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pMAMneoBlue vector (V004835)

Basic Vector Information

Vector Name:
pMAMneoBlue
Antibiotic Resistance:
Ampicillin
Length:
8475 bp
Type:
Cloning vector
Replication origin:
ori
Source/Author:
Kitts PA.

pMAMneoBlue vector Vector Map

pMAMneoBlue8475 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400MMTVM13 fwdT7 promoterKS primerT3 promoterM13 revlac operatorlac promoterCAP binding siteSV40 poly(A) signalSV40 promoterNeoR/KanRsmall t intronSV40 NLSSV40 poly(A) signalAmpR promoterAmpRoriRSV promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMAMneoBlue vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_29801        8475 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMAMneoBlue, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8475)
  AUTHORS   Kitts PA.
  TITLE     CLONTECH Vectors On Disc version 1.3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8475)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 
            1020 East Meadow Circle, Palo Alto, CA 94303, USA
REFERENCE   3  (bases 1 to 8475)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8475)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     This sequence has been compiled from information in the sequence 
            databases, published literature and other sources, together with 
            partial sequences obtained by CLONTECH. This vector is no longer 
            available from CLONTECH and CLONTECH will not update or revise this 
            sequence.
FEATURES             Location/Qualifiers
     source          1..8475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        99..1410
                     /label=MMTV
                     /note="Mouse mammary tumor virus long terminal repeat
                     (LTR)"
     primer_bind     1693..1709
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1716..1734
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1757..1773)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1836..1854)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1875..1891)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1899..1915)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1923..1953)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1968..1989)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     polyA_signal    2354..2488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2501..2830
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3187..3978
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     intron          4402..4467
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4597..4617
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    5042..5176
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6029..6133
                     /label=AmpR promoter
     CDS             6134..6991
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7165..7753
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        8301..8475
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"

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