LOCUS       40924_29641        4861 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMAB143, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Stewart DIH., Wiersma EJ, Druar C, Saini SS, Cossitt MA.
  TITLE     Analysis of the expressed repertoire of Macaca fascicularis V heavy 
            genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Stewart DIH., Wiersma EJ, Druar C, Saini SS, Cossitt MA.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2005) Cangene Corporation, 3403 American Dr., 
            Mississauga, ON L4V 1T4, Canada
REFERENCE   3  (bases 1 to 4861)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4861)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-MAY-2005) Cangene Corporation, 3403 American Dr., Mississauga, 
            ON L4V 1T4, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     sig_peptide     34..96
                     /label=pelB signal sequence
                     /note="leader peptide for secretion"
     CDS             424..1644
                     /codon_start=1
                     /label=M13 gene III
                     /note="pIII"
                     /translation="AASTVESCLAKPHTENSFTNVWKDDKTLDRYANYEGCLWNATGVV
                     VCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGGTKPPEYGDTPIPGYTYI
                     NPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQGALTVYTGTVTQGTDPV
                     KTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQGQSSDLPQPPVNAGGGS
                     GGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKMANANKGAMTENADENAL
                     QSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGSNSQMAQVGDGDNSPLMN
                     NFRQYLPSLPQSVECRPYVFGAGKPYEFSIDCDKINLFRGVFAFLLYVATFMYVFSTFA
                     NILRNKES"
     CDS             1657..1674
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     primer_bind     complement(1719..1735)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      1948..2403
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2685..2789
                     /label=AmpR promoter
     CDS             2790..3647
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3821..4409
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4697..4718
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4733..4763
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4771..4787
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     sig_peptide     join(4807..4861,1..11)
                     /label=pelB signal sequence
                     /note="leader peptide for secretion"
ORIGIN
        1 cggccatggc tctagagtaa ggaggcagtc ataatgaagt accttttgcc aacggctgcc
       61 gctggcttgt tattgctcgc ggcacagccg gcaattgggc gcgcctagtc gaccaagggc
      121 ccatcggtct tccccctggc accctcctcc aagagcacct ctgggggcac agcggccctg
      181 ggctgcctgg tcaaggacta cttccccgaa ccggtgacgg tgtcgtggaa ctcaggcgcc
      241 ctgaccagcg gcgtgcacac cttcccggct gtcctacagt cctcaggact ctactccctc
      301 agcagcgtgg tgaccgtgcc ctccagcagc ttgggcaccc agacctacat ctgcaacgtg
      361 aatcacaagc ccagcaacac caaggtggac aagaaagttg agcccaaatc ttgtgacaaa
      421 gcggccgcta gcactgttga aagttgttta gcaaaacctc atacagaaaa ttcatttact
      481 aacgtctgga aagacgacaa aactttagat cgttacgcta actatgaggg ctgtctgtgg
      541 aatgctacgg gcgttgtggt ttgcactggt gacgaaactc agtgttacgg tacatgggtt
      601 cctattgggc ttgctatccc tgaaaatgag ggtggtggct ctgagggtgg cggttctgag
      661 ggtggcggtt ctgagggtgg cggtactaaa cctccagagt acggtgatac acctattccg
      721 ggctatactt atatcaaccc tctcgacggc acttatccgc ctggtactga gcaaaacccc
      781 gctaatccta atccttctct tgaggagtct cagcctctta atactttcat gtttcagaat
      841 aataggttcc gaaataggca gggtgcatta actgtttata cgggcactgt tactcaaggc
      901 actgaccccg ttaaaactta ttaccagtac actcctgtat catcaaaagc catgtatgac
      961 gcttactgga acggtaaatt cagagactgc gctttccatt ctggctttaa tgaggatcca
     1021 ttcgtttgtg aatatcaagg ccaatcgtct gacctgcctc aacctcctgt caatgctggc
     1081 ggcggctctg gtggtggttc tggtggcggc tctgagggtg gcggctctga gggtggcggt
     1141 tctgagggtg gcggctctga gggtggcggt tccggtggcg gctccggttc cggtgatttt
     1201 gattatgaaa aaatggcaaa cgctaataag ggggctatga ccgaaaatgc cgatgaaaac
     1261 gcgctacagt ctgacgctaa aggcaaactt gattctgtcg ctactgatta cggtgctgct
     1321 atcgatggtt tcattggtga cgtttccggc cttgctaatg gtaatggtgc tactggtgat
     1381 tttgctggct ctaattccca aatggctcaa gtcggtgacg gtgataattc acctttaatg
     1441 aataatttcc gtcaatattt accttctttg cctcagtcgg ttgaatgtcg cccttatgtc
     1501 tttggcgctg gtaaaccata tgaattttct attgattgtg acaaaataaa cttattccgt
     1561 ggtgtctttg cgtttctttt atatgttgcc acctttatgt atgtattttc gacgtttgct
     1621 aacatactgc gtaataagga gtcttaataa gctagccatc accaccatca tcactaataa
     1681 tgaaagcccg cctaatgagc gggctttttt ttgaattcac tggccgtcgt tttacaacgt
     1741 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     1801 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     1861 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     1921 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
     1981 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     2041 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     2101 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     2161 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     2221 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     2281 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     2341 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
     2401 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     2461 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     2521 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     2581 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     2641 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     2701 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2761 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2821 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2881 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2941 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     3001 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     3061 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3121 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3181 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3241 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3301 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     3361 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3421 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3481 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     3541 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3601 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3661 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3721 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3781 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3841 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3901 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3961 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4021 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4081 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4141 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4201 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4261 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4321 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4381 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4441 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4501 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     4561 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     4621 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     4681 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     4741 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     4801 acagctatga aatacctatt gcctacggca gccgctggat tgttattact cgcggcccag
     4861 c
//
