pMAA-Red vector (V004850)

LOCUS       40924_29631        9713 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary cloning vector pMAA-Red, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Ali MA, Shah KH, Bohlmann H.
  TITLE     pMAA-Red: Binary cloning vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9713)
  AUTHORS   Ali MA, Shah KH, Bohlmann H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2012) Department of Crop Sciences, Division of 
            Plant Protection, University of Natural Resources and Life Sciences,
            Konrad Lorenz Strasse 24, Tulln, Lower Austria 3430, Austria
REFERENCE   3  (bases 1 to 9713)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9713)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-MAY-2012) Department of Crop Sciences, Division of Plant 
            Protection, University of Natural Resources and Life Sciences, 
            Konrad Lorenz Strasse 24, Tulln, Lower Austria 3430, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             404..1030
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             1462..2532
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      2601..2795
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      complement(3148..3736)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3825..4616)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    5045..5069
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      5138..5530
                     /label=PDF2.1 from Arabidopsis plant defensin 2.1
                     /note="PDF2.1 from Arabidopsis plant defensin 2.1"
                     /regulatory_class="promoter"
     CDS             5531..6205
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     terminator      6209..6461
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6465..6500
                     /note="polylinker; multiple cloning site"
     promoter        6992..7337
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    7357..7412
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             7416..9224
                     /label=GUS
                     /note="beta-glucuronidase"
     regulatory      9279..9518
                     /label=CaMV_35S
                     /note="CaMV_35S"
                     /regulatory_class="terminator"
     misc_feature    9575..9599
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"