Basic Vector Information
- Vector Name:
- PM-YC3.6-LTI6b
- Antibiotic Resistance:
- Streptomycin
- Length:
- 13001 bp
- Type:
- Binary expression vector
- Replication origin:
- ori
- Source/Author:
- Krebs M, Held K, Binder A, Hashimoto K, Den Herder G, Parniske M, Kudla J, Schumacher K.
- Promoter:
- MAS
PM-YC3.6-LTI6b vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
PM-YC3.6-LTI6b vector Sequence
LOCUS 40924_29586 13001 bp DNA circular SYN 18-DEC-2018 DEFINITION Binary expression vector PM-YC3.6-LTI6b, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 13001) AUTHORS Krebs M, Held K, Binder A, Hashimoto K, Den Herder G, Parniske M, Kudla J, Schumacher K. TITLE FRET-based genetically encoded sensors allow high-resolution live cell imaging of Ca(2) dynamics JOURNAL Plant J. 69 (1), 181-192 (2012) PUBMED 21910770 REFERENCE 2 (bases 1 to 13001) AUTHORS Krebs M, Held K, Binder A, Hashimoto K, Den Herder G, Parniske M, Kudla J, Schumacher K. TITLE Direct Submission JOURNAL Submitted (24-OCT-2011) Department of Developmental Biology, Centre for Organismal Studies Heidelberg, Im Neuenheimer Feld 230, Heidelberg 69120, Germany REFERENCE 3 (bases 1 to 13001) TITLE Direct Submission REFERENCE 4 (bases 1 to 13001) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; date: "2012"; volume: "69"; issue: "1"; pages: "181-192" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (24-OCT-2011) Department of Developmental Biology, Centre for Organismal Studies Heidelberg, Im Neuenheimer Feld 230, Heidelberg 69120, Germany" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..13001 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature complement(114..138) /label=RB T-DNA repeat /note="right border repeat from nopaline C58 T-DNA" protein_bind 303..324 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 339..369 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 377..393 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 401..417 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" regulatory 444..1089 /label=AtUBQ10 /note="AtUBQ10" /regulatory_class="promoter" CDS 1108..1791 /label=CFP /note="cyan variant of GFP" CDS 2248..2325 /codon_start=1 /product="calmodulin-binding peptide" /label=CBP /note="derived from skeletal muscle myosin light chain kinase; binds calmodulin with nanomolar affinity in the presence of calcium" /translation="KRRWKKNFIAVSAANRFKKISSSGAL" CDS 2548..3066 /codon_start=1 /product="N-terminal fragment of mVenus for use in bimolecular fluorescence complementation (BiFC) (Kodama and Hu, 2010)" /label=VN173 /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK ANFKIRHNIE" misc_feature 3124..3288 /label=Lti6b /note="Lti6b" terminator 3304..3945 /label=E9 terminator /note="terminator and polyadenylation signal from the pea rbcS-E9 gene" primer_bind complement(3958..3974) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" terminator complement(4556..4808) /label=MAS terminator /note="mannopine synthase terminator" CDS complement(5007..5798) /label=NeoR/KanR /note="aminoglycoside phosphotransferase" promoter complement(5828..6208) /label=MAS promoter /note="mannopine synthase promoter (Velten et al., 1984)" misc_feature complement(6580..6604) /label=LB T-DNA repeat /note="left border repeat from nopaline C58 T-DNA" CDS 7132..7920 /label=SmR /note="aminoglycoside adenylyltransferase (Murphy, 1985)" rep_origin 8167..8755 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" misc_feature complement(8941..9081) /label=bom /note="basis of mobility region from pBR322" rep_origin complement(9425..9619) /direction=LEFT /label=pVS1 oriV /note="origin of replication for the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" CDS complement(9688..10752) /label=pVS1 RepA /note="replication protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)" CDS complement(11189..11815) /label=pVS1 StaA /note="stability protein from the Pseudomonas plasmid pVS1 (Heeb et al., 2000)"
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