to a non-canonical amino acid
  JOURNAL   Nat. Chem. Biol. (2016) In press
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Tack DS, Ellefson JW, Thyer R, Wang B, Gollihar J, Forster MT,
            Ellington AD.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-NOV-2015) Molecular Biosciences, University of Texas,
            2500 Speedway, Austin, TX 78660, USA
REFERENCE   3  (bases 1 to 5320)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5320)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat. Chem.
            Biol. (2016) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (06-NOV-2015) Molecular Biosciences, University of Texas, 2500
            Speedway, Austin, TX 78660, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1665..2195)
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     promoter        complement(2527..2629)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(3155..3700)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
     regulatory      3838..3985
                     /gene="tyrS"
                     /regulatory_class="promoter"
     CDS             3999..4916
                     /gene="tyrS"
                     /label="Tyrosine--tRNA ligase"
                     /note="Tyrosine--tRNA ligase from Methanocaldococcus
                     jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
                     10045 / NBRC 100440). Accession#: Q57834"
     regulatory      5096..5144
                     /gene="lpp"
                     /regulatory_class="promoter"
     gene            5096..5144
                     /gene="lpp"
                     /label="lpp"
     tRNA            5162..5238
                     /gene="nap3"
                     /product="tRNA-Tyr"
                     /label="nap3"
                     /note="3-iodo-tyrosine non-canonical amino acid"
     gene            5162..5238
                     /gene="nap3"
                     /label="nap3"
     regulatory      5263..5292
                     /gene="rrnB"
                     /regulatory_class="terminator"
     gene            5263..5292
                     /gene="rrnB"
                     /label="rrnB"
ORIGIN
        1 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt
       61 cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga
      121 ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt
      181 gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca
      241 ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac
      301 gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc
      361 cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcanggac agcttcaagg
      421 atcgctcgcg gctcttacca gcctaacttc gatcattgga ccgctgatcg tcacggcgat
      481 ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata
      541 ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat
      601 ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc
      661 ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat cgcgtccgcc
      721 atctccagca gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc
      781 atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct tactggttag
      841 cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg
      901 acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg
      961 aagtccccta cgtgctgctg aagttgcccg caacagagag tggaaccaac cggtgatacc
     1021 acgatactat gactgagagt caacgccatg agcggcctca tttcttattc tgagttacaa
     1081 cagtccgcac cgctgccggt agctccttcc ggtgggcgcg gggcatgact atcgtcgccg
     1141 cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgcccaaca
     1201 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgccc tgcaccatta
     1261 tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat
     1321 taacgaagcg ctaaccgttt ttatcaggct ctgggaggca gaataaatga tcatatcgtc
     1381 aattattact tccacgggga gannngagca aactggcctc aggcatttga gaagcacacg
     1441 gtcacactgc ttccggtagt caataaaccg gtaaaccagc aatagacata agcggctatt
     1501 taacgaccct gcccngaacc gacgaccggg tcgaatttgc tttcgaattt ctgccattca
     1561 tccgcttatt atcacttatt caggcgtagc aaccaggcgt ttaagggcac caataactgc
     1621 cttaaaaaaa ttacgccccg ccctgccact catcgcagta cttaggtggc ggtacttggg
     1681 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     1741 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     1801 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     1861 gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc
     1921 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     1981 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     2041 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     2101 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     2161 ccctgctgcg taacatcgtt gctgctgcgt aacataacac cccttgtact actgtttatg
     2221 taagtagaca gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag
     2281 agattttgag acacaacgtg gctttccgaa ttccggatga gcattcatca ggcgggcaag
     2341 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
     2401 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
     2461 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
     2521 ctccatttta gcttccttag ctcctgaaaa tctcgataac tcaaaaaata cgcccggtag
     2581 tgatcttatt tcattatggt gaaagttgga acctcttacg tgccgatcaa cgtctcattt
     2641 tcgccaaaag ttggcccagg gcttcccggt atcaacaggg acaccaggat ttatttattc
     2701 tgcgaagtga tcttccgtca caggtattta ttcggcgcaa agtgcgtcgg gtgatgctgc
     2761 caacttactg atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta
     2821 tcagctgtcc ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcaccgccg
     2881 gacatcagcg ctagcggagt gtatactggc ttactatgtt ggcactgatg agggtgtcag
     2941 tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca gaatatgtga
     3001 tacaggatat attccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg
     3061 cgagcggaaa tggcttacga acggggcgga gatttcctgg aagatgccag gaagatactt
     3121 aacagggaag tgagagggcc gcggcaaagc cgtttttcca taggctccgc ccccctgaca
     3181 agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga ctataaagat
     3241 accaggcgtt tccccctggc ggctccctcg tgcgctctcc tgttcctgcc tttcggttta
     3301 ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac actcagttcc
     3361 gggtaggcag ttcgctccaa gctggactgt atgcacgaac cccccgttca gtccgaccgc
     3421 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg aaagacatgc aaaagcacca
     3481 ctggcagcag ccactggtaa ttgatttaga ggagttagtc ttgaagtcat gcgccggtta
     3541 aggctaaact gaaaggacaa gttttggtga ctgcgctcct ccaagccagt tacctcggtt
     3601 caaagagttg gtagctcaga gaaccttcga aaaaccgccc tgcaaggcgg ttttttcgtt
     3661 ttcagagcaa gagattacgc gcagaccaaa acgatctcaa gaagatcatc ttattaatca
     3721 gataaaatat ttctagattt cagtgcaatt tatctcttca aatgtagcac ctgaagtcag
     3781 ccccatacga tataagttgt aattctcatg tttgacagca ttatcatcga taagcttgat
     3841 gcgtggaaga ttgatcgtct tgcaccctga aaagatgcaa aaatcttgct ttaatcgctg
     3901 gtactcctga ttctggcact ttattctatg tctctttcgc atctggcgaa aagtcgtgta
     3961 ccggcaaagg tgcagtcgtt atatacttgg agattcatat ggacgaattt gaaatgataa
     4021 agagaaacac atctgaaatt atcagcgagg aagagttaag agaggtttta aaaaaagatg
     4081 aaaaatctgc ttacataggt tttgaaccaa gtggtaaaat acatttaggg cattatctcc
     4141 aaataaaaaa gatgattgat ttacaaaatg ctggatttga tataattata ttgttggctg
     4201 atttagccgc ctatttaaac cagaaaggag agttggatga gattagaaaa ataggagatt
     4261 ataacaaaaa agtttttgaa gcaatggggt taaaggcaaa atatgtttat ggaagtgaat
     4321 tccagcttga taaggattat acactgaatg tctatagatt ggctttaaaa actaccttaa
     4381 aaagagcaag aaggagtatg gaacttatag caagagagga tgaaaatcca aaggttgctg
     4441 aagttatcta tccaataatg caggttaata ccagtcatta tttaggcgtt gatgttgcag
     4501 ttggagggat ggagcagaga aaaatacaca tgttagcaag ggagctttta ccaaaaaagg
     4561 ttgtttgtat tcacaaccct gtcttaacgg gtttggatgg agaaggaaag atgagttctt
     4621 caaaagggaa ttttatagct gttgatgact ctccagaaga gattagggct aagataaaga
     4681 aagcatactg cccagctgga gttgttgaag gaaatccaat aatggagata gctaaatact
     4741 tccttgaata tcctttaacc ataaaaaggc cagaaaaatt tggtggagat ttgacagtta
     4801 atagctatga ggagttagag agtttattta aaaataagga attgcatcca atgtatttaa
     4861 aaaatgctgt agctgaagaa cttataaaga ttttagagcc aattagaaag agattataag
     4921 atctggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     4981 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     5041 atgaggcatg cggcgccgct tctttgagcg aacgatcaaa aataagtggc gccccatcaa
     5101 aaaaatattc tcaacataaa aaactttgtg taatacttgt aacgctgcca tcagacgcat
     5161 tccggcggta gttcagcagg gcagaacggc ggactctaaa tccgcaggtc gctggttcaa
     5221 atccggcccg ccggaccatt tatcacagat tggaaatttt tgatccttag cgaaagctaa
     5281 ggattttttt tagtcgaccg atgcccttga gagccttcaa
//
