LOCUS       40924_29801        8475 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMAMneoBlue, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8475)
  AUTHORS   Kitts PA.
  TITLE     CLONTECH Vectors On Disc version 1.3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8475)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 
            1020 East Meadow Circle, Palo Alto, CA 94303, USA
REFERENCE   3  (bases 1 to 8475)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8475)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     This sequence has been compiled from information in the sequence 
            databases, published literature and other sources, together with 
            partial sequences obtained by CLONTECH. This vector is no longer 
            available from CLONTECH and CLONTECH will not update or revise this 
            sequence.
FEATURES             Location/Qualifiers
     source          1..8475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        99..1410
                     /label=MMTV
                     /note="Mouse mammary tumor virus long terminal repeat
                     (LTR)"
     primer_bind     1693..1709
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1716..1734
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1757..1773)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1836..1854)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1875..1891)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1899..1915)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1923..1953)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1968..1989)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     polyA_signal    2354..2488
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2501..2830
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3187..3978
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     intron          4402..4467
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4597..4617
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    5042..5176
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6029..6133
                     /label=AmpR promoter
     CDS             6134..6991
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7165..7753
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        8301..8475
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gaattccgca ttgcagagat attgtattta agtgcctagc tcgatacaat aaacgccatt
       61 tgaccattca ccacattggt gtgcacctcc aagcttgggc agaaatggtt gaactcccga
      121 gagtgtccta cacctagggg agaagcagcc aaggggttgt ttcccaccaa ggacgacccg
      181 tctgcgcaca aacggatgag cccatcagac aaagacatat tcattctctg ctgcaaactt
      241 ggcatagctc tgctttgcct ggggctattg ggggaagttg cggttcgtgc tcgcagggct
      301 ctcacccttg actcttttaa tagctcttct gtgcaagatt acaatctaaa caattcggag
      361 aactcgacct tcctcctgag gcaaggacca cagccaactt cctcttacaa gccgcatcga
      421 ttttgtcctt cagaaataga aataagaatg cttgctaaaa attatatttt taccaataag
      481 accaatccaa taggtagatt attagttact atgttaagaa atgaatcatt atcttttagt
      541 actattttta ctcaaattca gaagttagaa atgggaatag aaaatagaaa gagacgctca
      601 acctcaattg aagaacaggt gcaaggacta ttgaccacag gcctagaagt aaaaaaggga
      661 aaaaagagtg tttttgtcaa aataggagac aggtggtggc aaccagggac ttatagggga
      721 ccttacatct acagaccaac agatgccccc ttaccatata caggaagata tgacttaaat
      781 tgggataggt gggttacagt caatggctat aaagtgttat atagatccct cccttttcgt
      841 gaaagactcg ccagagctag acctccttgg tgtatgttgt ctcaagaaga aaaagacgac
      901 atgaaacaac aggtacatga ttatatttat ctaggaacag gaatgcactt ttggggaaag
      961 attttccata ccaaggaggg gacagtggct ggactaatag aacattattc tgcaaaaact
     1021 catggcatga gttattatga atagccttta ttggcccaac cttgcggttc ccagggctta
     1081 agtaagtttt tggttacaaa ctgttcttaa aacgaggatg tgagacaagt ggtttcctga
     1141 cttggtttgg tatcaaaggt tctgatctga gctctgagtg ttctattttc ctatgttctt
     1201 ttggaattta tccaaatctt atgtaaatgc ttatgtaaac caagatataa aagagtgctg
     1261 attttttgag taaacttgca acagtcctaa cattcacctc ttgtgtgttt gtgtctgttc
     1321 gccatcccgt ctccgctcgt cacttatcct tcactttcca gagggtcccc ccgcagaccc
     1381 cggcgaccct caggtcggcc gactgcggca gctggcgccc gaacagggac cctcggataa
     1441 gtgacccttg tctctatttc tactatttgg tgtttgtctt gtattgtctc tttcttgtct
     1501 ggctatcatc acaagagcgg aacggactca ccatagggac caagctagcc cggggttcgc
     1561 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     1621 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     1681 agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta tagggcgaat
     1741 tcgatatcaa gcttatcgat accgtcgacc tcgagggggg gcccggtacc cggggatcct
     1801 ctagagtcga cctgcaggca tgcaagcttt tgttcccttt agtgagggtt aatttcgagc
     1861 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     1921 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     1981 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     2041 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcataacagt
     2101 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
     2161 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
     2221 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
     2281 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     2341 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     2401 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     2461 catcaatgta tcttatcatg tctggatccg gctgtggaat gtgtgtcagt tagggtgtgg
     2521 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     2581 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     2641 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     2701 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     2761 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     2821 cttttgcaaa aagcttcacg ctgccgcaag cactcagggc gcaagggctg ctaaaggaag
     2881 cggaacacgt agaaagccag tccgcagaaa cggtgctgac cccggatgaa tgtcagctac
     2941 tgggctatct ggacaaggga aaacgcaagc gcaaagagaa agcaggtagc ttgcagtggg
     3001 cttacatggc gatagctaga ctgggcggtt ttatggacag caagcgaacc ggaattgcca
     3061 gctggggcgc cctctggtaa ggttgggaag ccctgcaaag taaactggat ggctttcttg
     3121 ccgccaagga tctgatggcg caggggatca agatctgatc aagagacagg atgaggatcg
     3181 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     3241 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     3301 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     3361 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     3421 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     3481 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     3541 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     3601 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     3661 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     3721 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     3781 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     3841 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     3901 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     3961 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc
     4021 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     4081 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     4141 tcttcgccca ccccgggctc gatcccctcg cgagttggtt cagctgctgc ctgaggctgg
     4201 acgacctcgc ggagttctac cggcagtgca aatccgtcgg catccaggaa accagcagcg
     4261 gctatccgcg catccatgcc cccgaactgc aggagtgggg aggcacgatg gccgctttgg
     4321 tcccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
     4381 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
     4441 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
     4501 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
     4561 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
     4621 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
     4681 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
     4741 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
     4801 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
     4861 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
     4921 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     4981 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     5041 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5101 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5161 ttatcatgtc tggatcccca ggaagctcct ctgtgtcctc ataaacccta acctcctcta
     5221 cttgagagga cattccaatc ataggctgcc catccaccct ctgtgtcctc ctgttaatta
     5281 ggtcacttaa caaaaaggaa attgggtagg ggtttttcac agaccgcttt ctaagggtaa
     5341 ttttaaaata tctgggaagt cccttccact gctgtgttcc agaagtgttg gtaaacagcc
     5401 cacaaatgtc aacagcagaa acatacaagc tgtcagcttt gcacaagggc ccaacaccct
     5461 gctcatcaag aagcactgtg gttgctgtgt tagtaatgtg caaaacagga ggcacatttt
     5521 ccccacctgt gtaggttcca aaatatctag tgttttcatt tttacttgga tcaggaaccc
     5581 agcactccac tggataagca ttatccttat ccaaaacagc cttgtggtca gtgttcatct
     5641 gctgactgtc aactgtagca ttttttgggg ttacagtttg agcaggatat ttggtcctgt
     5701 agtttgctaa cacaccctgc agctccaaag gttccccacc aacagcaaaa aaatgaaaat
     5761 ttgacccttg aatgggtttt ccagcaccat tttcatgagt tttttgtgtc cctgaatgca
     5821 agtttaacat agcagttacc ccaataacct cagttttaac agtaacagct tcccacatca
     5881 aaatatttcc acaggttaag tcctcattta aattaggcaa aggaattctt gaagacgaaa
     5941 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     6001 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     6061 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     6121 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     6181 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     6241 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     6301 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     6361 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc
     6421 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     6481 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     6541 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     6601 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     6661 tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     6721 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     6781 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     6841 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6901 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6961 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     7021 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     7081 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     7141 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     7201 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     7261 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     7321 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     7381 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     7441 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     7501 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     7561 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     7621 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     7681 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     7741 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     7801 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     7861 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     7921 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     7981 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     8041 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     8101 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     8161 gctgcttcgc gatgtacggg ccagatatac gcgtatctga ggggactagg gtgtgtttag
     8221 gcgaaaagcg gggcttcggt tgtacgcggt taggagtccc ctcaggatat agtagtttcg
     8281 cttttgcata gggaggggga aatgtagtct tatgcaatac acttgtagtc ttgcaacatg
     8341 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg
     8401 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
     8461 attggacgaa ccact
//
