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pMA632 vector (V004851)

Basic Vector Information

Vector Name:
pMA632
Antibiotic Resistance:
Ampicillin
Length:
4075 bp
Type:
Dual pho-lac fusion vector
Replication origin:
ori
Source/Author:
Alexeyev MF, Winkler HH.

pMA632 vector Vector Map

pMA6324075 bp60012001800240030003600f1 oripolylinkerM13 fwdpolylinkeroriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMA632 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_29626        4075 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Dual pho-lac fusion vector pMA632, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Alexeyev MF, Winkler HH.
  TITLE     Membrane topology of the Rickettsia prowazekii ATP/ADP translocase 
            revealed by novel dual pho-lac reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Alexeyev MF.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-1998) Microbiology and Immunology, University of 
            South Alabama, LMB Building, Mobile, AL 36688-0001, USA
REFERENCE   3  (bases 1 to 4075)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4075)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-AUG-1998) Microbiology and Immunology, University of South 
            Alabama, LMB Building, Mobile, AL 36688-0001, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4075
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     misc_feature    532..551
                     /label=polylinker
                     /note="polylinker"
     primer_bind     complement(1909..1925)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2070..2089
                     /label=polylinker
                     /note="polylinker"
     rep_origin      complement(2328..2916)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3090..3947)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3948..4052)
                     /label=AmpR promoter

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