LOCUS       40924_29636        6740 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pMAB136, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Soltes G, Hust M, Ng KK, Bansal A, Field J, Stewart DI, Dubel S, Cha
            S, Wiersma EJ.
  TITLE     On the influence of vector design on antibody phage display
  JOURNAL   J. Biotechnol. 127 (4), 626-637 (2007)
  PUBMED    16996161
REFERENCE   2  (bases 1 to 6740)
  AUTHORS   Soltes GA, EngBerg J.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2005) Biology, Cangene Corporation, 3403 American 
            Drive, Mississauga, ON L4V1T4, Canada
REFERENCE   3  (bases 1 to 6740)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6740)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biotechnol."; date: "2007"; volume: "127"; issue: "4"; pages: 
            "626-637"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (07-JUL-2005) Biology, Cangene Corporation, 3403 American Drive, 
            Mississauga, ON L4V1T4, Canada"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    577..598
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        613..643
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    651..667
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     sig_peptide     687..752
                     /label=pelB signal sequence
                     /note="leader peptide for secretion"
     CDS             1074..1391
                     /codon_start=1
                     /label=hIg-kappa-CL
                     /note="Human immunoglobulin kappa light chain constant
                     region"
                     /translation="TVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNA
                     LQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRG
                     EC"
     sig_peptide     1419..1484
                     /label=pelB signal sequence
                     /note="leader peptide for secretion"
     CDS             2172..3398
                     /codon_start=1
                     /label=M13 gene III
                     /note="pIII"
                     /translation="SKDIRTVESCLAKPHTENSFTNVWKDDKTLDRYANYEGCLWNATG
                     VVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGGTKPPEYGDTPIPGYT
                     YINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQGALTVYTGTVTQGTD
                     PVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQGQSSDLPQPPVNAGG
                     GSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKMANANKGAMTENADEN
                     ALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGSNSQMAQVGDGDNSPL
                     MNNFRQYLPSLPQSVECRPYVFGAGKPYEFSIDCDKINLFRGVFAFLLYVATFMYVFST
                     FANILRNKES"
     CDS             3453..3470
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     promoter        complement(3495..3513)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3520..3536)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3681..4136
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4165..4269
                     /label=AmpR promoter
     CDS             4270..5127
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5301..5889
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        6080..6157
                     /label=lacI promoter
     CDS             join(6158..6740,1..497)
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg
       61 aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg
      121 agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc
      181 gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg
      241 ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc
      301 tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg
      361 gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc
      421 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
      481 gactggaaag cgggcagtga gctcgcggta cccgataaaa gcggcttcct gacaggaggc
      541 cgttttgttt tgcagcccac ctgagctcaa cgcaattaat gtgagttagc tcactcatta
      601 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      661 ataacaattt cacacaggaa acagctatga aatacctatt gcctacggca gccgctggat
      721 tgttattact cgcggcccag ccggccatgg ctgacatcca gatgacccag tctccatcct
      781 ccctgtctgc atctgtagga gacagagtca tcatcacttg ccgggcaagt cagagtatta
      841 gcacctattt aaattggtat cagcagaaac cagggaaagc ccctaaactc ctgatctatt
      901 atgcaaccaa tttgcaaagt ggggtcccat caaggttcag tggcagtgga tctgggacag
      961 atttcactct caccatcagc agtctgcaac ctgaagattt tgcgacttat tattgtcaac
     1021 agagttccaa caccgtcact ttcggccctg ggaccaaagt ggatatgaag cgaactgtgg
     1081 ctgcaccatc tgtcttcatc ttcccgccat ctgatgagca gttgaaatct ggaactgcct
     1141 ctgttgtgtg cctgctgaat aacttctatc ccagagaggc caaagtacag tggaaggtgg
     1201 ataacgccct ccaatcgggt aactcccagg agagtgtcac agagcaggac agcaaggaca
     1261 gcacctacag cctcagcagc accctgacgc tgagcaaagc agactacgag aaacacaaag
     1321 tctacgcctg cgaagtcacc catcagggcc tgagttcgcc cgtcacaaag agcttcaaca
     1381 ggggagagtg ttaattctag agtaaggagg cagtcataat gaagtacctt ttgccaacgg
     1441 ctgccgctgg cttgttattg ctcgcggcac agccggcaat tgcccaggtg cagctggtgc
     1501 agtctggggg aggcttggta cagcctgggg ggtccgtgag actctcctgt gcagcctctg
     1561 gattcagttt tagcagctat gccatgagct gggtccgcca ggctccaggg atggggctgg
     1621 agtgggtcgc ggctattagt gctagaggaa ctaccacata ttatgcagac tccgtgacgg
     1681 gccgattgac catctccaga gacaattcca tgaacacgct atatctgcac ttgaacagcc
     1741 tgagagccga ggacacggcc gtttattact gtgcgaaagc gggaaaacag tggctggccc
     1801 actactactt tgactcctgg ggccagggaa ccctggtcac cgtctcctca gcctccacca
     1861 agggcccatc ggtcttcccc ctggcaccct cctccaagag cacctctggg ggcacagcgg
     1921 ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg tggaactcag
     1981 gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca ggactctact
     2041 ccctcagcag cgtggtgact gtgccctcca gcagcttggg cacccagacc tacatctgca
     2101 acgtgaatca caagcccagc aacaccaagg tggacaagaa agcagagccc aaatcttgtg
     2161 cggccgcagg ctctaaagat atcagaactg ttgaaagttg tttagcaaaa cctcatacag
     2221 aaaattcatt tactaacgtc tggaaagacg acaaaacttt agatcgttac gctaactatg
     2281 agggctgtct gtggaatgct acaggcgttg tggtttgtac tggtgacgaa actcagtgtt
     2341 acggtacatg ggttcctatt gggcttgcta tccctgaaaa tgagggtggt ggctctgagg
     2401 gtggcggttc tgagggtggc ggttctgagg gtggcggtac taaacctcct gagtacggtg
     2461 atacacctat tccgggctat acttatatca accctctcga cggcacttat ccgcctggta
     2521 ctgagcaaaa ccccgctaat cctaatcctt ctcttgagga gtctcagcct cttaatactt
     2581 tcatgtttca gaataatagg ttccgaaata ggcagggtgc attaactgtt tatacgggca
     2641 ctgttactca aggcactgac cccgttaaaa cttattacca gtacactcct gtatcatcaa
     2701 aagccatgta tgacgcttac tggaacggta aattcagaga ctgcgctttc cattctggct
     2761 ttaatgagga tccattcgtt tgtgaatatc aaggccaatc gtctgacctg cctcaacctc
     2821 ctgtcaatgc tggcggcggc tctggtggtg gttctggtgg cggctctgag ggtggcggct
     2881 ctgagggtgg cggttctgag ggtggcggct ctgagggtgg cggttccggt ggcggctccg
     2941 gttccggtga ttttgattat gaaaaaatgg caaacgctaa taagggggct atgaccgaaa
     3001 atgccgatga aaacgcgcta cagtctgacg ctaaaggcaa acttgattct gtcgctactg
     3061 attacggtgc tgctatcgat ggtttcattg gtgacgtttc cggccttgct aatggtaatg
     3121 gtgctactgg tgattttgct ggctctaatt cccaaatggc tcaagtcggt gacggtgata
     3181 attcaccttt aatgaataat ttccgtcaat atttaccttc tttgcctcag tcggttgaat
     3241 gtcgccctta tgtctttggc gctggtaaac catatgaatt ttctattgat tgtgacaaaa
     3301 taaacttatt ccgtggtgtc tttgcgtttc ttttatatgt tgccaccttt atgtatgtat
     3361 tttcgacgtt tgctaacata ctgcgtaata aggagtctta ataagaattc ctgcagcccg
     3421 ggggatccac tagctagttc tagacggccg cccatcatca tcatcatcat tgatcaccgc
     3481 ggtggccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg
     3541 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     3601 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     3661 cctgaatggc gaatggcgcg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     3721 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     3781 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     3841 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     3901 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     3961 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     4021 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     4081 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caatttccca
     4141 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     4201 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     4261 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     4321 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     4381 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     4441 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     4501 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     4561 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     4621 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     4681 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     4741 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     4801 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     4861 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     4921 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     4981 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5041 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     5101 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     5161 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     5221 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     5281 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     5341 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     5401 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     5461 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     5521 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     5581 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     5641 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     5701 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     5761 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     5821 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     5881 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     5941 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6001 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6061 gaagcggaaa ctagctagtg acaccatcga atggcgcaaa acctttcgcg gtatggcatg
     6121 atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgttatacga
     6181 tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag
     6241 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
     6301 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
     6361 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
     6421 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa
     6481 agcggcggtg cacaatcttc tcgcgcagag agtcagtggg ctgatcatta actatccgct
     6541 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct
     6601 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg
     6661 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcaggtcc
     6721 gctgtcctct gtctcggcgc
//
