Basic Vector Information
- Vector Name:
- pMAMneo
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8413 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Kitts PA.
pMAMneo vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMAMneo vector Sequence
LOCUS 40924_29796 8413 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector pMAMneo, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8413) AUTHORS Kitts PA. TITLE CLONTECH Vectors On Disc version 1.3 JOURNAL Unpublished REFERENCE 2 (bases 1 to 8413) AUTHORS Kitts PA. TITLE Direct Submission JOURNAL Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East Meadow Circle, Palo Alto, CA 94303, USA REFERENCE 3 (bases 1 to 8413) TITLE Direct Submission REFERENCE 4 (bases 1 to 8413) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East Meadow Circle, Palo Alto, CA 94303, USA" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT This vector can be obtained from CLONTECH Laboratories, Inc., 1020 East Meadow Circle, Palo Alto, CA 94303, USA. To place an order call (415) 424-8222 or (800) 662-2566, extension 1. International customers, please contact your local distributor. For technical information, call (415) 424- 8222 or (800) 662-2566, extension 3. This sequence has been compiled from information in the sequence databases, published literature and other sources, together with partial sequences obtained by CLONTECH; this vector has not been completely sequenced. If you suspect there is an error in this sequence, please contact CLONTECH's Technical Service Department at (415) 424-8222 or (800) 662-2566, extension 3 or E-mail TECH@CLONTECH.COM.'. FEATURES Location/Qualifiers source 1..8413 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 99..1410 /label=MMTV /note="Mouse mammary tumor virus long terminal repeat (LTR)" intron 1652..1717 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 1847..1867 /codon_start=1 /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" /translation="PKKKRKV" polyA_signal 2292..2426 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter 2439..2768 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 3125..3916 /codon_start=1 /label=NeoR/KanR /note="aminoglycoside phosphotransferase" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" intron 4340..4405 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 4535..4555 /codon_start=1 /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" /translation="PKKKRKV" polyA_signal 4980..5114 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter 5967..6071 /label=AmpR promoter CDS 6072..6929 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 7103..7691 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 8239..8413 /label=RSV promoter /note="Rous sarcoma virus enhancer/promoter"
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