Basic Vector Information
- Vector Name:
- pMAB136
- Antibiotic Resistance:
- Ampicillin
- Length:
- 6740 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Soltes G, Hust M, Ng KK, Bansal A, Field J, Stewart DI, Dubel S, Cha S, Wiersma EJ.
pMAB136 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMAB136 vector Sequence
LOCUS 40924_29636 6740 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pMAB136, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6740)
AUTHORS Soltes G, Hust M, Ng KK, Bansal A, Field J, Stewart DI, Dubel S, Cha
S, Wiersma EJ.
TITLE On the influence of vector design on antibody phage display
JOURNAL J. Biotechnol. 127 (4), 626-637 (2007)
PUBMED 16996161
REFERENCE 2 (bases 1 to 6740)
AUTHORS Soltes GA, EngBerg J.
TITLE Direct Submission
JOURNAL Submitted (07-JUL-2005) Biology, Cangene Corporation, 3403 American
Drive, Mississauga, ON L4V1T4, Canada
REFERENCE 3 (bases 1 to 6740)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 6740)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J.
Biotechnol."; date: "2007"; volume: "127"; issue: "4"; pages:
"626-637"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(07-JUL-2005) Biology, Cangene Corporation, 3403 American Drive,
Mississauga, ON L4V1T4, Canada"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6740
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 577..598
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 613..643
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 651..667
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
sig_peptide 687..752
/label=pelB signal sequence
/note="leader peptide for secretion"
CDS 1074..1391
/codon_start=1
/label=hIg-kappa-CL
/note="Human immunoglobulin kappa light chain constant
region"
/translation="TVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNA
LQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG
EC"
sig_peptide 1419..1484
/label=pelB signal sequence
/note="leader peptide for secretion"
CDS 2172..3398
/codon_start=1
/label=M13 gene III
/note="pIII"
/translation="SKDIRTVESCLAKPHTENSFTNVWKDDKTLDRYANYEGCLWNATG
VVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGGTKPPEYGDTPIPGYT
YINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQGALTVYTGTVTQGTD
PVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQGQSSDLPQPPVNAGG
GSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKMANANKGAMTENADEN
ALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGSNSQMAQVGDGDNSPL
MNNFRQYLPSLPQSVECRPYVFGAGKPYEFSIDCDKINLFRGVFAFLLYVATFMYVFST
FANILRNKES"
CDS 3453..3470
/codon_start=1
/label=6xHis
/note="6xHis affinity tag"
/translation="HHHHHH"
promoter complement(3495..3513)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3520..3536)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 3681..4136
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4165..4269
/label=AmpR promoter
CDS 4270..5127
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5301..5889
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 6080..6157
/label=lacI promoter
CDS join(6158..6740,1..497)
/codon_start=1
/label=lacI
/note="lac repressor"
/translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
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