pLX-Z4 vector (V004873)

MSDS

Basic Vector Information

Vector Name:: pLX-Z4

Antibiotic Resistance:: Gentamycin

Length:: 3740 bp

Type:: Cloning vector

Replication origin:: oriV

Source/Author:: Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez D, Garcia JA.

Promoter:: lac UV5

pLX-Z4 vector Vector Map

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Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pLX-Z4 vector Sequence

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Download GeneBank File(.gb)

LOCUS       40924_29451        3740 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLX-Z4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez 
            D, Garcia JA.
  TITLE     Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors 
            with Compatible Replication Origins
  JOURNAL   ACS Synth Biol (2017) In press
  PUBMED    28657330
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Pasin F, Garcia JA.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2017) Department of Plant Molecular Genetics, 
            Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3740)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3740)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-MAR-2017) Department of Plant Molecular Genetics, Centro 
            Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid 
            28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      27..58
                     /regulatory_class="terminator"
     misc_feature    59..83
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    complement(106..129)
                     /label=LB
                     /note="LB"
     CDS             complement(226..396)
                     /label=lacZ-alpha
                     /note="LacZ-alpha fragment of beta-galactosidase"
     promoter        complement(445..475)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     misc_feature    complement(563..586)
                     /note="right border of right T-DNA; RB"
     regulatory      complement(657..709)
                     /regulatory_class="terminator"
     CDS             complement(773..1303)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(1492..1520)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_feature    1521..1544
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(1598..2743)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     rep_origin      complement(3136..3667)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"

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