LOCUS       40924_29826        7275 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMAT3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7275)
  AUTHORS   Gomez-Santos N, Treuner-Lange A, Moraleda-Munoz A, Garcia-Bravo E, 
            Garcia-Hernandez R, Martinez-Cayuela M, Perez J, Sogaard-Andersen L,
            Munoz-Dorado J.
  TITLE     Comprehensive Set of Integrative Plasmid Vectors for 
            Copper-Inducible Gene Expression in Myxococcus xanthus
  JOURNAL   Appl. Environ. Microbiol. 78 (8), 2515-2521 (2012)
  PUBMED    22287008
REFERENCE   2  (bases 1 to 7275)
  AUTHORS   Treuner-Lange A.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2011) Microbiology, University of Granada (Spain),
            Avda. Fuentenueva s/n, Granada, Granada 18071, Spain
REFERENCE   3  (bases 1 to 7275)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7275)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "2012"; volume: "78"; issue: "8"; pages:
            "2515-2521"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-OCT-2011) Microbiology, University of Granada (Spain), Avda. 
            Fuentenueva s/n, Granada, Granada 18071, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..830
                     /label=cuoA promoter
                     /note="cuoA promoter"
                     /regulatory_class="promoter"
     misc_feature    831..859
                     /label=multicloning site
                     /note="multicloning site"
     primer_bind     complement(863..879)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1214..2401)
                     /label=TcR
                     /note="tetracycline efflux protein"
     promoter        complement(2449..2477)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     misc_recomb     2903..5882
                     /label=att site
                     /note="att site"
     rep_origin      5983..6574
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(6767..6909)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     protein_bind    7152..7173
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7188..7218
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7226..7242
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7250..7266
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcggcc atgaacggca cttcacggct ccgtggaacg acgctgctcc acgttccacc
       61 tgtgaccgtg acggttacag cgaaacccat ccgggtactc cggacaggtg ccgcgccacg
      121 gcaaccccgc atgaacaggc tcgctcagcc ctcggcacgc gttgtgctcc agcggaccga
      181 cttcacggga tgccggacgt cctcgagagc atcctcactc tgggagcaac caagcgatga
      241 agaagagcct gatgggactc cttgctgcac tccccctgat tgccgcggcc cccgcggtcg
      301 cgtgtgacga gcacgccaag cacggcgagc acgccagcca cggcgagcac gccaagcacg
      361 gcgatcattc cgcgctcgga ggcacggcca agaaggccgc tgcgcccgca cagaagcccg
      421 cctcggcccc ggccaccagg aatggcgtcc agaccgtgga cctcacggtg acccccaagg
      481 gcttcgagcc ctcggacgtg aaggtgaagg cggggcagcc cgtccgcctc gtggtcacgc
      541 gcaagacggc caagacgtgc gccacggaaa tcgtcatggc cgacctgggc atcaacaagc
      601 cgctccccat ggacacgccg gtgaccgtgg agttcacccc cagcaagtcg ggcacgctgc
      661 gctacgcctg cgcgatggac cacatcagcg ggctcgtcac catccagtag accgccgttc
      721 cgggcaggct gcgcaccgag gtcacgcgcc tcggggccgg gccacgccac cacggcggcc
      781 tggtcccctc ctcgcccgca gcttccatcc attcgtgaag aggcttcatg tctagactcg
      841 aggatccctg cagaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc
      901 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
      961 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgcc
     1021 cctgtaatcc gggcagcgca acggaacatt catcagtgta aaaatggaat caataaagcc
     1081 ctgcgcagcg cgcagggtca gcctgaatac gcgcaagggt tggtttgcgc attcacagtt
     1141 ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc
     1201 cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc
     1261 agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg
     1321 cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg
     1381 cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct
     1441 gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc
     1501 agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga
     1561 taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga
     1621 gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa
     1681 agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga
     1741 taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc
     1801 tgactgggtt gaaggctctc aagggcatcg gtcggcgctc tcccttatgc gactcctgca
     1861 ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg
     1921 catgtaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc
     1981 cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg
     2041 cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg
     2101 cgtagagaat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa
     2161 gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg
     2221 gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga
     2281 tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta
     2341 tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca
     2401 tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagctaat
     2461 cgatgataag ctgtcaaaca tgagaattac gcgtattcag gctgaccctg cgcgctgcgc
     2521 agggctttat tgattccatt tttacactga tgaatgttcc gttgcgcaaa ctattaactg
     2581 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2641 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2701 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2761 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2821 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttaat
     2881 catatatact ttagattgat ttgggatgcg gtggaccatg agcgggaagc ggtcggggag
     2941 cgtatcgggg ctatcttcgg gcggacgggt agtgctgcgc ggctttcgcg acatggagga
     3001 ctcctctggc tgggtgggtc gacggtgact gcgggtgaga ctcaggtagc ggaagggctc
     3061 tcgccgtccg ccacggcgcg gcaggccggg cagtcggacc atcggtcatc ggcggcatgg
     3121 gtgcggtgca gggcggccgc aagccgctcc agggccatca gggaggcgtc cacgagttgg
     3181 gacgccagca ccttgcgctc acgtccggcg ccggacttga ggacccccat ctgctgacgc
     3241 accacggcaa gcggatgggg cgctgtcacg ggcttgtgcc agtcaactgc gccagtccgt
     3301 gccagtttgg cggactccgc gtctaagccc tcagattttg ttggcaactg gcgcggattg
     3361 gcgtagaagg agcccaggag tacgggttca agtcccgtac tcctcgccac ccctgcccca
     3421 gcaagttctc ggacttgctg gggtttttct tttccagcgt ccgctgtcac accgctgtca
     3481 cagcttggca ctcggcaggc catggtttcc tcctcctgct tgggcagttc gagcttcaac
     3541 ttcgccactt ccctacagag cgcatcccac ggcggcgtcg tgtaggcgtc gaccatgtcg
     3601 gagcgcctgc cgtgggtgac ccactggagt aggtatttgt cggcaccgtc ggcacgagcc
     3661 aggctgatga acgtccggcg agcatcgtga gtccgtctcc gcctgaaccc caggaggtcc
     3721 aggtcggcat gcaggcgacg gaggactccc tggggccgct ggtgcatccc ggtggggctc
     3781 gggagaatca ggtcatcctg gcgcggcgtc cgggcgtaca tccgcgccca gccgtaggcg
     3841 agccaactgg caagcatcgt gctcaaccag gggtggactg gcatctgccg cggcgtgtcc
     3901 gtcttcgtgg tctcgtagac ctctcctacc ttgtggttga aggtcttcgt cacccagagc
     3961 ttccccaagg ggttgacggt gccgtcccag tcccggacct gcagggctga cgcctccccg
     4021 atgcgcaccg ctccgacgaa ggccacggcg tagagcacgc gccgctcaag ggggatgcgc
     4081 tcatccgtaa tcagtgcgac gacctcggcc cgcgagtaga cggccgagtc ccgccatcga
     4141 gggtcagcat cccgaatggc cggaatctcc ccgcggcgct cgcgcaagtc acagggcgag
     4201 cgctccagca gcccgtccac caccgcgtca ctcagcatcg tccgcaccag ggcgtagacg
     4261 tggcgcaccg ttcgcggcgc caggcccttt gcgcgcactt cgcgcaccag tgcgagcacg
     4321 tgccgacgcg tcagcacacc gagttccttg tcgcccaacg agggcagcac gtggtgctgc
     4381 atccgggagc ggtcatccat gaccgagcgc agcgtgacgc gcccctccat ccacttggcg
     4441 gcgtaggcct cgacggtgac gggaccggac gcgccgagtc cctggtcctt ctccgcggcc
     4501 acgcgcttct cggtcgcctt caggacggcg cgcgccttca cttcgtcttc ttcgcccgtg
     4561 ctcgcgtact gccgcgcgcc cgcagcgtcg aaccaccaca tccacaagta gcgagtggcc
     4621 ttcttgcgat agacgttacc cacggcgccg ccctgccttc cgcgccagga tggccgctgc
     4681 cttcgcgtcg gcctcctctt cgggcgtagc cttcgcccgc ggcgacgcga gaaacgcctc
     4741 caactccgac ttcttgacgc gaccacgatg cccagccgtc aggagtccct gcgcctgcca
     4801 gttgcgaatg gtggatgtgc tgacacttgc gagggctgcc gcctcagtct gagtgagcac
     4861 ctcgtccgtc ttctgcgcgg tggtgcccag cacccgccgg acgactcttt cgacgatggc
     4921 ctccacgtca atgtcaatct cactcacgat gactgccccc gttgcaccac gcctgtacgc
     4981 cctgtcaggt aacacggcag actgacaccc cactgcatgc acatgggccg atgcacacgc
     5041 agtggggctg gacgcaggct ccaagtcctc atctgagccc gcatcagaaa cataaatgaa
     5101 acacgcatct aaattcaata cgcgcgatgc aattttcctt gactcggaca cgcgaggaca
     5161 cacggtagtc gactcaagcg caataacacc gactctcccg agtgacggga gtttgctcgc
     5221 agtgttttta cgcaacacaa cgcaatcgca accttgattg caattgcagt ccacgagtcc
     5281 ggacgggtgt atcaagaccg cacgggaggt atcgccgtgc ccagcgtctc gactcgttgc
     5341 tgtcttgagt tgggcgggca cggcgaaccc cggagaacag tgcgccgacg gcgacggtga
     5401 ggagcaccgt cgggcaccgg agtagatgcg cgcccagcgt gccggaacgc gtctactctc
     5461 gaagttgtcg tgtgtcctgg ccgctcaccg tgtgctgccg gtccaggtac tgcgtacttg
     5521 ccaacctgca cccggagacc gggtgctgtc aagtcgggta ctgcgactac ttgaggccaa
     5581 gcgcgcgaag cgctgcggcc cggctgacgc tcagcgtaac ccgccgcgcg tcagcggcgt
     5641 cctgcgtctg ctccacctca acacctgtct cgcggagttg cgccagcagt cgctccgtag
     5701 agcggcgctg gagtcccagc gcggcgccca actccagcgg cgagtgcggg cgctccacca
     5761 ggaggcggag cgccgtcagc gaaagcatgg ggcgcggtgg cgtcgtcacg gcaccaccaa
     5821 ctctgccttg ggccactgtg ccgtgtcgcg ctcgacaagc gcggccgtct ggctccatcc
     5881 ccaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     5941 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     6001 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     6061 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     6121 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     6181 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     6241 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     6301 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     6361 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     6421 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     6481 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gattttttgt
     6541 gatgctcgtc aaggggggcg gaagcctatg gaaaaaacgc cagcaaacgc ggccttttta
     6601 cggtttcctg cccttttgct ggccttttgc tccacatgtt ttttcctgcg ttatcccctg
     6661 attctgtggg ataaaccgta ttacccgcct ttgagtgagc tgataccgct cgccgcagcc
     6721 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     6781 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     6841 gctctgatgc cgcatagtta agccagtata tacactccgc tatcgctacg tgactgggtc
     6901 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     6961 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     7021 tcaccgtcat caccgaaacg cgcgaggcag ccaatacgca aaccgcctct ccccgcgcgt
     7081 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     7141 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     7201 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     7261 tatgaccatg attac
//
