Basic Vector Information
- Vector Name:
- pMAB143
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4861 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Stewart DIH., Wiersma EJ, Druar C, Saini SS, Cossitt MA.
pMAB143 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMAB143 vector Sequence
LOCUS 40924_29641 4861 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pMAB143, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4861)
AUTHORS Stewart DIH., Wiersma EJ, Druar C, Saini SS, Cossitt MA.
TITLE Analysis of the expressed repertoire of Macaca fascicularis V heavy
genes
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4861)
AUTHORS Stewart DIH., Wiersma EJ, Druar C, Saini SS, Cossitt MA.
TITLE Direct Submission
JOURNAL Submitted (16-MAY-2005) Cangene Corporation, 3403 American Dr.,
Mississauga, ON L4V 1T4, Canada
REFERENCE 3 (bases 1 to 4861)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4861)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(16-MAY-2005) Cangene Corporation, 3403 American Dr., Mississauga,
ON L4V 1T4, Canada"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4861
/mol_type="other DNA"
/organism="synthetic DNA construct"
sig_peptide 34..96
/label=pelB signal sequence
/note="leader peptide for secretion"
CDS 424..1644
/codon_start=1
/label=M13 gene III
/note="pIII"
/translation="AASTVESCLAKPHTENSFTNVWKDDKTLDRYANYEGCLWNATGVV
VCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGGTKPPEYGDTPIPGYTYI
NPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQGALTVYTGTVTQGTDPV
KTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQGQSSDLPQPPVNAGGGS
GGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKMANANKGAMTENADENAL
QSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGSNSQMAQVGDGDNSPLMN
NFRQYLPSLPQSVECRPYVFGAGKPYEFSIDCDKINLFRGVFAFLLYVATFMYVFSTFA
NILRNKES"
CDS 1657..1674
/codon_start=1
/label=6xHis
/note="6xHis affinity tag"
/translation="HHHHHH"
primer_bind complement(1719..1735)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 1948..2403
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2685..2789
/label=AmpR promoter
CDS 2790..3647
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3821..4409
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 4697..4718
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4733..4763
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4771..4787
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
sig_peptide join(4807..4861,1..11)
/label=pelB signal sequence
/note="leader peptide for secretion"
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