Basic Vector Information
- Vector Name:
- pMAT3
- Antibiotic Resistance:
- Tetracycline
- Length:
- 7275 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Gomez-Santos N, Treuner-Lange A, Moraleda-Munoz A, Garcia-Bravo E, Garcia-Hernandez R, Martinez-Cayuela M, Perez J, Sogaard-Andersen L, Munoz-Dorado J.
- Promoter:
- tet
pMAT3 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMAT3 vector Sequence
LOCUS 40924_29826 7275 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pMAT3, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7275)
AUTHORS Gomez-Santos N, Treuner-Lange A, Moraleda-Munoz A, Garcia-Bravo E,
Garcia-Hernandez R, Martinez-Cayuela M, Perez J, Sogaard-Andersen L,
Munoz-Dorado J.
TITLE Comprehensive Set of Integrative Plasmid Vectors for
Copper-Inducible Gene Expression in Myxococcus xanthus
JOURNAL Appl. Environ. Microbiol. 78 (8), 2515-2521 (2012)
PUBMED 22287008
REFERENCE 2 (bases 1 to 7275)
AUTHORS Treuner-Lange A.
TITLE Direct Submission
JOURNAL Submitted (26-OCT-2011) Microbiology, University of Granada (Spain),
Avda. Fuentenueva s/n, Granada, Granada 18071, Spain
REFERENCE 3 (bases 1 to 7275)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7275)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Appl.
Environ. Microbiol."; date: "2012"; volume: "78"; issue: "8"; pages:
"2515-2521"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(26-OCT-2011) Microbiology, University of Granada (Spain), Avda.
Fuentenueva s/n, Granada, Granada 18071, Spain"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7275
/mol_type="other DNA"
/organism="synthetic DNA construct"
regulatory 7..830
/label=cuoA promoter
/note="cuoA promoter"
/regulatory_class="promoter"
misc_feature 831..859
/label=multicloning site
/note="multicloning site"
primer_bind complement(863..879)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(1214..2401)
/label=TcR
/note="tetracycline efflux protein"
promoter complement(2449..2477)
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
misc_recomb 2903..5882
/label=att site
/note="att site"
rep_origin 5983..6574
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(6767..6909)
/label=bom
/note="basis of mobility region from pBR322"
protein_bind 7152..7173
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 7188..7218
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7226..7242
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 7250..7266
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
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