Basic Vector Information
- Vector Name:
- pMAA-Red
- Antibiotic Resistance:
- Kanamycin
- Length:
- 9713 bp
- Type:
- Binary cloning vector
- Replication origin:
- ori
- Host:
- Plants
- Source/Author:
- Ali MA, Shah KH, Bohlmann H.
- Promoter:
- CaMV 35S
pMAA-Red vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMAA-Red vector Sequence
LOCUS 40924_29631 9713 bp DNA circular SYN 18-DEC-2018
DEFINITION Binary cloning vector pMAA-Red, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9713)
AUTHORS Ali MA, Shah KH, Bohlmann H.
TITLE pMAA-Red: Binary cloning vector
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9713)
AUTHORS Ali MA, Shah KH, Bohlmann H.
TITLE Direct Submission
JOURNAL Submitted (08-MAY-2012) Department of Crop Sciences, Division of
Plant Protection, University of Natural Resources and Life Sciences,
Konrad Lorenz Strasse 24, Tulln, Lower Austria 3430, Austria
REFERENCE 3 (bases 1 to 9713)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9713)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(08-MAY-2012) Department of Crop Sciences, Division of Plant
Protection, University of Natural Resources and Life Sciences,
Konrad Lorenz Strasse 24, Tulln, Lower Austria 3430, Austria"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9713
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 404..1030
/label=pVS1 StaA
/note="stability protein from the Pseudomonas plasmid pVS1
(Heeb et al., 2000)"
CDS 1462..2532
/label=pVS1 RepA
/note="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
rep_origin 2601..2795
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
rep_origin complement(3148..3736)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3825..4616)
/label=KanR
/note="aminoglycoside phosphotransferase"
misc_feature 5045..5069
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
regulatory 5138..5530
/label=PDF2.1 from Arabidopsis plant defensin 2.1
/note="PDF2.1 from Arabidopsis plant defensin 2.1"
/regulatory_class="promoter"
CDS 5531..6205
/label=DsRed2
/note="improved tetrameric variant of DsRed fluorescent
protein"
terminator 6209..6461
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 6465..6500
/note="polylinker; multiple cloning site"
promoter 6992..7337
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
misc_feature 7357..7412
/label=TMV Omega
/note="translational enhancer from the tobacco mosaic virus
5'-leader sequence (Gallie et al., 1988)"
CDS 7416..9224
/label=GUS
/note="beta-glucuronidase"
regulatory 9279..9518
/label=CaMV_35S
/note="CaMV_35S"
/regulatory_class="terminator"
misc_feature 9575..9599
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
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