pMAB143 vector (V004848)

LOCUS       40924_29641        4861 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMAB143, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Stewart DIH., Wiersma EJ, Druar C, Saini SS, Cossitt MA.
  TITLE     Analysis of the expressed repertoire of Macaca fascicularis V heavy 
            genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Stewart DIH., Wiersma EJ, Druar C, Saini SS, Cossitt MA.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2005) Cangene Corporation, 3403 American Dr., 
            Mississauga, ON L4V 1T4, Canada
REFERENCE   3  (bases 1 to 4861)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4861)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-MAY-2005) Cangene Corporation, 3403 American Dr., Mississauga, 
            ON L4V 1T4, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     sig_peptide     34..96
                     /label=pelB signal sequence
                     /note="leader peptide for secretion"
     CDS             424..1644
                     /codon_start=1
                     /label=M13 gene III
                     /note="pIII"
                     /translation="AASTVESCLAKPHTENSFTNVWKDDKTLDRYANYEGCLWNATGVV
                     VCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGGTKPPEYGDTPIPGYTYI
                     NPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQGALTVYTGTVTQGTDPV
                     KTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQGQSSDLPQPPVNAGGGS
                     GGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKMANANKGAMTENADENAL
                     QSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGSNSQMAQVGDGDNSPLMN
                     NFRQYLPSLPQSVECRPYVFGAGKPYEFSIDCDKINLFRGVFAFLLYVATFMYVFSTFA
                     NILRNKES"
     CDS             1657..1674
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     primer_bind     complement(1719..1735)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1948..2403
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2685..2789
                     /label=AmpR promoter
     CDS             2790..3647
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3821..4409
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4697..4718
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4733..4763
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4771..4787
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     sig_peptide     join(4807..4861,1..11)
                     /label=pelB signal sequence
                     /note="leader peptide for secretion"