LOCUS       40924_29586       13001 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Binary expression vector PM-YC3.6-LTI6b, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13001)
  AUTHORS   Krebs M, Held K, Binder A, Hashimoto K, Den Herder G, Parniske M, 
            Kudla J, Schumacher K.
  TITLE     FRET-based genetically encoded sensors allow high-resolution live 
            cell imaging of Ca(2) dynamics
  JOURNAL   Plant J. 69 (1), 181-192 (2012)
  PUBMED    21910770
REFERENCE   2  (bases 1 to 13001)
  AUTHORS   Krebs M, Held K, Binder A, Hashimoto K, Den Herder G, Parniske M, 
            Kudla J, Schumacher K.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-OCT-2011) Department of Developmental Biology, Centre 
            for Organismal Studies Heidelberg, Im Neuenheimer Feld 230, 
            Heidelberg 69120, Germany
REFERENCE   3  (bases 1 to 13001)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 13001)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2012"; volume: "69"; issue: "1"; pages: "181-192"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-OCT-2011) Department of Developmental Biology, Centre for 
            Organismal Studies Heidelberg, Im Neuenheimer Feld 230, Heidelberg 
            69120, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..13001
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(114..138)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    303..324
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        339..369
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    377..393
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     401..417
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      444..1089
                     /label=AtUBQ10
                     /note="AtUBQ10"
                     /regulatory_class="promoter"
     CDS             1108..1791
                     /label=CFP
                     /note="cyan variant of GFP"
     CDS             2248..2325
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             2548..3066
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     misc_feature    3124..3288
                     /label=Lti6b
                     /note="Lti6b"
     terminator      3304..3945
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(3958..3974)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(4556..4808)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(5007..5798)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(5828..6208)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    complement(6580..6604)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7132..7920
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     rep_origin      8167..8755
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8941..9081)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(9425..9619)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9688..10752)
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11189..11815)
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgttattc taataaacgc tcttttctct taggtttacc
      121 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      181 tccaagctca agctgctcta gccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      241 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      301 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      361 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
      421 gattacgaat tcgagctcgg tacccgacga gtcagtaata aacggcgtca aagtggttgc
      481 agccggcaca cacgagtcgt gtttatcaac tcaaagcaca aatacttttc ctcaacctaa
      541 aaataaggca attagccaaa aacaactttg cgtgtaaaca acgctcaata cacgtgtcat
      601 tttattatta gctattgctt caccgcctta gctttctcgt gacctagtcg tcctcgtctt
      661 ttcttcttct tcttctataa aacaataccc aaagagctct tcttcttcac aattcagatt
      721 tcaatttctc aaaatcttaa aaactttctc tcaattctct ctaccgtgat caaggtaaat
      781 ttctgtgttc cttattctct caaaatcttc gattttgttt tcgttcgatc ccaatttcgt
      841 atatgttctt tggtttagat tctgttaatc ttagatcgaa gacgattttc tgggtttgat
      901 cgttagatat catcttaatt ctcgattagg gtttcataga tatcatccga tttgttcaaa
      961 taatttgagt tttgtcgaat aattactctt cgatttgtga tttctatcta gatctggtgt
     1021 tagtttctag tttgtgcgat cgaatttgta gattaatctg agtttttctg attaacagct
     1081 cgagtgcggg atcctgcggc cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
     1141 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaggtt cagcgtgtcc
     1201 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     1261 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc
     1321 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     1381 ggctacgtcc aggagcgtac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     1441 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     1501 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacatcag ccacaacgtc
     1561 tatatcaccg ccgacaagca gaagaacggc atcaaggccc acttcaagat ccgccacaac
     1621 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     1681 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     1741 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc ccgcatgcat
     1801 gaccaactga cagaagagca gattgcagag ttcaaagaag ccttctcatt attcgacaag
     1861 gatggggacg gcaccatcac cacaaaggaa cttggcaccg ttatgaggtc gcttggacaa
     1921 aacccaacgg aagcagaatt gcaggatatg atcaatgaag tcgatgctga tggcaatgga
     1981 acgatttact ttcctgaatt tcttactatg atggctagaa aaatgaagga cacagacagc
     2041 gaagaggaaa tccgagaagc attccgtgtt tttgacaagg atgggaacgg ctacatcagc
     2101 gctgctcagt tacgtcacgt catgacaaac ctcggggaga agttaacaga tgaagaagtt
     2161 gatgaaatga taagggaagc agatatcgat ggtgatggcc aagtaaacta tgaagagttt
     2221 gtacaaatga tgacagcaaa gggggggaag aggcgctgga agaaaaactt cattgccgtc
     2281 agcgctgcca accggttcaa gaagatctcc agctccgggg cactggagct catggacggc
     2341 ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     2401 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag
     2461 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     2521 gagctgtaca agggtggcag cggtggcatg gtgagcaagg gcgaggagct gttcaccggg
     2581 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     2641 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc
     2701 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc
     2761 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     2821 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     2881 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     2941 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     3001 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac
     3061 atcgagttgt ctagagggcc cgggacgtcc gcggagatct acgcgtgtcg acaagcagca
     3121 gcaatgagta cagccacttt cgtagagatt attcttgcta tcatcttgcc tcctctcggc
     3181 gtctttctca aatttggttg caaggttgag ttttggatat gtttgatttt gacgctgttt
     3241 ggttatcttc ccggaatcct ttacgctctt tatatcatca ccaagtgaac tagttggctg
     3301 cagagctttc gttcgtatca tcggtttcga caacgttcgt caagttcaat gcatcagttt
     3361 cattgcgcac acaccagaat cctactgagt tcgagtatta tggcattggg aaacatgttt
     3421 ttcttgtacc atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg
     3481 aactgtgaaa tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt
     3541 ctcaaattaa tattatttgt tttttctctt atttgttgtg tgttgaattt gaaaatataa
     3601 gagatatgca aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg
     3661 aagttaatat gaggagtaaa acacttgtag ttgtaccatt atgcttattc actaggcaac
     3721 aaatatattt tcagacctag aaaagctgca aatgttactg aatacaagta tgtcctcttg
     3781 tgttttagac atttatgaac tttcctttat gtaattttcc agaatccttg tcagattcta
     3841 atcattgctt tataattata gttatactca tggatttgta gttgagtatg aaaatatttt
     3901 ttaatgcatt ttatgacttg ccaattgatt gacaacatgc atcaatcgaa gcttggcact
     3961 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     4021 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     4081 ttcccaacag ttgcgcagcc tgaatggcga atgctagagc agcttgccaa tcgaggccga
     4141 ccaaccgcaa gcgttgtcag tgttgcaaag cgctctgtgt gggcctactt taattgcttc
     4201 cagtgttaaa ttggcgaaag gcaataatat cgcaaaatat tgtgttgtaa aatgtaatta
     4261 tgttttaatt tcatggaaat gtttgagcat aatttttatt aatgtactaa attactgttt
     4321 tgttaaatgc aattttgctt tctcgggatt ttaatatcaa aatctattta gaaatacaca
     4381 atattttgtt gcaggcttgc tggagaatcg atctgctatc ataaaaatta caaaaaaatt
     4441 ttatttgcct caattatttt aggattggta ttaaggacgc ttaaattatt tgtcgggtca
     4501 ctacgcatca ttgtgattga gaagatcagc gatacgaaat attcgtagta ctatcgataa
     4561 tttatttgaa aattcataag aaaagcaaac gttacatgaa ttgatgaaac aatacaaaga
     4621 cagataaagc cacgcacatt taggatattg gccgagatta ctgaatattg agtaagatca
     4681 cggaatttct gacaggagca tgtcttcaat tcagcccaaa tggcagttga aatactcaaa
     4741 ccgccccata tgcaggagcg gatcattcat tgtttgtttg gttgcctttg ccaacatggg
     4801 agtccaaggt ttcagggaag ctggaattcc ggggtgggcg aagaactcca gcatgagatc
     4861 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc
     4921 atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt
     4981 catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg
     5041 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg
     5101 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca
     5161 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc
     5221 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc
     5281 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg
     5341 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg
     5401 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat
     5461 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat
     5521 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc
     5581 gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgga gttcattcag ggcaccggac
     5641 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca
     5701 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg
     5761 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc ggggaattcg
     5821 ctagagtcga tttggtgtat cgagattggt tatgaaattc agatgctagt gtaatgtatt
     5881 ggtaatttgg gaagatataa taggaagcaa ggctatttat ccatttctga aaaggcgaaa
     5941 tggcgtcacc gcgagcgtca cgcgcattcc gttcttgctg taaagcgttg tttggtacac
     6001 ttttgactag cgaggcttgg cgtgtcagcg tatctattca aaagtcgtta atggctgcgg
     6061 atcaagaaaa agttggaata gaaacagaat acccgcgaaa ttcaggcccg gttgccatgt
     6121 cctacacgcc gaaataaacg accaaattag tagaaaaata aaaactgact cggatactta
     6181 cgtcacgtct tgcgcactga tttgaaaaat ctcaatataa acaaagacgg ccacaagaaa
     6241 aaaccaaaac accgatattc attaatctta tctagtttct caaaaaaatt catatcttcc
     6301 acacgtgaaa atgccaattt ctcagaccta cctcggctct gcgaaggccc ccgctggtat
     6361 caaaagtttt tatttcatcc gacatggcgc gaccgacctc aacgagaagg aaattgtcgt
     6421 gaacggtgag aagctctggg gcgtgcaagg ttccggaacg aacatcggtc tcaatgcaaa
     6481 aggggaacgc caggctctgt tggcccctcg aaattcggcg ttaattcagt acattaaaaa
     6541 cgtccgcaat gtgttattaa gttgtctaag cgtcaatttg tttacaccac aatatatcct
     6601 gccaccagcc agccaacagc tccccgaccg gcagctcggc acaaaatcac cactcgatac
     6661 aggcagccca tcagtccggg acggcgtcag cgggagagcc gttgtaaggc ggcagacttt
     6721 gctcatgtta ccgatgctat tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg
     6781 atgatctcgc ggagggtagc atgttgattg taacgatgac agagcgttgc tgcctgtgat
     6841 caattcgggc acgaacccag tggacataag cctcgttcgg ttcgtaagct gtaatgcaag
     6901 tagcgtaact gccgtcacgc aactggtcca gaaccttgac cgaacgcagc ggtggtaacg
     6961 gcgcagtggc ggttttcatg gcttcttgtt atgacatgtt tttttggggt acagtctatg
     7021 cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc
     7081 agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat catgggggaa
     7141 gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat
     7201 ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag
     7261 ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg
     7321 cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc
     7381 cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct
     7441 aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag
     7501 ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt
     7561 gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt
     7621 gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag
     7681 cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg
     7741 ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc
     7801 atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca
     7861 gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa
     7921 taatgtctag ctagaaattc gttcaagccg acgccgcttc gccggcgtta actcaagcga
     7981 ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt ctgcttttat
     8041 tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat gcatgaccaa
     8101 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8161 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8221 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8281 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     8341 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8401 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8461 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8521 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8581 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8641 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8701 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8761 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8821 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     8881 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     8941 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
     9001 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     9061 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     9121 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     9181 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg
     9241 ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag
     9301 gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag
     9361 ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc gctggccaga
     9421 cagttatgca caggccaggc gggttttaag agttttaata agttttaaag agttttaggc
     9481 ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat
     9541 gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc
     9601 aatgtacgtg ctatccacag gaaagagacc ttttcgacct ttttcccctg ctagggcaat
     9661 ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt
     9721 gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc
     9781 cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc
     9841 ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc
     9901 ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc
     9961 ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc
    10021 agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg
    10081 gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt
    10141 acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt
    10201 ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa
    10261 cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg
    10321 ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc
    10381 ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc
    10441 acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac
    10501 gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat
    10561 cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc
    10621 ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc
    10681 ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga
    10741 tggtttgcga ccgtcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc
    10801 ggcagacaac ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt
    10861 ccacggcgtc ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa
    10921 ttcatctact catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat
    10981 agcagctcgg taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc
    11041 tccgccggca actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac
    11101 gttgcagcct tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg
    11161 ctcattttct ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg
    11221 cctggacctc gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg
    11281 cggcagtgcc tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt
    11341 acccggccag cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca
    11401 cgacaaaggc cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca
    11461 ccaggtcggc ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt
    11521 cggctgcctt gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta
    11581 cgaagtcgcg ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga
    11641 caacggttag cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat
    11701 cggaatcgac taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg
    11761 cgatggtcgt cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt
    11821 ccccttgcgt atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa
    11881 gctgttttac tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc
    11941 caagctggcc ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag
    12001 ccgcacggtt gagacgtgcg cgggcggctc gaacacgtac ccggccgcga tcatctccgc
    12061 ctcgatctct tcggtaatga aaaacggttc gtcctggccg tcctggtgcg gtttcatgct
    12121 tgttcctctt ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc
    12181 tcacggaagg caccgcgccg cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt
    12241 gcgcggtaca gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg
    12301 ccttcctggt cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg
    12361 gggccaaact tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg
    12421 attagggaac gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc
    12481 ggcgtggtgg tgtcggccca cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg
    12541 atgcgctcgg caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc
    12601 actgtcacaa cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg
    12661 cgcctggtgc cggtgatctt ctcggaaaac agcttggtgc agccggccgc gtgcagttcg
    12721 gcccgttggt tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca
    12781 gcggcggcgc tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt attctacttt
    12841 atgcgactaa aacacgcgac aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc
    12901 gtaacttagg acttgtgcga catgtcgttt tcagaagacg gctgcactga acgtcagaag
    12961 ccgactgcac tatagcagcg gaggggttgg atcaaagtac t
//
