LOCUS       40924_29796        8413 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMAMneo, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8413)
  AUTHORS   Kitts PA.
  TITLE     CLONTECH Vectors On Disc version 1.3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8413)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 
            1020 East Meadow Circle, Palo Alto, CA 94303, USA
REFERENCE   3  (bases 1 to 8413)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8413)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020 
            East Meadow Circle, Palo Alto, CA 94303, USA. To place an order call
            (415) 424-8222 or (800) 662-2566, extension 1. International 
            customers, please contact your local distributor.  For technical 
            information, call (415) 424- 8222 or (800) 662-2566, extension 3. 
            This sequence has been compiled from information in the sequence 
            databases, published literature and other sources, together with 
            partial sequences obtained by CLONTECH; this vector has not been 
            completely sequenced. If you suspect there is an error in this 
            sequence, please contact CLONTECH's Technical Service Department at 
            (415) 424-8222 or (800) 662-2566, extension 3 or E-mail 
            TECH@CLONTECH.COM.'.
FEATURES             Location/Qualifiers
     source          1..8413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        99..1410
                     /label=MMTV
                     /note="Mouse mammary tumor virus long terminal repeat
                     (LTR)"
     intron          1652..1717
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1847..1867
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    2292..2426
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2439..2768
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3125..3916
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     intron          4340..4405
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4535..4555
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    4980..5114
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5967..6071
                     /label=AmpR promoter
     CDS             6072..6929
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7103..7691
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        8239..8413
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gaattccgca ttgcagagat attgtattta agtgcctagc tcgatacaat aaacgccatt
       61 tgaccattca ccacattggt gtgcacctcc aagcttgggc agaaatggtt gaactcccga
      121 gagtgtccta cacctagggg agaagcagcc aaggggttgt ttcccaccaa ggacgacccg
      181 tctgcgcaca aacggatgag cccatcagac aaagacatat tcattctctg ctgcaaactt
      241 ggcatagctc tgctttgcct ggggctattg ggggaagttg cggttcgtgc tcgcagggct
      301 ctcacccttg actcttttaa tagctcttct gtgcaagatt acaatctaaa caattcggag
      361 aactcgacct tcctcctgag gcaaggacca cagccaactt cctcttacaa gccgcatcga
      421 ttttgtcctt cagaaataga aataagaatg cttgctaaaa attatatttt taccaataag
      481 accaatccaa taggtagatt attagttact atgttaagaa atgaatcatt atcttttagt
      541 actattttta ctcaaattca gaagttagaa atgggaatag aaaatagaaa gagacgctca
      601 acctcaattg aagaacaggt gcaaggacta ttgaccacag gcctagaagt aaaaaaggga
      661 aaaaagagtg tttttgtcaa aataggagac aggtggtggc aaccagggac ttatagggga
      721 ccttacatct acagaccaac agatgccccc ttaccatata caggaagata tgacttaaat
      781 tgggataggt gggttacagt caatggctat aaagtgttat atagatccct cccttttcgt
      841 gaaagactcg ccagagctag acctccttgg tgtatgttgt ctcaagaaga aaaagacgac
      901 atgaaacaac aggtacatga ttatatttat ctaggaacag gaatgcactt ttggggaaag
      961 attttccata ccaaggaggg gacagtggct ggactaatag aacattattc tgcaaaaact
     1021 catggcatga gttattatga atagccttta ttggcccaac cttgcggttc ccagggctta
     1081 agtaagtttt tggttacaaa ctgttcttaa aacgaggatg tgagacaagt ggtttcctga
     1141 cttggtttgg tatcaaaggt tctgatctga gctctgagtg ttctattttc ctatgttctt
     1201 ttggaattta tccaaatctt atgtaaatgc ttatgtaaac caagatataa aagagtgctg
     1261 attttttgag taaacttgca acagtcctaa cattcacctc ttgtgtgttt gtgtctgttc
     1321 gccatcccgt ctccgctcgt cacttatcct tcactttcca gagggtcccc ccgcagaccc
     1381 cggcgaccct caggtcggcc gactgcggca gctggcgccc gaacagggac cctcggataa
     1441 gtgacccttg tctctatttc tactatttgg tgtttgtctt gtattgtctc tttcttgtct
     1501 ggctatcatc acaagagcgg aacggactca ccatagggac caagctagcc cggggtcgac
     1561 cggtcgacct cgagggatct ttgtgaagga accttacttc tgtggtgtga cataattgga
     1621 caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg
     1681 tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga
     1741 atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc
     1801 atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag
     1861 aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt
     1921 gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact
     1981 gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta
     2041 taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa
     2101 ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata
     2161 tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt
     2221 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     2281 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     2341 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     2401 tcaatgtatc ttatcatgtc tggatccggc tgtggaatgt gtgtcagtta gggtgtggaa
     2461 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     2521 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     2581 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     2641 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     2701 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     2761 tttgcaaaaa gcttcacgct gccgcaagca ctcagggcgc aagggctgct aaaggaagcg
     2821 gaacacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg tcagctactg
     2881 ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt gcagtgggct
     2941 tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg aattgccagc
     3001 tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc
     3061 gccaaggatc tgatggcgca ggggatcaag atctgatcaa gagacaggat gaggatcgtt
     3121 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     3181 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     3241 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     3301 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     3361 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     3421 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     3481 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     3541 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     3601 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
     3661 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     3721 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     3781 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     3841 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     3901 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
     3961 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     4021 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     4081 ttcgcccacc ccgggctcga tcccctcgcg agttggttca gctgctgcct gaggctggac
     4141 gacctcgcgg agttctaccg gcagtgcaaa tccgtcggca tccaggaaac cagcagcggc
     4201 tatccgcgca tccatgcccc cgaactgcag gagtggggag gcacgatggc cgctttggtc
     4261 ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac
     4321 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact
     4381 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg
     4441 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga
     4501 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga
     4561 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag
     4621 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa
     4681 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat
     4741 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa
     4801 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag
     4861 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     4921 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     4981 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5041 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     5101 atcatgtctg gatccccagg aagctcctct gtgtcctcat aaaccctaac ctcctctact
     5161 tgagaggaca ttccaatcat aggctgccca tccaccctct gtgtcctcct gttaattagg
     5221 tcacttaaca aaaaggaaat tgggtagggg tttttcacag accgctttct aagggtaatt
     5281 ttaaaatatc tgggaagtcc cttccactgc tgtgttccag aagtgttggt aaacagccca
     5341 caaatgtcaa cagcagaaac atacaagctg tcagctttgc acaagggccc aacaccctgc
     5401 tcatcaagaa gcactgtggt tgctgtgtta gtaatgtgca aaacaggagg cacattttcc
     5461 ccacctgtgt aggttccaaa atatctagtg ttttcatttt tacttggatc aggaacccag
     5521 cactccactg gataagcatt atccttatcc aaaacagcct tgtggtcagt gttcatctgc
     5581 tgactgtcaa ctgtagcatt ttttggggtt acagtttgag caggatattt ggtcctgtag
     5641 tttgctaaca caccctgcag ctccaaaggt tccccaccaa cagcaaaaaa atgaaaattt
     5701 gacccttgaa tgggttttcc agcaccattt tcatgagttt tttgtgtccc tgaatgcaag
     5761 tttaacatag cagttacccc aataacctca gttttaacag taacagcttc ccacatcaaa
     5821 atatttccac aggttaagtc ctcatttaaa ttaggcaaag gaattcttga agacgaaagg
     5881 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     5941 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6001 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6061 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6121 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6181 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6241 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6301 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6361 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6421 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     6481 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     6541 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6601 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6661 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6721 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6781 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6841 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6901 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6961 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7021 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7081 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7141 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7201 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     7261 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7321 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7381 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7441 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     7501 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     7561 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7621 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7681 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     7741 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7801 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7861 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     7921 accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     7981 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     8041 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     8101 tgcttcgcga tgtacgggcc agatatacgc gtatctgagg ggactagggt gtgtttaggc
     8161 gaaaagcggg gcttcggttg tacgcggtta ggagtcccct caggatatag tagtttcgct
     8221 tttgcatagg gagggggaaa tgtagtctta tgcaatacac ttgtagtctt gcaacatggt
     8281 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt
     8341 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     8401 tggacgaacc act
//
