LOCUS       40924_29626        4075 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Dual pho-lac fusion vector pMA632, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Alexeyev MF, Winkler HH.
  TITLE     Membrane topology of the Rickettsia prowazekii ATP/ADP translocase 
            revealed by novel dual pho-lac reporters
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Alexeyev MF.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-1998) Microbiology and Immunology, University of 
            South Alabama, LMB Building, Mobile, AL 36688-0001, USA
REFERENCE   3  (bases 1 to 4075)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4075)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-AUG-1998) Microbiology and Immunology, University of South 
            Alabama, LMB Building, Mobile, AL 36688-0001, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4075
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     misc_feature    532..551
                     /label=polylinker
                     /note="polylinker"
     primer_bind     complement(1909..1925)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2070..2089
                     /label=polylinker
                     /note="polylinker"
     rep_origin      complement(2328..2916)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3090..3947)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3948..4052)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc acccgggcga
      541 gctccaggcc ttgggccctg ttctggaaaa ccgggctgct cagggcgata ttactgcacc
      601 cggcggtgct cgccgtttaa cgggtgatca gactgccgct ctgcgtgatt ctcttagcga
      661 taaacctgca aaaaatatta ttttgctgat tggcgatggg atgggggact cggaaattac
      721 tgccgcacgt aattatgccg aaggtgcggg cggctttttt aaaggtatag atgccttacc
      781 gcttaccggg caatacactc actatgcgct gaataaaaaa accggcaaac cggactacgt
      841 caccgactcg gctgcatcag caaccgcctg gtcaaccggt gtcaaaacct ataacggcgc
      901 gctgggcgtc gatattcacg aaaaagatca cccaacgatt ctggaaatgg caaaagccgc
      961 aggtctggcg accggtaacg tttctaccgc agagttgcag gatgccacgc ccgctgcgct
     1021 ggtggcacat gtgacctcgc gcaaatgcta cggtccgagc gcgaccagtg aaaaatgtcc
     1081 gggtaacgct ctggaaaaag gcggaaaagg atcgattacc gaacagctgc ttaacgctcg
     1141 tgccgacgtt acgcttggcg gcggcgcaaa aacctttgct gaaacggcaa ccgctggtga
     1201 atggcaggga aaaacgctgc gtgaacaggc acaggcgcgt ggttatcagt tggtgagcga
     1261 tgctgcctca ctgaattcgg tgacggaagc gaatcagcaa aaacccctgc ttggcctgtt
     1321 tgctgacggc aatatgccag tgcgctggct aggaccgaaa gcaacgtacc atggcaatat
     1381 cgataagccc gcagtcacct gtacgccaaa tccgcaacgt aatgacagtg taccaaccct
     1441 ggcgcagatg accgacaaag ccattgaatt gttgagtaaa aatgagaaag gctttttcct
     1501 gcaagttgaa ggtgcgtcaa tcgataaaca ggatcatgct gcgaatcctt gtgggcaaat
     1561 tggcgagacg gtcgatctcg atgaagccgt acaacgggcg ctggaattcg ctaaaaagga
     1621 gggtaacacg ctggtcatag tcaccgctga tcacgcccac gccagccaga ttgttgcgcc
     1681 ggataccaaa gctccgggcc tcacccaggc gctaaatacc aaagatggcg cagtgatggt
     1741 gatgagttac gggaactccg aagaggattc acaagaacat accggcagtc agttgcgtat
     1801 tgcggcgtat ggcccgcatg ccgccaatgt tgttggactg accgaccaga ccgatctctt
     1861 ctacaccatg aaagccgctc tggggctgaa aggtaccatt acggattcac tggccgtcgt
     1921 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     1981 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     2041 gttgcgcagc ctgaatggcg aatggcccat cgcgacgata tcaggcgcct gctgcattaa
     2101 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     2161 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     2221 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     2281 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     2341 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     2401 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     2461 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     2521 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     2581 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     2641 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     2701 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     2761 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     2821 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2881 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2941 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     3001 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     3061 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     3121 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     3181 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     3241 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     3301 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     3361 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     3421 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     3481 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     3541 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     3601 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     3661 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     3721 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     3781 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3841 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3901 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3961 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     4021 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccac
//
