LOCUS       40924_29451        3740 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pLX-Z4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3740)
  AUTHORS   Pasin F, Bedoya LC, Bernabe-Orts JM, Gallo A, Simon-Mateo C, Orzaez 
            D, Garcia JA.
  TITLE     Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors 
            with Compatible Replication Origins
  JOURNAL   ACS Synth Biol (2017) In press
  PUBMED    28657330
REFERENCE   2  (bases 1 to 3740)
  AUTHORS   Pasin F, Garcia JA.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-MAR-2017) Department of Plant Molecular Genetics, 
            Centro Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, 
            Madrid 28049, Spain
REFERENCE   3  (bases 1 to 3740)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3740)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2017) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-MAR-2017) Department of Plant Molecular Genetics, Centro 
            Nacional de Biotecnologia (CNB-CSIC), Darwin 3, Madrid, Madrid 
            28049, Spain"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3740
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      27..58
                     /regulatory_class="terminator"
     misc_feature    59..83
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     misc_feature    complement(106..129)
                     /label=LB
                     /note="LB"
     CDS             complement(226..396)
                     /label=lacZ-alpha
                     /note="LacZ-alpha fragment of beta-galactosidase"
     promoter        complement(445..475)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     misc_feature    complement(563..586)
                     /note="right border of right T-DNA; RB"
     regulatory      complement(657..709)
                     /regulatory_class="terminator"
     CDS             complement(773..1303)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(1492..1520)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     misc_feature    1521..1544
                     /label=assembly linker
                     /note="assembly linker"
     CDS             complement(1598..2743)
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     rep_origin      complement(3136..3667)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
ORIGIN
        1 caagaagatc ctttgatctt ttctacaggc ctgctggtaa tcgcaggcct ttttattttg
       61 gcaggatata ttgtggtgta aacacttacc gcacctctgc agcagcggca ggatatatgg
      121 cagtgtaaac tccattttcg aacgcgttaa ttaagtagcc tggtcgaaac cgtctcagga
      181 gagagaccaa aagcaaaaac ccgccgaagc gggttactag cgccattcgc cattcagaga
      241 gcggagctgc tgcgacggac gatcggtacg cgcctcttcg ctattacgcc aactggcgaa
      301 aggtggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
      361 ttgtagtacc acggcaaggc tatctgtaat cattgttgta ctccggttag gacggattgg
      421 gaactggcta actcaaaatc cacacattat acgagccgga agcataaagt gtaaagcctg
      481 gggtgcctaa tgagtgaggt ctctcgctgt caggagacgg gacaaggatg cgcctgcagg
      541 ttgttgatga tgtgatgact gatggcagga tatatgtggt tgtaattcat ttctaccgtg
      601 taatttactg tatttttttg tttgttcgtt cgtttgtaaa aatatttttt ggaagcaaaa
      661 aattagcgca agaagacaaa aatcaccttg cgctaatgct ctgttacagg cgcgccaatt
      721 tacccaacaa ctccgcggcc gggaagccga tctcggcttg aacgaattgt taggtggcgg
      781 tacttgggtc gatatcaaag tgcatcactt cttcccgtat gcccaacttt gtatagagag
      841 ccactgcggg atcgtcaccg taatctgctt gcacgtagat cacataagca ccaagcgcgt
      901 tggcctcatg cttgaggaga ttgatgagcg cggtggcaat gccctgcctc cggtgctctc
      961 cggagactgc gagatcatag atatagatct cactacgcgg ctgctcaaac ttgggcagaa
     1021 cgtaagccgc gagagcgcca acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg
     1081 tcttactacg gagcaagttc ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg
     1141 ctacatcgcc gaactcacga ccgaaaagat caagagcagc ccgcatggat ttgacttggt
     1201 cagggccgag cctacatgtg cgaatgatgc ccatacttga gccacctaac tttgttttag
     1261 ggcgactgcc ctgctgcgta acatcgttgc tgctgcgtaa catcgttgct gctccataac
     1321 atcaaacatc gacccacggc gtaacgcgct tgctgcttgg atgcccgagg cataggctgt
     1381 acaaaaaaac agtcataaca agccatgaaa accgccactg cgccgttacc accgctgcgt
     1441 tcggtcaagg ttctggacca gttgcgtgag cgcatacgct acttgcatta cagtttacga
     1501 accgaacagg cttatgtcaa gatcggattg gcggttatgc ggttgcgatg caggtggctg
     1561 ctgaaccccc agccggaact gaccccacaa ggccctagcg tttgcaatgc accaggtcat
     1621 cattgaccca ggcgtgttcc accaggccgc tgcctcgcaa ctcttcgcag gcttcgccga
     1681 cctgctcgcg ccacttcttc acgcgggtgg aatccgatcc gcacatgagg cggaaggttt
     1741 ccagcttgag cgggtacggc tcccggtgcg agctgaaata gtcgaacatc cgtcgggccg
     1801 tcggcgacag cttgcggtac ttctcccata tgaatttcgt gtagtggtcg ccagcaaaca
     1861 gcacgacgat ttcctcgtcg atcaggacct ggcaacggga cgttttcttg ccacggtcca
     1921 ggacgcggaa gcggtgcagc agcgacaccg attccaggtg cccaacgcgg tcggacgtga
     1981 agcccatcgc cgtcgcctgt aggcgcgaca ggcattcctc ggccttcgtg taataccggc
     2041 cattgatcga ccagcccagg tcctggcaaa gctcgtagaa cgtgaaggtg atcggctcgc
     2101 cgataggggt gcgcttcgcg tactccaaca cctgctgcca caccagttcg tcatcgtcgg
     2161 cccgcagctc gacgccggtg taggtgatct tcacgtcctt gttgacgtgg aaaatgacct
     2221 tgttttgcag cgcctcgcgc gggattttct tgttgcgcgt ggtgaacagg gcagagcggg
     2281 ccgtgtcgtt tggcatcgct cgcatcgtgt ccggccacgg cgcaatatcg aacaaggaaa
     2341 gctgcatttc cttgatctgc tgcttcgtgt gtttcagcaa cgcggcctgc ttggcttcgc
     2401 tgacctgttt tgccaggtcc tcgccggcgg tttttcgctt cttggtcgtc atagttcctc
     2461 gcgtgtcgat ggtcatcgac ttcgccaaac ctgccgcctc ctgttctagc cgacgcgaac
     2521 gctccacggc ggccgatggc gcgggcaggg cagggggagc cagttgcacg ctgtcgcgct
     2581 cgatcttggc cgtagcttgc tggactatcg agccgacgga ctggaaggtt tcgcggggcg
     2641 cacgcatgac ggtgcggctt gcgatggttt cggcatcctc ggcggaaaac cccgcgtcga
     2701 tcagttcttg cctgtatgcc ttccggtcaa acgtccgatt cattcaccct ccttgcggga
     2761 ttgccccgga attaattccc cggatcgatc cgtcgatctt gatcccctgc gccatcagat
     2821 ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg
     2881 cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt ctagctatcg
     2941 ccatgtaagc ccactgcaag ctacctgctt tctctttgcg cttgcgtttt cccttgtcca
     3001 gatagcccag tagctgacat tcatccgggg tcagcaccgt ttctgcggac tggctttcta
     3061 cgtggctgcc atttttgggg tgaggccgtt cgcggccgag gggcgcagcc cctgggggga
     3121 tgggaggccc gcgttagcgg gccgggaggg ttcgagaagg gggggcaccc cccttcggcg
     3181 tgcgcggtca cgcgcacagg gcgcagccct ggttaaaaac aaggtttata aatattggtt
     3241 taaaagcagg ttaaaagaca ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa
     3301 tgctggattt tctgcctgtg gacagcccct caaatgtcaa taggtgcgcc cctcatctgt
     3361 cagcactctg cccctcaagt gtcaaggatc gcgcccctca tctgtcagta gtcgcgcccc
     3421 tcaagtgtca ataccgcagg gcacttatcc ccaggcttgt ccacatcatc tgtgggaaac
     3481 tcgcgtaaaa tcaggcgttt tcgccgattt gcgaggctgg ccagctccac gtcgccggcc
     3541 gaaatcgagc ctgcccctca tctgtcaacg ccgcgccggg tgagtcggcc cctcaagtgt
     3601 caacgtccgc ccctcatctg tcagtgaggg ccaagttttc cgcgaggtat ccacaacgcc
     3661 ggcggcccta catggctctg ctgtagtgag tgggttgcgc tccggcagcg gtcctgatcc
     3721 cccgcagaaa aaaaggatct
//
