LOCUS       40924_29631        9713 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary cloning vector pMAA-Red, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Ali MA, Shah KH, Bohlmann H.
  TITLE     pMAA-Red: Binary cloning vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9713)
  AUTHORS   Ali MA, Shah KH, Bohlmann H.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2012) Department of Crop Sciences, Division of 
            Plant Protection, University of Natural Resources and Life Sciences,
            Konrad Lorenz Strasse 24, Tulln, Lower Austria 3430, Austria
REFERENCE   3  (bases 1 to 9713)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9713)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-MAY-2012) Department of Crop Sciences, Division of Plant 
            Protection, University of Natural Resources and Life Sciences, 
            Konrad Lorenz Strasse 24, Tulln, Lower Austria 3430, Austria"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9713
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             404..1030
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             1462..2532
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      2601..2795
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      complement(3148..3736)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3825..4616)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     misc_feature    5045..5069
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     regulatory      5138..5530
                     /label=PDF2.1 from Arabidopsis plant defensin 2.1
                     /note="PDF2.1 from Arabidopsis plant defensin 2.1"
                     /regulatory_class="promoter"
     CDS             5531..6205
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     terminator      6209..6461
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    6465..6500
                     /note="polylinker; multiple cloning site"
     promoter        6992..7337
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     misc_feature    7357..7412
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             7416..9224
                     /label=GUS
                     /note="beta-glucuronidase"
     regulatory      9279..9518
                     /label=CaMV_35S
                     /note="CaMV_35S"
                     /regulatory_class="terminator"
     misc_feature    9575..9599
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 agtactttaa agtactttaa agtactttaa agtactttga tccaacccct ccgctgctat
       61 agtgcagtcg gctccggaga atgaacgcca agaggaacaa gcatgaaacc gtaccaggac
      121 ggccaggacg aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcgtcgggt
      181 acgtgttcga gccgcccgcg cacgtctcaa ccgtgcggct gcatgaaatc ctggccggtt
      241 tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc
      301 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
      361 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
      421 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
      481 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
      541 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
      601 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
      661 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
      721 gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat
      781 tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac
      841 gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc
      901 ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga
      961 atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc
     1021 aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt
     1081 gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg
     1141 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg
     1201 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta
     1261 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg
     1321 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac
     1381 gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac
     1441 ccccaagccc gaggaatcgg cgtgagcggt cgcaaaccat ccggcccggt acaaatcggc
     1501 gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa
     1561 cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag caaccgctga tcgaatccgc
     1621 aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc
     1681 gacgagcaac cagatttttt cgttccgatg ctctatgacg tgggcacccg cgatagtcgc
     1741 agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg
     1801 atccgctacg agcttccaga cgggcacgta gaggtttccg cagggccggc cggcatggcc
     1861 agtgtgtggg attacgacct ggtactgatg gcggtttccc atctaaccga atccatgaac
     1921 cgataccggg aagggaaggg agacaaaccc ggccgcgtgt tccgtccaca cgttgcggac
     1981 gtactcaagt tctgccggcg agccgatggc ggaaagcaga aagacgacct ggtagaaacc
     2041 tgcattcggt taaacaccac gcacgttgcc atgcagcgta cgaagaaggc caagaacggc
     2101 cgcctggtga cggtatccga gggtgaagcc ttgattagcc gctacaagat cgtaaagagc
     2161 gaaaccgggc ggccggagta catcgagatc gagctagctg attggatgta ccgcgagatc
     2221 acagaaggca agaacccgga cgtgctgacg gttcaccccg attacttttt gatcgatccc
     2281 ggcatcggcc gttttctcta ccgcctggca cgccgcgccg caggcaaggc agaagccaga
     2341 tggttgttca agacgatcta cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt
     2401 ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg agtacgattt gaaggaggag
     2461 gcggggcagg ctggcccgat cctagtcatg cgctaccgca acctgatcga gggcgaagca
     2521 tccgccggtt cctaatgtac ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa
     2581 ggtcgaaaag gtctctttcc tgtggatagc acgtacattg ggaacccaaa gccgtacatt
     2641 gggaaccgga acccgtacat tgggaaccca aagccgtaca ttgggaaccg gtcacacatg
     2701 taagtgactg atataaaaga gaaaaaaggc gatttttccg cctaaaactc tttaaaactt
     2761 attaaaactc ttaaaacccg cctggcctgt gcataactgt ctggccagcg cacagccgaa
     2821 gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt
     2881 cggcctctcg agtgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     2941 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     3001 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3061 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3121 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3181 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3241 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3301 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     3361 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3421 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3481 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     3541 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     3601 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     3661 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     3721 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     3781 cgaaaactca cgttaaggga ttttggtcat gcatctaggt actaaaacaa ttcatccagt
     3841 aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc
     3901 tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca
     3961 taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct
     4021 ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc
     4081 ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc
     4141 cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag
     4201 cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg
     4261 atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc
     4321 gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca
     4381 aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc
     4441 cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt
     4501 tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg
     4561 cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat
     4621 tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga
     4681 cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt
     4741 caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccac
     4801 aattatgcat cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc
     4861 gagatcatcc gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta
     4921 acatgagcaa agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg
     4981 ggctgcctgt atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg
     5041 ctggtggcag gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat
     5101 tgcggacgtt tttaatgtac tgaattaacg ccgaattatt ccagaatgag ttgtcaaacc
     5161 agtaggattt ctctgcatgt cgtgtcactt gcattttaag gacagattaa cggttatgac
     5221 tagggtcggt aaagcaaata aatcgacaaa attagaaaac aaagaaaatg tggaaaaagc
     5281 agtcaaaaga agaaaaatgt tgacaaatgt tacgaatttg gtttaaatca aaagtgaatt
     5341 atccgagaaa gatgatattt acccccaaaa tatctaaaca aatacttaaa aatgcaattc
     5401 tctaatagac tatcaaatat cccgatacct ctttatatag tgccatcttc atccttagta
     5461 atgtacacac acacacataa cacttatttc caactctgtc tctctcaatt ttctttctct
     5521 ttctccaacc atgacctcct ccgagaacgt catnncgaag ttcatgcgct tcaaggtgcg
     5581 catggagggc accgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     5641 ctacgagggc cacaacaccg tgaagctgaa ggtgaccaag ggcggccccc tgcccttcgc
     5701 ctgggacatc ctgtcccccc agttccagta cggctccaag gtgtacgtga agcaccccgc
     5761 cgacatcccc gactacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     5821 gaacttcgag gacggcggcg tggcgaccgt gacccaggac tcctccctgc aggacggctg
     5881 cttcatctac aaggtgaagt tcatcggcgt gaacttcccc tccgacggcc ccgtgatgca
     5941 gaagaagaca atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct
     6001 gaagggcgag acccacaagg ccctgaagct gaaggacggc ggccactacc tggtggagtt
     6061 caagtccatc tacatggcca agaagcccgt gcagctgccc ggctactact acgtggacgc
     6121 caagctggac atcacctccc acaacgagga ctacaccatc gtggagcagt acgagcgcac
     6181 cgagggccgc caccacctgt tcctctagga tcgttcaaac atttggcaat aaagtttctt
     6241 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     6301 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     6361 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     6421 ggataaatta tcgcgcgcgg tgtcatctat gttactagat ctctgaattc acgtggtacc
     6481 gcggccgcag atctaagctt gcatgcctgc aggtcgatcc cccctcagaa gaccagaggg
     6541 ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag
     6601 ctatctgtca cttcatcgaa aggacagtag aaaaggaagg tggctcctac aaatgccatc
     6661 attgcgataa aggaaaggct atcgttcaag atgcctctac cgacagtggt cccaaagatg
     6721 gacccccacc cacgaggaac atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
     6781 aagtggattg atgtgatggc cgctctagca tggagtcaaa gattcaaata gaggacctaa
     6841 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
     6901 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
     6961 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
     7021 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
     7081 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
     7141 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     7201 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     7261 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     7321 ttcatttgga gaggacaggc ttcttgagat ccttcaacaa ttaccaacaa caacaaacaa
     7381 caaacaacat tacaattact atttacaatt acaccatggt ccgtcctgta gaaaccccaa
     7441 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg
     7501 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag
     7561 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt
     7621 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
     7681 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg
     7741 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
     7801 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
     7861 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
     7921 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
     7981 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
     8041 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
     8101 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
     8161 tctatgaact gtgcgtcaca gccaaaagcc agacagagtg tgatatctac ccgcttcgcg
     8221 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct
     8281 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg
     8341 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
     8401 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
     8461 ttgatgaaac tgctgctgtc ggctttaacc tctctttagg cattggtttc gaagcgggca
     8521 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
     8581 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
     8641 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
     8701 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
     8761 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
     8821 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac
     8881 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata
     8941 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
     9001 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
     9061 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
     9121 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga
     9181 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaatgaatc aacaactctc
     9241 ctggcgcacc atcgtcggct acagcctcgg tgggggatcc tcctctagag tccgcaaaaa
     9301 tcaccagtct ctctctacaa atctatctct ctctattttt ctccagaata atgtgtgagt
     9361 agttcccaga taagggaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa
     9421 gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc
     9481 taaaaccaaa atccagtgac ctgcaggcat gcaagcttag cttgagcttg gatcagattg
     9541 tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
     9601 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
     9661 ccgttcgtcc atttgtatgt gcatgccaac cacagggttc ccctcgggat caa
//
